When there is a clog in the flowcell or in the Concentric Flow Module (CFM) When the flowcell windows are dirty
Materials required:
• ADVIA OPTIpoint
• ADVIA TESTpoint controls
• EZ KLEEN, PN T01-3624
• Flowcell Cleaning Kit, PN 113-B711-01 Kit includes:
♦ Drill bit
♦ Needle cleaning kit, PN 113-B911-01 - Coupling
- Disposable syringe - Luer adapter
♦ Pin vise
• Household bleach
• Lens tissue
• One 6 inch (15.2 cm) length of 0.020 inch (0.5 mm) ID C-Flex Tubing, PN 562-3052P02
• Spectrophotometric-grade methanol
• Two 50 mL beakers Syringe preparation
Time: Replacement - 10 minutes Checkout - 30 minutes Analyzer mode: Standby
BIOHAZARD
All products or objects that come in contact with human or animal body fluids should be handled, before and after cleaning, as if capable of transmitting infectious diseases. Wear facial protection, gloves, and protective clothing.
The operator should follow the recommendations to prevent the transmission of infectious agents in health-care settings as recommended for potentially
infectious specimens in Protection of Laboratory Workers from Infectious Disease Transmitted by Blood, Body Fluids, and Tissue, 2nd edition; Approved Guideline (1997) Document M29-A, National Committee for Clinical Laboratory Standards (NCCLS). This document contains complete information on user protection and it can be used as reference material for instructions on laboratory safety.
IMPORTANT
An inspection scope must be available for this procedure. Do not remove the flowcells unless you are trained in how to align and focus them.
To remove and clean the flowcells
1. Remove the flowcell. There is a different procedure for each flowcell.
2. Take the flowcell apart.
3. Clean the CFM.
4. Flush the flowcell and the CFM.
5. Clean the glass windows of the flowcell.
6. Replace the flowcell.
7. Check analyzer performance.
See below for detailed steps.
Cleaning the Flowcells off the Analyzer: Step 1 Removing the Perox Flowcell
1. Make sure that the analyzer is in the Standby mode.
2. Open the optics cover. The perox flowcell is on the left.
Perox flowcell (1), RBC flowcell (2)
3. Disconnect the shuttle line (1), the sample-stream input line (2), and the sheath-stream input line (3) from the CFM. Allow these lines to hang freely.
1 SHEATH NIPPLE 2 SAMPLE NIPPLE 3 SHUTTLE NIPPLE
4. Locate hydraulic valve # 24 Unscrew the threaded fitting from the top right side of the valve.
5. Release the flowcell by loosening the release knob located on the right of the flowcell adjustment assembly
Flowcell release knob (1) Front of analyzer
6. Hold the flowcell by the red threaded fitting located at the top of the flowcell, then gently lift the flowcell out of the optics assembly.
CAUTION
To avoid getting fingerprints on the glass windows of the flowcell, always hold the flowcell by the metal slides.
Cleaning the Flowcells off the Analyzer: Step 1 Removing the RBC/BASO/Retic Flowcell
Before you begin the cleaning of the RBC/baso/retic flowcell, read all laser safety precautions.
WARNING
To avoid possible injury that may occur from laser radiation, do not remove the laser safety shield. You must not remove the flowcell if the laser safety shield is not in place on the laser optics assembly.
LASER SAFETY SHIELD DO NOT REMOVE!
LASER WARNING
To avoid damage to the eyes, never look directly at the laser beam or at its reflection from a shiny surface. All field service procedures must be followed precisely. Only Siemens-trained field service personnel should perform procedures related to laser assemblies.
For more safety information and laser specifications, refer to Safety Information, Protecting yourself from lasers.
1. Make sure that the analyzer is in the Standby mode.
2. Open the optics cover. The RBC flowcell is on the left.
Perox flowcell (1), RBC flowcell (2)
3. Disconnect the shuttle line (1), the sample-stream input line (2), and the sheath-stream input line (3) from the CFM. Allow these lines to hang freely.
1 SHEATH NIPPLE 2 SAMPLE NIPPLE 3 SHUTTLE NIPPLE
4. Locate hydraulic valve # 23. Unscrew the threaded fitting from the top left-hand side of the valve.
5. Loosen the captive flowcell release screw, then remove the flowcell.
Flowcell (1), flowcell release screw (2)
6. Hold the flowcell by the red threaded fitting when lifting the flowcell out of the optics assembly.
CAUTION
To avoid getting fingerprints on the glass windows of the flowcell, always hold the flowcell by the metal slides.
