P o s t n o v a A n a l y t i c s – I n v e n t o r s o f F F F
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The Most Powerful Temperature Controlled
Asymmetric Flow FFF Separation Technology
P o s t n o v a A n a l y t i c s – I n v e n t o r s o f F F F S S p p o o s stt n noo v vaa aannaa l lyy tt iic c ss ● ● ●● I INN C CEE11998866 20 20 YEARS YEARS S S p p o o s stt n noo v vaa aannaa l lyy tt iic c ss ● ● ●● I INN C CEE11998866 20 20 YEARS YEARS
POSTNOVA ANALYTICS
Postnova Analytics is completely focused on Field-Flow Fractionation (FFF) and Light Scattering (LS) technology, offering a unique product portfolio of integrated FFF and LS systems. Postnova combines the heritage of Prof. Giddings, the inventor of FFF, with a unique 40 year long history in FFF science. The company has an unlimited commitment to Field-Flow Fractionation and Light Scattering excellence and always striving to represent the center of excellence in FFF and LS business. Our customers can expect the most advanced and reliable analytical solutions for their demanding applications from nanotechnology, polymers and bioscience.
FFF HISTORY
In 1966 Field-Flow Fractionation was invented by Prof. Calvin Giddings (1930-1996) [01], an outstanding scientist and two-times Nobel-Price nominee (1984/1994), from University Utah at Salt Lake City, USA. He also was the founder of the FFFresearch Center at University of Utah, where he and his team developed the complete theory of FFF and the different sub-techniques, such as Thermal FFF [02] in 1969, Sedimentation FFF [03] in 1974, Flow
FFF [04] in 1976 and SPLITT in 1985 [05]. In 1987 Giddings/Wahlund published the first Asymmetric Flow FFF [06], which later became the most popular FFF technology used today. 1984 Prof. Michel Martin from Univ. Paris published the world’s first online coupling of FFF with Light Scattering [7,8]. In 1986, Prof. Giddings and his co-workers founded the legendary FFFractionation Inc. in Salt Lake City, which was the first FFF company ever. FFFractionation developed and introduced the first commercial FFF instruments, which were the Model T100 Thermal FFF, S101 Sedimentation FFF, F1000 Flow FFF and
SF1000 Split Thin Flow Cell Fractionation (SPLITT) [9,10,11,12]. In 1995 Dr. T. Klein developed a new type of Asymmetric Flow FFF after studies at Ciba-Geigy (now Novartis), in Basel, Switzerland and the Technical University of Munich, Germany. After that he founded Postnova Analytics in Munich in 1997, which introduced the first Asymmetrical Flow FFF into the market. In 2001 Postnova Analytics and FFFractionation merged together, forming the leading company in the area of FFF LS. Just recently, Postnova Analytics presented the world’s first High Temperature [13] and Mid Temperature Asymmetric Flow FFF technology.
Literature:
[1] J.C. Giddings, “New separation concept based on a coupling of concentration and flow non-uniformities”, Separation Sci., 1 123-125 (1966). [2] G.H. Thompson, M.N. Myers, and J.C. Giddings, "Thermal Field-Flow Fractionation of Polystyrene Samples", Anal. Chem., 41 1219-1222 (1969). [3] J.C. Giddings, F.J.F. Yang, M.N. Myers, "Sedimentation Field-Flow Fractionation", Anal. Chem., 46 1917-1924 (1974).
[4] J.C. Giddings, F.J. Yang, M.N. Myers, "Flow Field-Flow Fractionation: A Versatile New Separation Method", Science, 193 1244-2145 (1976). [5] J.C. Giddings, "A System Based on Split-Flow Lateral-Transport Thin (SPLITT) Separation Cells for Rapid and Continuous Particle Fractionation", Sep. Sci. Technol., 20 749-768 (1985).
