OIE Reference Laboratory Reports Activities

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OIE Reference Laboratory Reports Activities

Activities in 2014

This report has been submitted : 2015-02-12 10:27:41

Name of disease (or topic) for which you are a

designated OIE Reference Laboratory: Viral encephalopathy and retinopathy

Address of laboratory: Istituto Zooproﬁlattico Sperimentale delle Venezie

Viale Dell'Università, 10 35020 Legnaro (Padova) Italy ITALY

Tel.: +39-049 808 4371

Fax: +39-049 808 4360

E-mail address: [email protected]

Website: www.izsvenezie.it

Name (including Title) of Head of Laboratory

(Responsible Oﬃcial): Dr Giovanni Cattoli, DVM, PhD

Name (including Title and Position) of OIE

Reference Expert: Dr Giovanni Cattoli, DVM, PhD

Which of the following deﬁnes your laboratory? Check all that apply:

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ToR 1: To use, promote and disseminate diagnostic methods validated according to

OIE Standards

1. Did your laboratory perform diagnostic tests for the specified disease/topic for purposes such as disease diagnosis, screening of animals for export, surveillance, etc.? (Not for quality control, proficiency testing or staff training)

Yes

Diagnostic Test Indicated in OIEManual

(Yes/No) Total number of test performed last year Indirect diagnostic tests Nationally Internationally

Seroneutralization no 128 68

Direct diagnostic tests Nationally Internationally

Cell Culture (SSN-1) yes 212 11

Real-time PCR(rPCR) yes 829 727

Immunohistochemistry (IHC) yes 23 7 Molecular characterization (RT-PCR and

sequencing analysis) yes 19 15

ToR 2: To develop reference material in accordance with OIE requirements, and

implement and promote the application of OIE Standards.

To store and distribute to national laboratories biological reference products and

any other reagents used in the diagnosis and control of the designated pathogens

or disease.

2. Did your laboratory produce or supply imported standard reference reagents oﬃcially recognised by the OIE? No

3. Did your laboratory supply standard reference reagents (non OIE-approved) and/or other diagnostic reagents to OIE Member Countries?

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Type of reagent available Related diagnostic test Produced/ provide Amount supplied nationally (ml, mg) Amount supplied internationally (ml, mg) No. of recipient OIE Member Countries Region of recipients Rabbit polyclonal serum anti betanodavirus (genotype RGNNV) IHC IF SN ELISA Stored 1ml 2ml 2 Africa America s Asia and Paciﬁc Europe Middle East Rabbit polyclonal serum anti betanodavirus (genotype SJNNV) IHC IF SN ELISA Stored 1ml 0 0 Africa America s Asia and Paciﬁc Europe Middle East Negative sea

bass serum SN ELISA Stored 0 3ml 1

Africa America s Asia and Pacific Europe Middle East Lyophilized inactivated betanodavirus (reference strain) RT-PCR rRT-PCR Stored 0 1ml 1 Africa America s Asia and Pacific Europe Middle East Lyophilized live betanodavirus (reference strain) all diagnostic tests Stored 0 2ml 2 Africa America s Asia and Pacific Europe Middle East

SSN-1 cell line Cell culture_isolation _{and Stored}Produced 0 cm2; 1 ﬂask of5 ﬂasks of 25

75 cm2 (40 ml) 3 Africa America s Asia and Paciﬁc Europe

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E-11 cell line Cell culture_isolation _{and Stored}Produced 0 2 ﬂasks of 25_{cm2 (10 ml)} 1 Africa America s Asia and Paciﬁc Europe Middle East

4. Did your laboratory produce vaccines? No

5. Did your laboratory supply vaccines to OIE Member Countries? No

ToR 3: To develop, standardise and validate, according to OIE Standards, new

procedures for diagnosis and control of the designated pathogens or diseases

6. Did your laboratory develop new diagnostic methods validated according to OIE Standards for the designated pathogen or disease?

No

7. Did your laboratory develop new vaccines according to OIE Standards for the designated pathogen or disease? Yes

Name of the new test or diagnostic method or

vaccine developed Description and References (Publication, website, etc.)

Real Time RT-PCR

Baud, M., Cabon, J., Salomoni, A., Toﬀan, A., Panzarin, V., & Bigarre, L. (2014). First generic one step real-time Taqman RT-PCR targeting the RNA1 of betanodaviruses. Journal of virological methods, doi:S0166-0934(14)00371-1

[pii]

ToR 4: To provide diagnostic testing facilities, and, where appropriate, scientiﬁc

and technical advice on disease control measures to OIE Member Countries

8. Did your laboratory carry out diagnostic testing for other OIE Member Countries? Yes

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Name of OIE Member

Country seeking assistance Date (month)

No. samples received for provision of diagnostic support

No. samples received for provision of

conﬁrmatory diagnoses SPAIN from Jannuary to December₂₀₁₄ 500 3

PORTUGAL March 35

CYPRUS June 10

CYPRUS October 3

TUNISIA from April to August 19 GREECE from June to November 31

SAUDI ARABIA February 23 2

SAUDI ARABIA from March to October 66

UNITED KINGDOM February 4

UNITED KINGDOM March 8

BAHRAIN from April to August 18 2

TURKEY October 4

9. Did your laboratory provide expert advice in technical consultancies on the request of an OIE Member Country? Yes

Name of the OIE Member Country

receiving a technical consultancy Purpose How the advice wasprovided TUNISIA Molecular epidemiology of VER in Tunisia Remote SAUDI ARABIA Diagnosis and control of VER Remote CYPRUS Diagnosis and control of VER and other ﬁsh_diseases Remote

ToR 5: To carry out and/or coordinate scientiﬁc and technical studies in

collaboration with other laboratories, centres or organisations

10. Did your laboratory participate in international scientiﬁc studies in collaboration with OIE Member Countries other than the own?

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Title of the

study Duration Purpose of thestudy Partners (Institutions)

TARGETFISH 5 years Develop implementation of vaccines for European ﬁsh farming

1. Netherlands Wageningen University 2. Danmarks Tekniske Universitet (DTU), Denmark 3. The University

Court of the University of Aberdeen (UA), United Kingdom 4. The Scottish Ministers Acting Through Marine

Scotland (MS), United Kingdom 5. Friedrich Löﬄer Institut - Bundesforschungsinstitut Für Tiergesundheit (FLI), Germany 6. Instituto Nacional De Investigacion Y

Tecnologia Agraria Y Alimentaria (INIA), Spain 7. Universitat Autonoma de Barcelona (UAB), Spain 8. Universita Degli Studi Della Tuscia (UT), Italy 9. Institut National De La Recherche Agronomique (INRA), France 10. Veterinaerhogskole (NV), Norway 11. The University

Of Stirling (US), United Kingdom 13. Københavns Universitet (KU), Denmark 14. Výzkumný ústav veterinárního lékařství (VRI), Czech Republic 15. The

Hebrew University of Jerusalem (HUJ), Israel 16. University of Murcia (UM), Spain 17. Tethys Aquaculture

Limited (TET), Greece 18. PatoGen Analyses AS (PG), Norway 19. Fishlab (FL), Denmark 20. Naxo OÜ (NX), Estonia 21. Ridgeway Biologicals (RBL), United Kingdom

22. Rossi A/S (ROS), Denmark 23. Ingeniatrics Tecnologias S.L. (ING), Spain 24. BigDNA (BD), United Kingdom 25. Biotechnology GmbH (W42), Germany 26.

AQUARK, Greece 27. CentroVet (CV), Chile 28. Dansk akvakultur forening (DA), Denmark 29. BioMar A/S (BM),

Denmark 30. Bionaturis (BN), Spain

INNOV-H20 3 years

Network for Technological

Innovation in Aquaculture

1. Università degli Studi di Trieste 2. Università degli Studi di Padova 3. Università degli Studi di Udine 4. Univerzav Novi Gorici 5. Shoreline Soc.Coop. 6. Vodomec

d.o.o.

MOLTRAQ 3 years

Molecular tracing of viral pathogens

in aquaculture

1. National Veterinary Institute 2. Tecnical University of Denmark (DTU-VET), Denmark 3. Agence Nationale de Sécurité Sanitaire (ANSES), France 4. Institut Francais de Recherche pour Léxploitation de la Mer (Ifremer), France 5. Institut de Recherche pour le Développement (IRD),

France 6. Friedrich-Loeﬄer Institut (FLI), Germany 7. Norwegian Computing Centre (NR), Norway

ToR 6: To collect, process, analyse, publish and disseminate epizootiological data

relevant to the designated pathogens or diseases

11. Did your Laboratory collect epizootiological data relevant to international disease control? Yes

12. Did your laboratory disseminate epizootiological data that had been processed and analysed? Yes

13. What method of dissemination of information is most often used by your laboratory? (Indicate in the appropriate box the number by category)

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a) Articles published in peer-reviewed journals: 3

Baud, M., Cabon, J., Salomoni, A., Toﬀan, A., Panzarin, V., & Bigarre, L. (2014). First generic one step real-time Taqman RT-PCR targeting the RNA1 of betanodaviruses. Journal of virological methods,

doi:S0166-0934(14)00371-1 [pii]

Haddad-Boubaker, S., Boughdir, W., Sghaier, S., Ben Suissi, J., Megdich, A., Dhaouadi, R., Amara, A., Panzarin, V., Fakhfakh, E. (2014). Outbreak of viral nervous necrosis in endangered ﬁsh species epinephelus costae and E. marginatus in northern tunisian coasts. Fish Pathology, 49(2), 53-56. 492

Panzarin, V., Cappellozza, E., Mancin, M., Milani, A., Toﬀan, A., Terregino, C., Cattoli, G. (2014). In vitro study of the replication capacity of the RGNNV and the SJNNV betanodavirus genotypes and their natural reassortants in response to temperature. Veterinary Research, 45(1), 56-9716-45-56. doi:10.1186/1297-9716-45-56 [doi]451 b) International conferences: 3

Baud, M., Cabon, I., Salomini, A., Panzarin, V., Toﬀan, A. & Bigarré, L. (2014). Validation of a one-step generic taqman rRT-PCR targeting betanodaviruses. 9th International Symposium on Viruses of Lower Vertebrates, 1-4 October 2014, Malaga (Spain)

Buonocore, N., Nunez, A., Stocchi, V., Picchietti, S., Randelli, E., Papeschi, C., Toﬀan, A., Pascoli, F., Lorenzen, N., Thompson, K. & Scapigliati, G. (2014). The encefalopathy and retinopathy virus of European sea bass

dicentrarchus labrax: strategies for its detection and juveniles immunization. 9th International Symposium on Viruses of Lower Vertebrates, 1-4 October 2014, Malaga (Spain)

Wiegertjes, G., Lorenzen, N., Secombes, C., Collet, B., Fischer, U., Tafalla, C., Parra, D., Scapigliati, G., Boundinot, p., Evensen, Ø., Adams, A., Toffan, A., Buchmann, K., Vesely, T., David, L., Mulero, V., Smith, P., Aspehaug, V., Engell-Sørensen, K., Sober, J., Wallis, T., Rød, T., Flores, M., March, J., Stratmann, A., Christofilogiannis, P., Tobar, J., Hennriksen, N., Sigholt, T., De las Heras, A. (2014). Targeted disease prophylaxis in European fish farming (TARGETFISH). 18th Annual Workshop of the National Reference Laboratories for Fish Diseases 3-4 June 2014 Copenhagen (Denmark)

c) National conferences: 3

Ceolin, C., Bille, L., Toson, M., Toﬀan, A., QUartesan, R., Pascoli, F., Casarotto, C. & Dalla Pozza, M. (2014). Encefaloretinopatia virale in Italia dal 2008 al 2013. Atti del XX Convegno Nazionale S.I.P.I. Società Italiana di Patologia Ittica, 18-19 settembre 2014, Certosa di Calci - Pisa (Italy)

Toﬀan, A., Buratin, A., Ferro, D., Abbadi, M., Panzarin, V., Mancin, M. & Cattoli, G. (2014). Caratterizzazione sierologica di diversi genotipi di betanodavirus. Atti del XX Convegno Nazionale S.I.P.I. Società Italiana di Patologia Ittica, 18-19 settembre 2014, Certosa di Calci - Pisa (Italy)

Toﬀan, A., Fusco, G., Patarnello, P., Panzarin, V., Pascoli, F., Ceolin, C., Lucifora, G., Amoroso, M.G., Viscardi, M., Di Nocera, F., Purpari, G., La Cavera, E., Guercio, a. & Marino, F. (2014). Moria anomala di cernie selvatiche nel sud tirreno: un caso di Betanodavirosi. Atti del XX Convegno Nazionale S.I.P.I. Società Italiana di Patologia Ittica, 18-19 settembre 2014, Certosa di Calci - Pisa (Italy)

d) Other:

(Provide website address or link to appropriate information) 4

http://www.izsvenezie.it/istituto/centri-di-referenza-nazionale/malattie-dei-pesci-molluschi-e-crostacei/ http://targetﬁsh.eu/participants

http://it.innov-h2o.eu/

https://moltraq.wordpress.com/author/moltraq/

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Countries

To recommend the prescribed and alternative tests or vaccines as OIE Standards

14. Did your laboratory provide scientiﬁc and technical training to laboratory personnel from other OIE Member Countries?

Yes

a) Technical visits: 0 b) Seminars: 0

c) Hands-on training courses: 0 d) Internships (>1 month): 0

Type of technical training

provided (a, b, c or d) Country of origin of the expert(s)provided with training No. participants from thecorresponding country

ToR 8: To maintain a system of quality assurance, biosafety and biosecurity

relevant for the pathogen and the disease concerned

15. Does your laboratory have a Quality Management System certiﬁed according to an International Standard? Yes

Quality management system adopted Certiﬁcate scan (PDF, JPG, PNG format) ISO 17025 certiﬁcato-ISO-17025.pdf 16. Is your laboratory accredited by an international accreditation body?

Yes

Test for which your laboratory is accredited Accreditation body

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---17. Does your laboratory maintain a “biorisk management system” for the pathogen and the disease concerned? Yes

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ToR 9: To organise and participate in scientiﬁc meetings on behalf of the OIE

18. Did your laboratory organise scientiﬁc meetings on behalf of the OIE? No

19. Did your laboratory participate in scientiﬁc meetings on behalf of the OIE? No

ToR 10: To establish and maintain a network with other OIE Reference Laboratories

designated for the same pathogen or disease and organise regular inter-laboratory

proﬁciency testing to ensure comparability of results

20. Did your laboratory exchange information with other OIE Reference Laboratories designated for the same pathogen or disease?

No

21. Was your laboratory involved in maintaining a network with OIE Reference Laboratories designated for the same pathogen or disease by organising or participating in proﬁciency tests?

No

22. Did your laboratory collaborate with other OIE Reference Laboratories for the same disease on scientiﬁc research projects for the diagnosis or control of the pathogen of interest?

No

ToR 11: To organise inter-laboratory proﬁciency testing with laboratories other

than OIE Reference Laboratories for the same pathogens and diseases to ensure

equivalence of results

23. Did your laboratory organise or participate in inter-laboratory proﬁciency tests with laboratories other than OIE Reference Laboratories for the same disease?

No

Note: See Interlaboratory test comparisons in: Laboratory Proﬁciency Testing at:

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ToR 12: To place expert consultants at the disposal of the OIE

24. Did your laboratory place expert consultants at the disposal of the OIE? No