Com 2: Reserved for future use
3. To place runs into the compare dialog, highlight the runs and click the Compare icon on the sidebar
7.2 SETUP SCREEN
The Setup screen is used to select instruments/devices to be used in the protocol for a particular user-defined method. Each method has its own setup user-defined for it. If you routinely use the same setup for different methods, you can select Save setup... from the File drop-down menu to save a setup for use with another method or to define it as the default. This eliminates the need to build a new setup for a method.
In Figure 7-3, Setup screen devices are chosen from the left hand “Available Devices” box. A dialog box appears, allowing you to label the device in the method. For example, an AVR7-3 may be selected and then defined as a Sample Inject Valve. Use the Delete button in the Toolbar to remove a device from the Devices in the Setup window. In order to run your method, these devices must be connected to the rear of the Workstation and they must be powered on.
Note: Devices may not be properly connected to the system but may be included in the setup. The run will not start, however, until the devices are connected. An error message will appear on the Run screen.
Figure 7-3. Setup Screen
The following devices are listed in the Setup screen. Because the UV detector, Conductivity monitor, and AVR7-3 valve are standard with the DuoFlow system, they are always listed in the Devices in the Setup panel. To eliminate any of these, highlight it and click the Delete button.
• Auxiliary Pump: Allows you to use an auxiliary pump, such as the Model EP-1 Econo pump or the Econo Gradient Pump (EGP). See Chapters 2 and 8, for ways to use an auxiliary load pump.
Devices in setup UV Detector
Conductivity Monitor
AVR7-3 Valve - Sample Inject Port 10
Available Devices
Maximizer + Gradient Pump: F10 SVT3-2
Inlet A1: Buffer A1
Inlet A2: Buffer A2
Inlet B1: Buffer B1
Inlet B2: Buffer B2
BioLogic Duo-Flow - <user name> - <project name> - <method name> - <run name>
Edit
Method
New
Method
New
Run Browser Manual Setup Protocol Delete
washload 12
Run Notes
Report PostRun Log Settings
WL1 - 280nm
Flow Rate EGP %B 0 %B
0.00 ml/min 0%
% Split
1.00ml/min 0 %B2
Maximizer + Gradient Pump: F10 UV Conductivity
438 psi 1.003 AU 1.23 mS/cm
QuadTec Econo Gradient
Pump
SIM1/pH SIM1/SIG
0.548 Volt 7.00 pH File Edit View Utilities Options Window Help
Bio-Rad Web
Buffer Blender
Is assigned to buffer blender Is assigned to buffer blender
Is assigned to buffer blender
Is assigned to buffer blender
• Fraction Collector: Selects a fraction collector. The selection options are a BioFrac, Model 2110, Model 2128, or generic (non-Bio-Rad) fraction collector. If you use threshold detection to initiate fraction collection, select either the detector in use or the Conductivity monitor. To enable the use of collection windows or threshold collection for a Model 2110 or generic fraction collector, it is
necessary to assign an SVT3-2 valve as the fraction collector diverter valve.
Selecting the BioFrac or Model 2128 allows you to specify the rack type and number of racks. If the multiple rack option is selected, the system will pause at the appropriate time during the run and a yellow prompt will tell you to change the rack before continuing the run.
The BioFrac and Model 2128 also allow you to specify a delay volume. The Delay Volume function precisely synchronizes fraction collection with the UV signal on the chart recorder. It is defined as the tubing volume between the UV detector and the fraction collector drop-head. If you are using the recommended 1/16” (1.6 mm) OD, 0.020” (0.51 mm) ID Tefzel tubing, 1 cm of tubing has a volume of 2 µl. The volume of the supplied backpressure regulator is 146 µl
• Buffer Blender: There are a number of buffer systems for use with DuoFlow Maximizer systems.
From the Buffer System window, select the desired buffer system. As a buffer system is selected, the pH range at 25° C, molarity at 100% B, temperature compensation coefficient, and recipe are displayed in lower boxes. Indicate if a pH correction is to be used, either single or two point. Buffer recipes may be printed by selecting Print Report from the File menu or the Report toolbar button.
The selection includes a method option.
• Detectors: The UV detector and Conductivity monitor are provided with DuoFlow systems. The QuadTec detector from Bio-Rad is discussed in greater detail in its instruction manual. SIM/pH is for use with Bio-Rad’s pH monitor and SIM (Signal Import Module). SIM Signal is for use with non-Bio-Rad detectors, such as refractive index and fluorescence detectors.
The SIM Signal button requires that you set the Units and the minimum and maximum for the Units Range and Device Output Range (volts). Select the device number on the SIM to match the number displayed. Refer to the analog device’s documentation for information on output voltages.