[6] Wahlund, K.G.; Giddings, J.C.; “Properties of an Asym. Flow FFF channel having one permeable wall”, Anal. Chem., 59 1332-39 (1987). [7] Martin, M.; “Polymer analysis by fractionation with on-line light scattering detectors”, Sep. Sci. Technol.; Sep. Sci. Technol.; 19 685-707 (1982). [8] Martin, M.; Hes, J.; “On-line coupling of thermal field-flow fractionation with laser light scattering”, Chromatographia; 15 426-432 (1984). [9-12] FFFractionation, Inc., Salt Lake City, Utah, USA “Model F1000”, “Model S101”, “Model T100”, Model “ SF1000”, Product Brochures, (1986). [13] H. DeJonge, E. Mes, T. Klein, R. Welz, “High Molecular Weight Polyolefin Analysis by High Temperature Asymmetrical Flow Field-Flow Fractionation”, Oral Presentation/Poster 1st
ICPC Conference, Houston, USA, Oct. 15th -18th
P o s t n o v a A n a l y t i c s – I n v e n t o r s o f F F F
1986 1997 2004 2008
FFF PRINCIPLE
Field-Flow Fractionation is a family of unique separation techniques, comprising of various different sub-techniques, just like chromatography. All FFF versions utilize the same basic separation principle, but employ different separation fields. Depending on the used separation field, the technique is called Flow FFF, Sedimentation FFF, Thermal FFF, etc..
FFF is providing fast, gentle and high resolution separations of any particulate matter from 1 nm up to 100 µm in any liquid media. The sample is separated inside an open flow channel without the presence of any packing or stationary phase inside. Inside the channel a laminar flow with a parabolic stream profile is formed.
The different used force fields, such as liquid flow, centrifugal force, temperature gradient or gravity, are applied perpendicular to the main flow which transports the sample through the channel. Under the influence of these force fields and the counteracting diffusion of the particles, different equilibrium layer heights are formed by the different particle size fractions. Smaller particles, with stronger diffusion are located higher in the channel in fast stream lines and elute first. Bigger particles with a lower diffusion coefficient are located in slow stream lines and elute later.
ASYMMETRIC FLOW FFF
Flow FFF has been the most important FFF technique since two decades now, whereas Asymmetric Flow FFF is subversion of Flow FFF. Postnova Analytics has been setting the standard in this area since the beginning. The company developed and introduced the first Flow FFF instrument(Model F1000) in 1986, launched the first commercial automated Asymmetric Flow FFF (Model HRFFF-10.000) in 1997, first integrated Asym. Flow FFF in 2004 and finally invented and presented the first temperature controlled Mid Temperature Asymmetric Flow FFF in 2007. Today, Postnova’s offers its customers the most advanced FFF technology, protected by and based on several patents.
In Asymmetric Flow FFF the separation force is generated by a flow field (cross flow) inside the channel. The cross flow is divided from the main flow pumped through the channel and is directed through a semi-permeable membrane which is located at the bottom wall. The membrane pores size prevents the sample to pass through, but allows the solvent to exit the channel easily. The sample fractions are eluted out of the channel in direction to the detectors by the remaining channel flow.
P o s t n o v a A n a l y t i c s – I n v e n t o r s o f F F F
AF2000 MT – MID TEMPERATURE ASYMMETRIC FLOW FFF
Postnova has invented a new type of temperature controlled Asymmetric Flow FFF. Actually it is the first commercial Flow FFF instrument available with temperature control for advanced FFF studies. The system outperforms GPC, because of its huge separation range, high separation power and ability to separate proteins, polymers and nanoparticles at the same time. The AF2000 MT is setting a complete new standard in the characterization of macromolecules and marks a real break-through in FFF science. The system is using Postnova’s patented channel cartridge design, which enables the user to access complete new applications and to overcome the limitations of the traditionally used other systems.
UNIQUE FEATURES OF AF2000 MT
Channel CartridgeThe channel cartridges used in the AF2000 series system are based on Postnova’s patented technology. Micro and analytical channels are available and can be exchanged easily by just replacing the cartridge.
Broad Separation Range
Other than as in HTGPC, the separation in MTFFF can also be performed for polymers up to molar masses of 10E9 Da. No size exclusion effect is observed, making MTFFF the ideal tool for the separation of large and ultra-large polymers or even nanoparticles.
Gentle Separation Conditions
Because of the open channel and the absence of any stationary phase the separation can be performed under the absence of shear forces and stress to the macromolecule. Typical HTGPC problems, such as filtering/adsorption effects, shear induced chain degradation and late elution effects can be avoided.
