[PDF] Top 20 Visualization by Live-Cell Microscopy of Disruption of ND10 during Herpes Simplex Virus Type 1 Infection

... for virus infection, human fibroblast HFFF-2 cells were infected with ...after infection with HSV-1 virus vECFP-ICP4, which expresses viral IE protein ICP4 linked to ECFP (14, ...2. ... See full document

... the ND10-associated genomes (39), raise several questions concerning the basis of protein and genome localization juxtaposed to ND10 and whether this has a positive or negative effect on the efficiency of ... See full document

... in live cells at the first time point therefore reflects only the very rapidly diffusing histone molecules, which are considered unbound to chromatin ...than 1 standard deviation above the average level of ... See full document

... in 1% BSA containing 10 ␮g of pooled human Ig (mainly IgG) (Sigma) per ...in 1% BSA as follows: RGST22, 1:500; RGST49, 1:500; affinity-purified RGST49, 1:10; anti-ICP4, 1101 and 1114, ... See full document

... in virus entry, and its mecha- nism of assembly and incorporation into the maturing ...the cell remains to be determined. While it is well established that during virus replication genomic DNA ... See full document

... B23 during the course of HSV-1 ...scanning microscopy analysis were carried out with antibodies to either nucleolin (NUC; red) or ICP8 (green) as a VRC-specific protein and with DAPI (blue) to stain ... See full document

... create cell line HFS-shPML1 or HFS-shLuci, re- ...two cell lines were infected in parallel with in1374 and then stained for ␤ -galactosidase expression the following ...by infection with the ... See full document

... of infection (MOI) of 10 and collecting infected cells at 8 h ...per cell 6 h prior to ...into 1 ml of TBS supplemented with leupeptin, pepstatin, and ...at 1:10,000, 5E10 was used at ... See full document

... by infection, we observed ICP0 expression in basal keratinocytes at 3 h ...p.i. Visualization of ICP0 in tissue sections and epider- mal whole mounts indicated no difference in the efficiency of ... See full document

... affects ND10 and centromeres more slowly in NT2 cells than in HFL ...the cell by inducing the degradation of PML, Sp100, and CENP-C, thereby disrupting both ND10 and centromeres ...of 1 and ... See full document

... fluorescence microscopy, we provide evi- dence that enveloped HSV-1 virions are transported in axons by the neuronal secretory ...reporter virus, approximately 65 to 70% of an- terograde-transported ... See full document

... Following infection of a new cell, release of the DNA would require the penton channels to open ...electron microscopy and low-angle X-ray scattering both indicated that in bacterio- phage, the DNA ... See full document

... at ND10 would be the origin and ICP8, with ICP4 and ICP27 necessary only to transactivate ICP8 from the helper ...at ND10, we transfected cells to express ICP8, fol- lowed by infection with the ... See full document

... HSV-1 infection at various levels has been reviewed recently (53, 56), so for simplicity, the following discussion relates only to the specific instance of ICP0 activity during the early stages of ... See full document

... recombinant herpes simplex virus type 1 (HSV-1) that simultaneously encodes selected structural proteins from all three virion compartments—capsid, tegument, and envelope—fused ... See full document

... in ND10 structures by ...bleached ND10 within 20 s, indicating a rapid exchange of hDaxx molecules between ND10 structures and the surrounding ...bleached ND10 over a period of 3 min ...the ... See full document

... the cell, and the difficulty of isolating it in soluble form have ham- pered attempts to study the nature and biochemical properties of endogenous ...a cell line engineered to express PML at high levels), ... See full document

... from herpes simplex virus type 1 (HSV-1) latently infected mice were used to study ...TG cell cultures. Infectious virus appeared in 75% of culture supernatants ... See full document

... of herpes simplex virus type 1 (HSV-1) incorporated initial circularization of linear molecules in the cell ...lytic infection and moreover that this event is ... See full document

... endosome [34-36]. Taylor et al. [20] introduced envel- ope-deficient retroviral vectors into human rhabdomyo- sarcoma cells, suggesting that an unenveloped-retrovirus could enter the cells through ultrasound-generated ... See full document