• Valves (SVT3-2, SV5-4, AVR7-3, and AVR9-8): The DuoFlow system’s automatic valves can be configured in a number of different ways depending on their function in a method. Table 7-1 discusses how each of the valves can be set up.
• Gradient Pump: Inlets A and B may be renamed by overwriting “Buffer A” and “Buffer B” with the description of the buffer. When the Maximizer is used, the inlets become A1, A2, B1, and B2.
Valve Function and Naming
One of the most powerful features of the DuoFlow Software is the ability to define the specific purpose of the automated valves and to name each valve position. This greatly simplifies programming individual steps in the Protocol screen.
For example:
• The most common use of an AVR7-3 valve is as a sample inject valve. Once an AVR7-3 valve has been assigned to this function, the software controls all of its valve changes based on the
programmed sample injection step.
• The most common use of a SVT3-2 valve will be as a fraction collector diverter valve with a Model 2110 or generic collector. Once an SVT3-2 valve has been assigned to this function, the software controls eluant diversion based on the programmed fraction collection scheme.
In summary, you can identify the following as applying to all valves:
• Valve Name/Function. What is the valve to be used for? Select from the drop-down list box or input a unique function that you will recognize when the particular valve is selected in the Protocol screen.
• Connector. Which connector at the rear of the workstation is the valve connected to?
Table 7-1.
Valve Setup Information
Notes
Functions as a fraction collection diverter, determined by the actual fraction collection parameters chosen.
When used before the inlet to Pump A or B, the valve enables buffer selection. The buffer name specified for each position will appear in the Protocol screen’s “Isocratic Flow”, “Linear Gradient”, and “Change Valve” dialog box. Refer to Chapter 8, Sample Loading, for examples.
Used for auxiliary pump load selection to select one of two solutions. Refer to Chapter 8, Sample Loading, for examples.
When used for a purpose other than described above. The name specified for each position will appear in the Protocol screen’s “Change Valve”
dialog box.
When used before the inlet to Pump A or B, the valve provides preparative sample loading or buffer selection. The buffer or sample name specified for each position will appear in the Protocol screen’s “Isocratic Flow”, “Linear Gradient”, and “Change Valve” dialog box. Refer to Chapter 8, Sample Loading, for examples.
Used for auxiliary pump load selection to select one of four solutions. Refer to Chapter 8, Sample Loading, for examples.
When used for a purpose other than that described above. The name specified for each position will appear in the Protocol screen’s
“Change Valve” dialog box.
For automatically loading a sample.
When used for a purpose other than described above. The name specified for each position will appear in the Protocol screen’s “Change Valve”
dialog box. Refer to Section 4, Advanced System Valve Type Valve Name/ Position
Function Names
SVT3-2 Fraction Collector 1 Waste Low pressure Diverter 2 Collect solenoid valve
Note: Indicate Inlet A Named by user the location
of the valve Inlet B Named by user
cable connection.
When the
Maximizer is Aux Pump Inlet Named by user used, inlets A
and B become A1, A2, B1, B2.
User Assigned Named by user Name
SV5-4 Inlet A Named by user
Low pressure
solenoid valve Inlet B Named by user Note: Indicate
the location of the valve cable
connection. Aux Pump Inlet Named by user When the
Maximizer is
used, inlets A User Assigned Named by user and B become Name
A1, A2, B1, B2.
AVR7-3 Sample Inject 1 Load Sample
High pressure 2 Inject Sample
valve 3 Purge
Note: Indicate User Assigned Named by user the location Name
of the valve cable connection
Table 7-1. (continued) Valve Setup Information
Valve Type Valve Name/ Position
Function Names
AVR9-8 Aux Pump Inlet Named by user High pressure
valve
Note: Indicate Inlet A Named by user the location
of the valve Inlet B Named by user
cable connection.
When the Maximizer is used, inlets A
and B become Fraction Collector 1 Waste
A1, A2, B1, B2. 2 Collect
3-8 Named by user User Assigned Named by user Name
Column Switching Named by user
Notes
Used for auxiliary pump load selection to select from up to eight samples, buffers, or rinse solution.
When used before the inlet to Pump A or B, the valve provides preparative sample loading or buffer selection. The buffer or sample name specified for each position will appear in the Protocol screen’s “Isocratic Flow”, “Linear Gradient”, and “Change Valve” dialog box. Refer to Section 4, Advanced System Applications, Chapters 8 and 9.
Useful for collecting large volume samples.
Collects up to 8 samples. The name specified for each position will appear in the Protocol screen’s
“Change Valve” dialog box.
When used for purposes other than described above. The name specified for each position will appear in the Protocol screen’s “Change Valve”
dialog box. Refer to Chapters 8 and 9.
Allows you to install up to 8 columns and assign valve numbers and names to each column.
Requires two AVR9-8 valves: one is an inlet valve and the other functions as an outlet valve.