Evaluation Functions - VisiSens AnalytiCal 2 Software

Button Description

Z-axis profile: Calculates and shows the graph of the average values of all uploaded images in a user defined ROI (region of interest).

Live profiles: Shows the x and y representation of the current row in the loaded image. Move the mouse over the image to get live

representation of the respective row.

ROI statistics: Mark a ROI (region of interest) and get statistics displayed.

Follow kinetics: Shows the distance from the first to the last of the uploaded images.

Pseudocolor image: Displays the pseudocolor image of the currently shown measurement image.

Histogram: Displays a histogram representation of the currently shown measurement image.

3D plot: Shows a 3D representation of the measured values on the currently displayed image.

Multiple plots: Multiple representations of the measured values on the currently displayed image are shown in one diagram.

VisiSens AnalytiCal 2 Evaluation Module

5.4.1 Z-Axis Profiles

Z-axis profiles will calculate and display a graph of average values in a certain ROI (region of interest) for all images uploaded.

Click the button. Now you are able to mark a ROI with left mouse clicks. Close the ROI polygon with a right mouse click. The software will calculate average values in the ROI for all images and display the respective graph (see Fig. 53).

A dialog opens asking if the calculated data should be saved. Clicking Yes will save the graphical representation as .jpg and the data as .dat file. Pressing No will close the window without saving the data.

Fig. 53 Z-axis profile – graphical representation

VisiSens AnalytiCal 2 Evaluation Module

The .dat file gives information about average values of the area, the number of pixel used for evaluation, the number of valid pixel in the marked area, standard deviation, maximum and minimum values.

5.4.2 Live Profiles

Pressing the button opens the Profiles window that shows x and y representations of the current row in the loaded image. By moving the mouse over the image you can get live profiles for the respective row. Double clicking the image while the Profiles window is open, will switch x and y axis of the representation.

A right mouse click will close the Profiles window.

VisiSens AnalytiCal 2 Evaluation Module

5.4.3 ROI Statistics

Clicking the button you can select a ROI on the image. Use left mouse clicks to create a polygon and a right mouse click to close it. The software will calculate and show statistical values for the selected ROI (average, and standard deviation are also shown in the colorbar):

1. (Avg.) average value

2. (max) maximum value

3. (min) minimum value

4. (St. Dev.) standard deviation

5. (valid pixel) the number of valid pixel inside the ROI: the Raw-Values of the pixels are within the valid range for pH calculation, if they are not within the valid range the respective pixels are set to value of x pH and not accounted for in the calculation.

6. (pixel) the total number of pixel inside the ROI

VisiSens AnalytiCal 2 Evaluation Module

5.4.4 Follow Kinetics

Use the button to get a graphical representation of a distances from the first image to the last image. With this function you are able to investigate the spatial change of an area of interest over time (e. g. the border of an area of low pH that spreads or shifts over time). Press the button and click on the area of interest on the first image. The software will

automatically switch to the next image, click again. Proceed this way until you have clicked on all uploaded images always marking the area you are interested in. The graphical

representation will be shown after clicking the last image giving you the information of distance from origin in pixel.

5.4.5 Pseudocolor Image

Pressing the button will show the pseudocolor image of the currently displayed image.

VisiSens AnalytiCal 2 Evaluation Module

5.4.6 Histogram

Use the button to view the histogram representation for the currently displayed image.

5.4.7 3D Plot

The button will enable you to get a 3D plot of the measurement values in the currently displayed image.

Fig. 58 Histogram

Fig. 59 3D Plot of pH buffer drop recording – high values in the center can be clearly distinguished from lower levels towards the edge

VisiSens AnalytiCal 2 Evaluation Module

5.4.8 Multiple Plots

Get a graph with multiple representations of the measurement values in the currently displayed picture by pressing the button.

VisiSens AnalytiCal 2 Concluding Remarks

6 Concluding Remarks

Dear Customer,

With this manual, we hope to provide you with an introduction to work with the VisiSens AnalytiCal 2 software.

This manual does not claim to be complete. We are endeavored to improve and supplement this version.

We are looking forward to your critical review and to any suggestions you may have. You can find the latest version at www.PreSens.de.

With best regards, Your PreSens Team

© 2014 PreSens Precision Sensing GmbH Manufacturer PreSens Precision Sensing GmbH Josef-Engert-Straße 11 93053 Regensburg Germany Phone +49 941 94272100 Fax +49 941 94272111 [email protected] www.PreSens.de

In document VisiSens AnalytiCal 2 Software (Page 44-52)