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A simple breathing circuit allowing precise control of inspiratory gases for experimental respiratory manipulations

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Figure

Figure 1 Schematic comparison between the new breathing circuit and a non-rebreathing oxygen mask
Figure 2 Schematic of the circuit assembly.of two different types, with a luer-lock or barbed sampling port (TM #1624 or #1632 respectively)
Figure 3 Respiratory traces of hyperoxic manipulations using the new circuit (right) vs
Figure 4 Reproducibility of O2 and CO2 manipulations using the new breathing circuit. Manipulations consisted of two runs of the 18-minuteschedule described in ref [7]

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