Patented FOCUS Technology
The AF2000 MT incorporates Postnova’s patented FOCUS technology, to ensure a continuous and stable detector flow at any time. This is an absolute necessity, to be able to connect flow sensitive viscometers and RI detectors. Additionally to this, the technology provides also a higher sample recovery, higher resolution and a higher sensitivity. Another striking advantage of the Focus technology is that the channel outlet can be directly connected to the detector without any valve or T-piece build in between. This ensures short transfer line, low dead volumes and minimized band broadening.
Patented Cross Flow System
Postnova is using a patented technology for generating the cross flow inside the FFF channel, based on a double syringe pump. This pump is controlled in a special unique way avoiding any pulsation. The pump is completely inert and can easily produce constant and absolute flows ranging from 0,005 to 10 ml/min for different solvents. Using the volumetric based syringe pump technology, a certain volume per time is removed independent from the nature of the solvent and the temperature.
NovaFFF Software Platform
Postnova offers an integrated single software platform for system control, data acquisition and data evaluation of different FFF and detector systems. The software incorporates an advanced Light Scattering module for molar mass and particle size calculations.
Flexibility & Interfaceability
The AF2000 MT series was developed to be used as Stand-Alone system or interfaced with existing HTGPC systems from various manufacturers. This gives the advantage that FFF and GPC can be performed using the same set or detectors, using the same temperature chamber for GPC and FFF.
P o s t n o v a A n a l y t i c s – I n v e n t o r s o f F F F
NOVAFFF SOFTWARE
The NovaFFF software is based on a single platform strategy, incorporating system control, data collection and data evaluation as well as reporting.
Set-up & Handling & Security
The NovaFFF software is easy to use and incorporates the complete knowledge of over two decades in FFF and Light Scattering technology. It is very flexible, as different hardware components, such as autosampler, pumps and various detectors can be selected by the user with just a few mouse clicks. Various security and monitoring functions have been built into the software. The pump and channel pressure is actively monitored. The system can be shut down manually or automatically when a set maximum
pressure value is exceeded. The main actions and controls done by the software are stored in a log history file. Also, all important data such as pressures, flows and additional analog detector signals are collected and stored with the run data.
Modern System Control
State-of-the-art modern communication protocols are standard in this software solution. The communication of the program with PC and the system components is achieved via TCP/IP Ethernet protocol and no data acquisition boards or external controllers are necessary. As the FFF system has it’s own IP address, thus the complete system can be operated via the local network in the user’s environment.
Seamless FFF-LS Integration
The software uniquely combines the system control of FFF and the selected Light Scattering detector in just one application. Start-up of two programs, copy and pasting of methods between the different programs or problems about the stability of the multitasking capabilities of the PC operating system are history when using NovaFFF.
Data Evaluation and Calculation
After finishing a sample measurement, immediately the data evaluation, molar mass and radius calculations can be performed. Also the corresponding mass and radius distributions, conformation plots and different reporting functions can be accessed, while the system is performing the next run. Unique Features at a Glance
One software platform for different FFF techniques
Single program for system control, data acquisition and evaluation Direct control and data acquisition of different light scattering detectors Easy to use software with fast start up procedure using run/method templates Highly flexible program, as different hardware can be selected
Advanced Light Scattering data evaluation and reporting module incorporated Various built-in manual and automatic security options for safe and smooth operation
P o s t n o v a A n a l y t i c s – I n v e n t o r s o f F F F
SPECIFICATIONS
Temperature Range: Cooling real 5°C Heating max. 80°C Measurement Range:Polymers: 104-109Da / Nanoparticles: 1nm-10µm (Depending on membrane and sample material) Channel Cartridge System:
Patented Postnova channel system:
- Inner Volume: 0,5 -1,5 mL depending on spacer - Inner Channel Thickness: 350 µm (others available) - Outer Dimensions: 335 x 60 x 40 mm
Applicable Solvent Systems:
All typical organic and aqueous solvents. Connected Detector Systems:
The same well-known detection technologies which are used in HTGPC can also be used in HTFFF: - Low/Right/Multi Angle Light Scattering for MW/Rg
- Viscometric Detection for MW/Rh - Refractive Index for Concentration
- Infrared Detection for Polyolefin Concentration - Other Technologies: Please inquire on availability Flow Rate Ranges / Pressure:
Detector Flow 0,01-2,0 mL/min at channel outlet Cross Flow 0 or 0,005-10 mL/min
Channel Pressure up to 50 bar maximum Required Analysis Time:
Typically 10-20 min; shorter/longer times possible Electrical Power Requirements:
230V, 50Hz: Min-Max / 1,2 kW-3,6kW at 6,3-15,6A 110V, 60Hz: Min-Max / 0,6 kW-1,8kW at 6,3-15,6A 100V (Japan): Please inquire for power requirements. All values are for the basic set-up without detectors only. PC Requirements:
Windows OS, min.1024 MB RAM, 2 Ethernet ports Software System:
Unique NovaFFF software platform for complete system control (autosampler, pumps, detectors), data acquisition (detector signals, pressure, flows) and evaluation as well as reporting functions (static light scattering calculations, standard calibration). Maintenance Parts:
Pump seals, pistons and check valves as usual. The channel cartridge itself is inert and made of special unique materials. It can be regenerated after heavy use and thus be re-used many times.
