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Pseudorabies Virus UL3 Gene Codes for a Nuclear Protein Which Is Dispensable for Viral Replication

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Figure

FIG. 1. Diagram of the UL3 coding region and construction of PrV-�Bamare transcribed into 3upward)
FIG. 2. Identification of the PrV UL3 protein. (A) Purified PrV-Ka and PrV-�((L) were analyzed by Western blotting with monospecific sera against the UL3 (�-gH) or a monoclonal antibody against gB (UL3F2 virions (V) and the respective infected cell lysates�-U
FIG. 5. Intracellular localization of the PrV UL3 protein. RK13 cells were infected under plaque assay conditions with PrV-Ka, PrV-�PrV-with the monospecific anti-UL3 serum and Alexa 488-conjugated secondary antibodies (green)
FIG. 6. Growth properties of PrV-�harvested immediately or 4, 8, 12, 24, and 36 h p.i
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