Adjusting the Selected Parameters

Se recomienda usar otras técnicas analíticas para la cuantificación del etanol como cromatografía de gases y cromatografía liquida de alta calidad (HPLC), ya que la técnica de espectroscopia de infrarrojo cercana es muy sensible ya que está basada en los espectros que se obtienen a partir de las vibraciones de las moléculas y eso se ve reflejado en una desviación estándar alta. Además no permito cuantificar el glicerol presente al final de las fermentaciones ya que los sobretonos característicos del etanol y glicerol son muy similares.

Para posteriores estudios se propone para el gen gapA, sobre-expresar otro gen como adhE que se encuentre al final de la ruta de degradación de glicerol ya que permitiría incrementar aún más la producción de etanol. Y para el gen lpd se recomienda delecionar es decir eliminar el gen pta, pues el gen lpd se encuentra al final de la ruta de degradación de glicerol y convierte el piruvato en acetil CoA, a partir de este producto se derivan mas reacciones una de ellas es la que convierte el acetil CoA en acetilfosfato. Por eso se pretende delecionar genes para que no existan subproductos indeseados.

Como se observo una tendencia en la producción de etanol al aumentar la concentración inicial de glicerol, se recomienda aumentar la concentración inicial de glicerol para posteriores estudios, en busca de mejores resultados.

43

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