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Expression and extracellular release of human immunodeficiency virus type 1 Gag precursors by recombinant baculovirus-infected cells.

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FIG. 1.myr,drinandsequence375inpolyhedrinforeignrestriction the Polypeptide domains (p17MA, p24CA, p9NC, and p6LI) N- and C-terminal sequences of recombinant Gag molecules
FIG. 3.WTprecursorlularrespectively.moleculesusingallydifferentdardBarsdeterminedtively Quantitative assays of intracytoplasmic (a) and extracel- (b) Gag molecules recovered from uninfected (UI), AcNPV- (WT)-infected, or AcNPVgagrecombinant-infectedcellsat
FIG. 0) Gagl2myr Polypeptide (lane labeling 12), or of Gag5 5). Cells were labeled or infected (lane AcNPV-WT (lane WT), Gagl4myr (lane 13),__ cells mock ~30 Sf9 infected (lane_ x_ 4
FIG. 5.gradient,Viruslikefluorographyrepresentsconditions,ofduepanelaremolecular600SnucleocapsidskilodaltonGagGagl2myrtrifuged(180labeledand adenovirus Velocity gradient centrifugation analysis of extracellular released from cells infected by Gagl3myr (a),

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