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Synthesis, Biophysical Characterization and in Vitro Transfection Activity of Novel Bivalent Amine Cationic Lipids in the Absence of Dioleoylphosphatidylethanolamine (DOPE)

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Figure 1. (a) Fluorescence of EGFP in B16F0 cells transfected with lipoplexes of MDVAD, ODVAD and DOTAP as a positive control at +/− charge ratio of 2:1, in the ab-sence of helper lipids
Figure 2. (a) Gel retardation assay of lipoplexes in the absence of helper lipids in 40 mM tris buffer pH 7.2
Table 1. Particle size and zeta potential measurements of cationic lipid dispersions and lipoplexes, at various charge ratios, in 40 mM tris solution pH 7.2
Table 2. Phase transition parameters of bivalent amine cationic lipids as determined by fluo-rescence anisotropy

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