DETECTION SYSTEM CONNECTIONS

This section discusses how to connect the UV detector, the Conductivity monitor, the QuadTec detector, and non-Bio-Rad UV detectors.

3.6.1 UV Detector and Conductivity Monitor

Two flow cells are available for use with the UV detector.

• Analytical 5 mm flow cell for high resolution protein chromatography applications and low flow rates.

It has a path length of 5 mm for maximum sensitivity and a volume of only 16 µl. It can be used with flow rates from 0.1 to 10 ml/min.

• Preparative 2 mm flow cell for work not requiring high sensitivity, or when working with high protein concentrations, and for flow rates greater than 10 ml/min. It has a path length of 2 mm and a volume of 30 µl.

Figure 3-8. UV Detector and Conductivity Monitor

To attach the UV Detector and Conductivity Monitor to the rack:

1. Using the information provided above, confirm that the UV flow cell capacity volume is appropriate for the flow rate. Complete discussion of the UV detector and conductivity monitor is provided on pages 2-22 and 2-23, including the procedure for changing the UV flow cell.

2. Using the rod clamps attach the UV detector to a vertical or horizontal bar close to the column outlet.

3. Connect the power cable (square connector) from the UV detector lamp into the connector marked UV Lamp on the rear of the Workstation. Refer to Figures 3-4 and 3-5.

4. Connect the UV detector signal cable (mini-DIN connector) to the connector marked UV Optics on the rear of the Workstation. Refer to Figures 3-4 and 3-5.

5. Connect the Conductivity monitor’s combined power and signal cable (mini-DIN connector) to the connector marked Cond. Flow cell on the rear of the Maximizer, if available. Otherwise, connect it to the Workstation. Refer to Figures 3-4 and 3-5.

6. The Conductivity monitor is designed to be held in the circular notch of the optics bench, but may be placed anywhere in the fluid path.

3.6.2 QuadTec UV/Vis Detector

The QuadTec detector is shipped with a “dummy” flow cell installed. Before operating the detector, you need to install the biocompatible 3mm PEEK flow cell. For complete discussion of the QuadTec detector, including the procedure for installing the flow cell, refer to the QuadTec Instruction Manual.

• Maximizer: If the Maximizer is to be used, the QuadTec should be connected to the Maximizer rear panel Com 1 connector using System Cable 25.

• Workstation: If the Maximizer is not in use, connect the QuadTec via the Instrument Control Module (ICM), as discussed on page 3-11.

Figure 3-9. QuadTec Detector

The ICM translates the signal from the QuadTec detector and transmits it to the DuoFlow Workstation.

Figure 3-10. Instrument Control Module (ICM)

(front and rear views; for use when the Maximizer is not to be used)

INSTR. BUS INSTR. BUS DEVICE

FRONT VIEW REAR VIEW

3

The ICM contains the following:

Front View of ICM Module (required if Maximizer is not in use)

• Address setting: dial should always be set to position 1.

• Instrument bus connector: System Cable 17 connects to the Workstation and the BioFrac fraction collector (if used). (Refer to Figures 3-4 and 3-5.)

Rear View

• Serial Comm connector: System Cable 25 (QuadTec RS232 cable), connects to the QuadTec detector.

• Power connector: System Cable 26, connects to the DuoFlow Workstation DC outlet.

The QuadTec detector is equipped with a universal power supply, which operates with supply voltages from 90 to 260 Volts AC. A manual setting of the supply voltage is not required.

CAUTION! Make sure to use a properly grounded power outlet and the power cable provided with the system.

To connect the QuadTec to the Workstation via the ICM module,

1. Use System Cable 25 to connect from the RS232 connector on the back of the QuadTec to the COMM connector on the ICM module.

2. Use System Cable 26, the ICM power cord, to connect to the back of the Workstation.

3. Use System Cable 17 to connect between an instrument bus connector on the ICM and an available instrument bus connector on the Workstation.

4. If you are using a Bio-Rad device with an instrument bus connector, such as the BioFrac fraction collector, use another System Cable 17 to connect between the instrument bus connectors on the BioFrac and the ICM.

3.6.3 pH Monitor

The pH monitor, available as an option from Bio-Rad, (refer to section 2.5.4 for more information) may be connected to the DuoFlow system in one of two ways:

• To a Workstation connect the Signal Import Module (SIM) included with the pH Monitor. The SIM connects to the DuoFlow Workstation through the bus communication cables (System Cables 17, 18, 19, 21, or 30).

• To a Maximizer, if in use, connect to the rear BNC connector labeled pH.

Figure 3-11. pH Monitor To connect the Bio-Rad pH electrode to the SIM,

1. Connect the output cable from the pH electrode to the connector labeled pH Connector on the SIM.

2. Connect the SIM to the USB Bitbus Communicator by using an Instrument Bus cable (System Cables 17, 18, 19, 21, or 30), as shown below.

Figure 3-12. SIM Connections

WORKSTATION CHART

RECORDER

pH MONITOR CONTROLLER

SIM

SYSTEM CABLE #20

BARE WIRE TO BANANA PLUGS FOR CHANNEL 1 NON-BIO-RAD

UV DETECTOR USB BITBUS

COMMUNICATOR

pH ELECTRODE

pH FLOW CELL SIGNAL CABLE

3.6.4 Non-Bio-Rad Detectors

The Signal Import Module (SIM), available from Bio-Rad, allows you to connect a variety of additional devices such as UV, fluorescence, and RI detectors. Before connecting the detector to the SIM, consult the documentation provided with the detector to determine the cable requirements for connecting to the 3-pin connector (+, -, Gnd) on the SIM.

The SIM connects to the DuoFlow Workstation and Controller through the use of the Instrument Bus cables (System Cables 17, 18, 19, 21, or 30), as shown above. Channel 1 signals are sent to the chart recorder by connecting the UV detector to the chart recorder using bare wires to banana plugs. System Cable 20 transmits start/stop, pen up/down, and event mark signals.