Cleaning the Flowcells off the Analyzer: Step 2 Taking the Flowcell Apart
1. Unscrew the locking nut (1) at the input end of the flowcell assembly.
2. Using a twisting motion, remove the CFM (2) from the flowcell.
3. Be careful not to lose the O-ring seal (3).
Cleaning the Flowcells off the Analyzer: Step 3 Cleaning the CFM
1. A pin vise (1) and a 1/64-inch (0.4 mm) diameter drill bit (2) are supplied in the
Flowcell Cleaning Kit. Open the pin vise, insert the drill bit approximately 1/4 inch (6.4 mm) into the vise jaws, then tighten the pin vise.
2. Insert the drill bit into the sheath nipple (3).
Using a twisting motion, carefully push the drill bit through the sheath nipple. If a clog is encountered, proceed slowly while continuing to twist the drill bit.
3. Repeat step 2 for the sample input nipple (4).
4. Carefully insert the bit into the shuttle nipple (5) to a depth that just barely leaves the flutes of the bit extended beyond the end of the nipple. Twist the drill bit a few times, then remove it from the nipple.
Cleaning the Flowcells off the Analyzer: Step 4 Flushing the Flowcell and the CFM
1. Reattach the CFM to the flowcell assembly. Do not overtighten the locking nut.
2. To flush each nipple separately, the other two nipples have to be blocked off. To do this, knot a piece of 0.020(0.5-mm) ID tubing in the middle of its length. Attach the two ends of the tubing to the nipples that are not being flushed.
3. Draw 10 mL of 25%
solution of household bleach and water into the syringe. Connect the syringe and adapter to the flowcell-tube-assembly fitting.
4. Working over a sink, carefully press the plunger to push the contents of the syringe into the flowcell and out the open nipple.
5. Switch one end of the knotted tubing to the cleaned nipple.
6. Disconnect the syringe and the adapter, and draw 10 mL of the appropriate fluid into the syringe. Flush the flowcell with the fluid now exiting through the second nipple.
7. Repeat the flushing for the third nipple
8. Repeat steps 2 through 7 using water to rinse out the flowcell and the CFM.
9. Disconnect the syringe and adapter from flowcell tube assembly fitting.
Cleaning the Flowcells off the Analyzer: Step 5 Cleaning the Glass Windows of the Flowcell
1. Fold a piece of lens tissue so that one edge is slightly narrower than the optical glass window.
CAUTION
To avoid getting material from your hands onto the optical surfaces (glass windows), do not touch the area of lens paper that will come in contact with the glass windows.
2. Soak this edge of the tissue paper with methanol.
CAUTION
Since impure methanol can leave a film on the windows, use only uncontaminated spectrophotometric-grade methanol.
3. Gently wipe one glass surface. Discard the used piece of lens tissue. To prevent formation of a methanol film, completely dry the glass using a clean piece of lens tissue.
4. Examine the clean surface. If the window is not clean enough, repeat steps 1 through 3.
5. Repeat steps 1 through 4 for the other optical window.
Cleaning the Flowcells off the Analyzer: Step 6 Replacing the Flowcell
1. Hold the flowcell by its red threaded fitting and place it gently into the optics assembly.
2. Perox optical assembly
Tighten the release knob to lock the flowcell into position.
RBC/baso/retic optical assembly
Place the flowcell onto the guide pins. Make sure that the flowcell is on both guide pins. The flowcell will be at a 4° angle. Tighten the flowcell release screw.
3. Reconnect the hydraulic lines to the CFM. Reconnect the flowcell tube assembly fitting to the appropriate hydraulic valve (V24 and V23). Hand tighten the connection enough to prevent leaks.
IMPORTANT
To avoid incorrect patient data, make sure that each hydraulic line is attached to the correct CFM nipple.
Shuttle line from UFC assembly (3) Sample line from syringe (2) Sheath line from hydraulic valve (1) V15 - perox or V19 - RBC
Cleaning the Flowcells off the Analyzer: Step 7 Checking Analyzer Performance
IMPORTANT
An inspection scope must be available for this procedure. Do not remove the flowcells unless you are trained in how to align and focus them.
1. Bring the analyzer to the Ready to Run mode.
2. At the Operations menu, select Startup, select Refresh, and verify the background count.
3. Check the flowcell connections for leaks.
4. Check the focus and alignment of the flowcell.
5. Check the gains.
6. Run a whole-blood primer, then run controls to verify analyzer performance.
7. If the control results are acceptable to the laboratory, no additional action is required and normal operation can be resumed.