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Role of cullin elonginB elonginC E3 complex in bovine immunodeficiency virus and maedi visna virus Vif mediated degradation of host A3Z2 Z3 proteins

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Figure 1 BIV/MVV Vif affect the synthesis rate of btA3Z2-Z3/oaA3Z2-Z3, respectively, via a proteasomal pathway
Table 1 Homology rates of EloB, EloC, Cul2, Cul5 andCBF-β between humans, cattle and sheep
Figure 2 BIV Vif recruits Cul2-EloB-EloC and MVV Vif recruits Cul5-EloB-EloC to induce btA3Z2-Z3 and oaA3Z2-Z3 degradation.(A, B) 293 Tcells (5 × 106) were transfected with 10 μg HA-tagged BIV Vif or 10 μg VHL and 10 μg VR1012 as a positive and a negative
Figure 3 BIV Vif interacts with btCul2 and MVV Vif with oaCul5, while CBF-anti-cmyc antibody, followed by SDS-PAGE and immunoblot analysis using anti-cmyc and anti-Flag antibodies.BIV Vif (MVV Vif or HIV Vif) and 5β are not involved
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