[PDF] Top 20 Vitrification of human oocyte using cryoloop
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Vitrification of human oocyte using cryoloop
... After washing all oocytes, they were cultured in same medium for 2 hours. Oocytes with fragmented cytoplasm, indistinct oolemma, increased perivtelline space or damaged zona pellucida were considered as non survivors. ... See full document
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Efficient vitrification of mouse embryos using the Kitasato Vitrification System as a novel vitrification device
... vitrified using the ...the Cryoloop [3], nylon loop [7], open pulled straws [2], the Cryotop [5], and gel-loading tips [6], have been ...for vitrification is to minimize the volume of the ... See full document
9
An Update of Oocyte Vitrification: A Modification of Sucrose and Trehalose as Extracellular Cryoprotectant
... ultra-rapid vitrification for oocyte uses sucrose and trehalose as extracellular cryoprotectant with the technique used is similar to standard slow- freeze vitrification ...toward human ... See full document
6
Factors affecting the outcome of human blastocyst vitrification
... During vitrification, the blastocyst is placed in a loading device surrounded by vitrification ...The Cryoloop is a nylon loop, whereas the Cryotop is a plastic ...of human blastocysts ... See full document
11
Human sperm vitrification: the state of the art
... like cryoloop, a number of closed carriers have been developed and applied in vitri- fication, including straw-in-straw, high security vitrifica- tion straw, Cryotip, VitriSafe, Cryopette, cryoLogic, Rapid-i, S3 ... See full document
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Simplified EM Grid Vitrification Is a Convenient and Efficient Method for Mouse Mature Oocyte Cryopreservation
... injection using 30-gauge tuberculin syringe needles to inject 5 IU pregnant mare serum gona- dotropin (PMSG, Sigma, ...IU human chorionic gonadotropin (hCG, Sigma, USA) 48 hours later, in M16 media ... See full document
6
Vitrification of human ovarian tissue: a practical and relevant alternative to slow freezing
... affecting vitrification properties, relatively low con- centrations of different cryoprotectants can be combined ...of oocyte and embryo vitrification ...successful vitrification of preantral ... See full document
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Original Article Vitrification of mouse oocyte versus slow freezing: evaluation of ultrastructure and developmental potential
... of oocyte cryopreservation techniques have lead to major breakthroughs in human assisted reproductive technology, but the effect of cryopreserving oocytes is not ...the vitrification and slow ... See full document
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... Hepes-buffered human tubal fluid medium (HTF) (sigma) supplemented with 5mg/ml ...cumulus oocyte complexes” were randomly allocated to three groups:control without treatment, stepwise preservation and ... See full document
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Sucrose ‘Versus’ Trehalose Cryoprotectant Modification in Oocyte Vitrification : A Study of Embryo Development
... as oocyte and sperm ...IU human chorionic gonadotropin (HCG) (Pregnil, Hoddesdon Hertfortshire, United Kingdom) by intraperitoneal injection, 48 hours ... See full document
8
Vitrification of immature bovine cumulus-oocyte complexes: effects of cryoprotectants, the vitrification procedure and warming time on cleavage and embryo development
... in human fertility clinics [1] and for conservation of female animal genetic resources ...[2-4]. Oocyte cryopreservation has been performed in several mammalian species; how- ever, success rates have been ... See full document
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Vitrification Increased Vacuolization of Human Spermatozoa: Application of MSOME Technology
... Another factor surveyed in this study was fertili- ty potential of spermatozoa with ZBA assay. This assay highlights the potential of proper sperm in- teraction with oocyte in vitro setting. ZBA assay showed an ... See full document
6
Cryopreservation of human failed-matured oocytes followed by in vitro maturation: vitrification is superior to the slow freezing method
... of oocyte banks. Oocyte preservation can also pro- vide a solution for cancer patients wishing to preserve fertility before undergoing chemotherapy or radiation therapy, or for young cancer patients who do ... See full document
6
Comparing the viability and in vitro maturation of cumulus germinal vesicle break down (GVBD) oocyte complexes using two vitrification techniques in mice
... achieved using frozen and thawed oocyte (4), Fabbri et al (2001) reported a modified slow freezing and rapid thawing method to freeze human oocytes that gave higher rates of oocyte survival ... See full document
6
Oocyte vitrification: A local validation of the method
... Oocyte vitrification has been successfully used world- wide in an Assisted Reproductive technology (ART) laboratory for the past years for various purposes ranging from the diminution of frozen embryo ... See full document
5
Human oocyte maturation in vitro
... Being a monovular species, the cow has also gained increasing importance as a more suitable model for the human, in whom IVM has been introduced to mitigate the unwanted implications of ART. In fact, the current ... See full document
11
De Monte, Erica (2017): Time course and pathologies of in vitro maturation and fertilization of bovine oocytes. Dissertation, LMU München: Tierärztliche Fakultät
... The confocal image stacks of the 654 oocytes were used to determine the incidence of the anomalies characterized and classified in this study. For simplicity, oocytes which had been penetrated by one or more sperm and ... See full document
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The role of recombinant LH in women with hypo-response to controlled ovarian stimulation: a systematic review and meta-analysis
... The reasons for the hypo-responsiveness to gonado- tropin stimulation are not entirely understood, but it has been suggested that genetic mutations or single nucleotide polymorphisms (SNPs) of gonadotropins and their ... See full document
12
Strategies to support human oocyte development in vitro
... that human primordial follicles can be acti- vated and grow well within mechanically loosened cortical pieces, developing to multi-laminar preantral (secondary) stages within 6 days ...of human ovarian ... See full document
8
Comparison of Rapid Freezing and Vitrification for Human Sperm Cryopreservation Using Trehalose as a Cryoprotective Agent
... and vitrification methods in recent years ...and vitrification are also superior to slow freezing in preserving sperm motility and DNA integrity ...and vitrification rely only on non-permeating cryo- ... See full document
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