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Human T-Cell Leukemia Virus Type 2 Rex Protein Increases Stability and Promotes Nuclear to Cytoplasmic Transport of gag/pol and env RNAs

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Academic year: 2019

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Figure

TABLE 1. Analysis of Rex function by HTLV Gag protein ELISAa
FIG. 2. Oligonucleotide primer pairs for RT-PCR using HTLV-2 RNAs. (A) Schematic representation of the HTLV genome showing locations and orientationsof the oligonucleotides used for PCR
FIG. 5. Rex increases the stability of unspliced gag/polto amplify full-lengthvarious times (0, 1, 3, 6, 12, and 20 h) following incubation with actinomycin D (5primer extension–25-cycle PCR in the presence (of wtHTLV-2 or 25 RNA in transfected JM4 T cells
FIG. 6. Effect of Rex on nuclear and cytoplasmic RNA distribution. JM4 human T cells (107expression vector); 48 h posttransfection, 10of the cells

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