Injection Volume:
- Typically 10-1000 µL with autosampler injection / - Typically 10-10.000 µL with manual injection Band broadening is not affected by injection volume because of patented focus technology used inside. Injected Sample Mass:
Limiting parameter is channel overloading, which is primarily depending on the channel dimensions. Typical injected sample mass for given channel size is 10-100 µg. The maximum sample mass can go up to 500 µg, depending on sample nature.
P o s t n o v a A n a l y t i c s – I n v e n t o r s o f F F F
FFF & LS PRODUCTS
Postnova Analytics is focused on products based on Field-Flow Fractionation and Light Scattering for applications in Biotechnology, Pharma, Chemistry and Environmental Science. We offer complete analytical systems, services and supplies from one hand.
AF2000 AT/MT/HT Series – Flow FFF
The most advanced Flow FFF technology for the characterization of proteins, polymers and nanoparticles in aqueous and organic solvents at ambient (AT), middle (MT) and high temperatures (HT). Unbeaten reproducibility and resolution can be achieved easily with these kind of Flow FFF series.
S101 Series – Sedimentation FFF
The benchmark in high resolution FFF separation of nanoparticles, such as emulsions, latexes and colloids in aqueous solvents. Model S101 is ideal for biological particles as well and can be applied in the range from 50nm to 100 µm.
TF200 Series – Thermal FFF
Our brand new TF2000 Series is based on the Thermal FFF principle and is the technology of choice for characterization of polymers and gels in organic solvents. Thermal FFF can separate polymers by molar mass and by chemical composition.
PN3000 Series – Static-Dynamic Light Scattering (MALS/DLS)
The PN3000 MALS series is the ideal LS detector for coupling with FFF, GPC and HPLC. The compact instrument is available as Single/Dual/Multi-Angle Static LS version for ambient and high temperature applications. Dynamic Light Scattering (DLS) as an extra option is available as well.
Worldwide Class Support
Postnova Analytics is located where its customers are located. Beside our facilities in Germany (HQs) and America (USA), we have established a local support network of highly qualified partner organizations. If you just need a spare part, if you are looking for a special training course, or if you need immediate help for a demanding application, Postnova can assist you like no other in the FFF - LS business.
EAC – European Application Center AAC – American Application Center
The European and the American Application Centers (EAC / AAC) which are located in our German and US offices are playing a central role in our
support activities. These centers provide the highest quality application support to the scientific community and host the training and educational services, which are offered for all our product lines on a frequent basis, as well. The long history in FFF and the broad FFF product range are building the basis for this unsurpassed, extensive scientific support offered. This is underlined by the significant number of former scientists and PhDs coming from Prof. Giddings group, which have been employed by FFFractionation/Postnova over the years.
