Time: 6.5 minutes
Analyzer mode: Ready to Run
Perform the system wash procedure at the end of each shift or work period (a maximum of eight hours). After the laboratory shift with the largest number of samples, run three system wash cycles; after other shifts, you need to run only one wash cycle. In addition, if the number of samples in a shift exceeds 400, perform one system wash after the 400th sample.
Example 1: Laboratory workload of 1000 samples/day Shift 1 800 Samples 1 system wash after sample 400
3 system washes at the end of the shift Shift 2 100 Samples 1 system wash at the end of the shift Shift 3 100 Samples 1 system wash at the end of the shift Example 2: Laboratory workload of 300 samples/day
Shift 1 175 Samples 3 system washes at the end of the shift Shift 2 75 Samples 1 system wash at the end of the shift Shift 3 50 Samples 1 system wash at the end of the shift Example 3: Laboratory workload of 350 samples/day
Shift 1 350 Samples 3 system washes at the end of the shift
IMPORTANT
Each EZ KLEEN container (PN T01-3624-54) is sufficient for 20 system wash cycles. Please order your reagents accordingly.
To start a system wash
1. At the Utilities menu, select HydraulicFunctions.
2. Select System Wash, select 1 for the Number of Cycles, then select Start.
Cleaning the Centering Collar
Clean the centering collars and bases if there is residue buildup.
Inspect the autosampler aspirate assembly area for salt buildup. Clean if necessary.
BIOHAZARD
All products or objects that come in contact with human or animal body fluids should be handled, before and after cleaning, as if capable of transmitting infectious diseases. Wear facial protection, gloves, and protective clothing.
The operator should follow the recommendations to prevent the transmission of infectious agents in health-care settings as recommended for potentially
infectious specimens in Protection of Laboratory Workers from Infectious Disease Transmitted by Blood, Body Fluids, and Tissue, 2d edition; Approved Guideline (1997) Document M29-A, National Committee for Clinical Laboratory Standards (NCCLS). This document contains complete information on user protection and it can be used as reference material for instructions on laboratory safety.
Biohazard warning Materials required
• Beakers (2)
• Cotton swab
• Household bleach
• Paper towels
• Stylet or thin wire
• Syringe
• Tubing, 0.020-inch ID
Time: 10 minutes per centering collar Analyzer mode: Off
WARNING
The analyzer must be off; otherwise, personal injury from the needle may occur.
To clean the centering collar
WARNING
To avoid personal injury and exposure to a potential biohazard, you must cover the needle with the red needle cover immediately after you remove the centering collar. Be careful not to bend the needle as you slip the cover over it.
1. Turn off the analyzer.
2. Remove the centering collar from the Autosampler or Manual closed-tube sampler (see specific procedures below).
3. Place the centering collar in a beaker filled with 25% solution of household bleach and water and let it soak for five minutes.
4. Using a cotton swab, scrub off any remaining residue, then rinse with water.
5. Use a stylet or a piece of thin wire to clean the three nipples and the center bore on the autosampler centering collar or the nipple and center bore on the manual closed-tube sampler centering collar.
6. Attach a piece of 0.030-inch ID tubing to a syringe, then flush each port on the autosampler collar or the waste port on the manual closed-tube sampler collar with water.
IMPORTANT
To prevent autosampler centering collar lock ups, apply Parker Super O-lube (or equivalent lubricant) to the barrel part (1) of the centering collar.
Do not get lubricant near the needle port or the needle base (2).
NOTE
The dual Autosampler has two centering collars, one for analyzer sampling and one for optional Autoslide sampling.
Both Centering collars use the same cleaning method.
1 2
7. Reconnect all the tubes, except the sample line on the autosampler, to the centering collar
8. Remove the needle cover and carefully replace the collar over the needle. On the autosampler centering collar, be sure to turn the spring-loaded knob back to its original position.
9. On the autosampler, reposition the autosampler aspirator assembly. Make sure that it drops firmly in place over the guide pins, then reconnect the sample line to the base of the centering collar.
CAUTION
After repositioning the aspirator assembly, finger-tighten the thumb screws, being careful that they are not cross threaded. Overtightening of the screws can warp the baseplate, which will cause misalignment of the sampler. Mis-threading the thumb screws can cause needle damage.
10. Place the tubes going to the autosampler centering collar into the "hook" on the side of the IDee reader.
11. Snap the manual-sampler centering collar into place.
12. Close the analyzer cover.
13. Turn on the analyzer power.
14. Check saline background count and run whole blood primers to verify system performance.
To remove the centering collar from the autosampler
1. Tilt the front cover down.
2. Remove the sample line (1) from the bottom of the needle base.
CAUTION
You must remove the sample line before the aspirator assembly is tilted forward. If the line stays in place, it can break as the assembly is tilted.
3. Loosen the thumb screws (2) and tilt the autosampler aspirator assembly forward.
4. Pull up the spring-loaded knob (3), turn it a ¼ turn, then remove the centering collar (4) by pulling it up and out.
NOTE
If the centering collar is locked into place, squirt a little warm DI water over the collar to loosen it.
5. Place the red needle cover over the needle.
6. Remove the three tubes from the collar.
To remove the centering collar from the manual closed-tube sampler 1. Tilt the front cover down.
2. Pull the centering collar up.
3. Remove the tubing from the nipple.
4. Place the red needle cover over the needle.