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Disruption of the G1/S Transition in Human Papillomavirus Type 16 E7-Expressing Human Cells Is Associated with Altered Regulation of Cyclin E

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FIG. 1. Cyclin and CDK protein levels in asynchronous and Gor G0/G1-arrested cells. Total-cell lysates were prepared from asynchronous HFKs and HFFs (A and B)/G-arrested fibroblasts (C) expressing the designated viral gene
FIG. 5. Cell cycle arrest following serum starvation. HPV-16 E7- and vector-expressing HFFs were starved of serum for 48 h, restimulated with 10% FBS, andmonitored at intervals for 30 h after release
FIG. 7. The CR1 and CR2 domains of E7 are required for increased cyclinE levels. Total-cell lysates were prepared from asynchronous HFK expressing the

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