• No results found

Multiplication of parvovirus LuIII in a synchronized culture system. III. Replication of viral DNA.

N/A
N/A
Protected

Academic year: 2019

Share "Multiplication of parvovirus LuIII in a synchronized culture system. III. Replication of viral DNA."

Copied!
13
0
0

Loading.... (view fulltext now)

Full text

Figure

FIG. 1.pulseMofsynchronizationoflarinfectionmock-infectedwerelar-weightmonolayer [3H]TdR Tris-O.001 Incorporation of ['H]TdR into low-molecu- DNA in both LuIII virus-infected (0) and (0) synchronized HeLa cells
FIG. 2.pHLuIlfDNAthe Sedimentation analysis of low-molecular-weight DNA extracted from both mock-infected (0) and virus-infected (0) synchronized HeLa cells at various times p.i
FIG. 3.DNA Sedimentation analysis of double-labeled of low molecular weight extracted from (A)
FIG. 4.nucleaseculturesconditions.Ci/mmol)(dsDNAdialysissingle-strand-specific Fractionation by sedimentation in neutral CsCl (A) and digestion with the single-strand-specific Si (B) of Hirt supernatant DNA extracted from LuIlI virus-infected HeLa cells
+4

References

Related documents

By investigating the spatial distribution of IE2 in the nuclei of AcMNPV-infected cells with respect to the sites of viral DNA synthesis, we demonstrated that IE2 structures

We have studied the DNA replicative interme- diates in cells infected with a dar mutant and a dar-amC5 (gene 59) mutant by velocity sedimentation in neutral and alkaline

Second, the right end, which is near the origin for RF DNA replication and probably progeny DNA synthesis as well, contains the 5'-phosphoryl terminus of the viral strand (15, 17,

process of asymmetric single-stranded viral DNA synthesis is blocked at the late period of these mutant infections, an asymmetric synthesis of the viral strand of replicative-form

Cell DNA synthesis in Py-infected WT-4 and ts AlS9 cells: size analysis by velocity sedimentation in alkaline sucrose density gradients.. It has been demonstrated

Thermolabile DNA-binding proteins from cells infected with a temperature-sensitive mu- tant of adenovirus defective in viral DNA synthesis.

Analysis by alkaline sucrose gradient cen- trifugation of viral DNA labeled in Ad2-infected HeLa cells in the presence of HU. (Upper

In one experiment an alkaline sucrose density gradient was "under-loaded" with approximately 8,000 labeled, Ad2-infected cells (about 0.14 ug of DNA). Upon zonal