Data reduction allows for multiple data calculations of measurement results from the detection methods in the protocol. Raw data results can be
summarized to a single value. Results from the data reduction are used in analysis. Passes allow for additional data reduction using a previous data reduction. Results from the data reduction are displayed in the results and can be used for transformation, concentration, cutoff values, or for validation. The raw data from each detection method is assigned a character that is listed besides the detection method in the Select Methods pane (For example, the first detection method is labeled “A”, the second “B”, and so on). The formula A+B+C would add the raw data from the three detection methods represented by A, B, and C. Detection methods that include multi-point data results can be expanded to view the additional labels (Figure 7-15).
Multipoint methods should have a data reduction (kinetic, area scan,
wavelength scan). If the user does not enter a data reduction, the software creates a standard data reduction once the protocol is saved.
Figure 7-15Data Reduction Page with Labels To configure the data reductions:
1. In Data Reduction Method click Add new to use a data reduction method.
2. Select either the Functions tab or the Formula tab. The Functions tab provides access to predefined data reduction methods. The Formula tab allows for a manually creating a data reduction formula.
Note:The Functions tab is accessible for certain types of detection methods and only on the first pass of the data reduction.
Multi-Mode Analysis Software User Guide
3. If using the Functions tab (Figure 7-16):
Select the desired Basis of Evaluation using the selection box.
As necessary, select a Pre-Read Method. The Pre-Read method will subtract the measurement values of the Pre-Read method from the measurement values of the method selected in Basis of Evaluation.
When necessary, using the Pre-Read Method menu select whether the Pre-Read method will subtract the average for all wells in the Pre-Read method (Mean), or subtract on a well-by well basis. (Well).
Enter a name for the Data using the Name of Data field. This information is used later during data output in the reduced data results.
As necessary, enter any notes about the data reduction using the
Notes field.
In the Reduction Properties section, using the Function field select the predefined data reduction method to use.
As necessary, configure the function’s parameters using the fields displaying below the Function field.
Figure 7-16Functions Tab
Note:Only detection methods that can be used as a basis of evaluation are available for selection.
Note:Each type of read mode has its own set of predefined data reduction functions. See Table A-1 on page 241 for details about the data reduction methods available for sequence measurements.
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OR
4. If using the Formula tab (Figure 7-17):
Enter a formula for the desired data reduction using any of the mathematical operators (except for <, >, or =) or mathematical functions listed in Mathematical Operators and Functions on page 249.
Enter a name for the Data using the Name of Data field. This information is used later during data output in the reduced data results.
Enter the units for the data using the Name of Units field. This information is used for information purposes only during data output.
As necessary, enter any notes about the formula using the Notes
field.
Select the wells to apply the data reduction formula to using the
Calculate Formula for Controls section. Only the selected well types will be used in the current data reduction formula.
Figure 7-17Formula Tab
5. To add an additional data reduction click Add new. OR
To add an additional pass of data reduction formulas click Add new Pass. OR
To add an additional pass of data reduction formulas click Add new Pass.
Note:The Formula field can be used to create ratios, such as A/B, or corrected ratios, such as (A–C)/(B–C).
Note:Up to twenty-six data reductions may be added for each pass. Up to twenty-six passes can be added.
Multi-Mode Analysis Software User Guide
6. Optionally, click the Syntax Helper button, and the Syntax Helper window (Figure 7-18) appears. Syntax Helper helps in the creation of the correct syntax for accessing advanced data values. Access raw
measurement data, blanked data (if blanks are used) or data resulting from any previous data reduction.
Figure 7-18Syntax Helper window
Note:Select the insertion point inside of the formula before starting Syntax Helper.
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Available mathematical functions (Figure 7-19) included in Syntax Helper are minimum, maximum, mean, median, standard deviation or coefficient of variation.
Figure 7-19Syntax Helper mathematical functions selection
Select attributes from the drop-down menus (Figure 7-20) to combine data with mathematical functions and validity constraints to render a desired configuration.
Multi-Mode Analysis Software User Guide
Figure 7-21Syntax Helper parameters
Conclude by clicking on the Insert Syntax button to apply the syntax to the formula at the point of insertion.
7. Add and configure additional passes by repeating steps 2 through 6.
8. Click Next.