Consensus initiator ^ P_P_CA TP_P_P.Y y y y
6.8 Down-regulation of annexin
In response to PM A/A23187, annexin VI was shown to be down-regulated in a T-cell specific manner. Although the protein level dropped after 12 hours, it was clear that the mRNA level declined much more rapidly (between zero and four hours). This was a pronounced down-regulation and was specific for annexin VI (annexin I was
unaffected). W hile it is not certain that the down-regulation was a direct result of LHR- protein binding, it is highly likely that the two events are linked.
Why annexin VI should be down-regulated in response to these agonists is not clear. Dubois et al, (1995) have shown that in Jurkat cells, annexin VI interacts with the protein tyrosine-kinase p561ck which is a component of the T-cell receptor signalling pathway. Although the effects of this interaction are unknown, annexin VI may
influence the signalling events downstream of Ick. However, this does not shed light on the mechanism o f annexin VI down-regulation. A clue may lie in the work of Clark et al (1991), showing that in maturing T-lymphocytes, annexin VI levels increase. In this study, individual T-cell subtypes were not analysed, so it is not known whether this was restricted to T h l or Th2 cells. However, this is consistent with modulation of annexin VI levels being an important feature of T-cell development. The answers to these questions will be found with the generation of mice lacking the annexin VI gene. If these show altered T-lymphocyte function or development, then this would confirm the importance o f annexin VI in these cells.
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