CONTACT
Postnova Analytics GmbH, Max-Planck-Str. 14, 86899 Landsberg, GER, T:+49 8191 428 181, F:+49 8191 428 175 Postnova Analytics, Inc., 230 South, 500 East, S. 120, 84102 Salt Lake City, USA, T: +1 801 521 2004, F:+1 801 521 2884 Email: [email protected] - Web: www.postnova.com
Postnova Analytics GmbH (Europe,Asia,ROW) • Max-Planck-Str.14 • 86899 Landsberg, Germany Postnova Analytics Inc. (The Americas) • 230 South 500 East Suite 120 • Salt Lake City/UT, USA
[email protected] • www.postnova.com
Postnova Product Portfolio
Fax: +49 8191 428-175 Tel: +49 8191 428-181 www.postnova.com
Field-Flow Fractionation Systems for Protein, Particle and Polymer Separation & Characterization
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AF2000 AT: Asymmetric Flow Field-Flow Fractionation for Particle, Polymer, Protein Characterization□
AF2000 MT: Mid Temp Asymmetric Flow Field-Flow Fractionation for Particle, Polymer, Protein Characteriz.□
AF2000 HT: High Temperature Asymmetric Flow Field-Flow Fractionation for Polyolefin Characterization□
TF2000: Thermal Field-Flow Fractionation for advanced polymer characterization in organic solvents□
S101: Sedimentation Field-Flow Fractionation for high resolution particle size separation□
SF1000: SPLITT- Field-Flow Fractionation for continuously preparative scale particle separationHPLC & Syringe Pumps – Laser Light Scattering/UV/RI/FL Detectors – Degassers - Autosampler
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PN1130 Pump Series: Double piston pumps for HPLC, SEC, GPC and Micro Reaction technology□
PN1600 Pump Series: Double piston syringe pumps for HPLC, SEC, GPC and Micro Reaction technology□
PN3000 MALS Series: Multi Angle Laser Light Scattering (molecular weight, size determination)□
PN3000 DLS Series: Dynamic Laser Light Scattering (molecular/particle size determination)□
PN3000 XPT Series: Optical Particle Detection for Microparticles – Size, number and shape determination□
PN3100 RI Series: Refractive Index detector with high sensitivity and base line stability□
PN3200 UV Series: 1-4-Channel variable wavelength detector for FFF, SEC and HPLC□
PN3400 FL Series: Fluorescence detector with proven sensitivity and performance□
PN5100 Manual Injectors: Manual Rheodyne sample injectors for FFF, SEC and HPLC□
PN5300 Automatic Injectors: Automatic sample injectors for FFF, SEC and HPLC□
PN7500 Degasser: On-line Degassers with 2 to 4 Channels for aqueous and organic solvents□
PN8000 Fraction CollectorsConsumables for HPLC, GPC, CE, GC and Liquid Handling
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Upchurch Scientific: Chromatography consumables, tubings and fittings for fluid transfer applications□
Hamilton: Polymeric HPLC columns, GC columns, GC liner, micro syringes, pipettes and dispenser□
Rheodyne OEM: Sample injection valves, switching valves, fittings, degasser and debubbler□
Millipore: Analytical Sample Preparation and solvent filtration□
MicroSolv: CE Columns, capillaries, buffers, consumables, solutions for CEC, silica HPLC columns□
Ismatec: High quality peristaltic, tubing, gear pumps and supplies□
Spectral Lamps: Deuterium lamps, tungsten lamps, Xenon and hollow cathode lamps for LC and AAS□
Syringe Filters & Vials: Chromatography syringe filter, vials, GPC columns for various applications□
Fused Silica Tubings: Broadest range of fused silica capillaries with various dimensions (ID/OD)□
Nanoparticles: Catalog with more than 300 particle sizes, surfaces and materials□
Polymer Standards: Catalog with all kind of polymers and the broadest range available on the market□
GPC Columns: High quality GPC/SEC columns; high throughput, analytical and prep for various applicationsAnalytical Services FFF and Light Scattering
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Application Development with Field-Flow Fractionation – Static / Dynamic Laser Light Scattering□
Characterization of bio macromolecules, polymers and nanoparticles□
Trainings, Work Shops and Seminars in the European and American Application Centers - EAC / AAC
Title: First Name: Last Name:
Company: Department: Street/P.O. Box: _________________________________________ ZIP/City/Country: Telephone: Fax: _________________________________________ Email: Web:
