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A localized tolerance in the substrate specificity of the fluorinase enzyme enables "last step" 18F fluorination of a RGD peptide under ambient aqueous conditions

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Figure

Figure 2. HPLC monitoring 15 to 16 by the fluorinase. Conditions: KF, L-SeMet, fluorinase, phosphate buffer, pH 7.8, 37°C
Figure 3.  HPLC (radio and UV) traces of enzymatic transformation of 15 to [18F]-16 (30 min, rt)
Figure 4  Figure 4: Summed transaxial (Trans, L) and saggital (Sag, R) PET images of a rat, 5-60min following administration of 5MBq of [18F]FDA-TEG RGD, co-registered with corresponding CT images (grey scale)

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