Todd C. Lorenz, Ph.D.
Phone 909.593.3511 1950 3rd Street, FH 109D [email protected] La Verne, CA 91750
EDUCATION
Ph.D., Biological Chemistry, University of California, Los Angeles (2007) M.S., Chemistry, DePaul University, Chicago, Illinois (1999)
B.S., Chemistry, DePaul University, Chicago, Illinois (1998)
TEACHING EXPERIENCE
RESPONSIBILITIES
Develop and teach discovery-based courses
Present instructional materials
Generate and grade quizzes, exams, homework assignments, and laboratory practicum
Oversee performance of student experiments
Develop assessment strategies
Supervise teaching assistants
Develop and edit MIMG Microbe Journal
Develop and assess new microbiology course for nursing students UNIVERSITY OF LA VERNE APPOINTMENTS
Assistant Professor 2012 – Present Department of Biology
UNIVERSITY OF LA VERNE COURSES
Bio101 – Life Science (Non-majors Biology Course with Laboratory) UNIVERSITY OF CALIFORNIA, LOS ANGELES APPOINTMENTS Lecturer 2008 – 2012
Department of Microbiology, Immunology, and Molecular Genetics UCLA COURSES
MIMG 109AL – Research Immersion Laboratory in Microbiology (Discovery-based course in microbial ecology)
MIMG 103AL – Research Immersion Laboratory in Virology (Discovery-based course in phage biology)
MIMG 100L – Microbiology Laboratory for Professional Schools (Non-majors course for development of experimental techniques of microbiology)
MIMG 10 – Medical Microbiology for Nursing Students (Introduction to biology of microbial pathogens)
LS 1 – Evolution, Ecology & Biodiversity UCLA Life Sciences Core Curriculum COMMUNITY COLLEGE APPOINTMENTS
Los Angeles Southwest College Lecturer February 2009- June 2009
Department of Natural Science and Physical Education
PREVIOUS LOS ANGELES SOUTHWEST COLLEGE COURSES
CHEM 051 – Fundamentals of Chemistry (Non-majors Chemistry Course with Laboratory)
CERRITOS COLLEGE Lecturer 2008 – 2009 Biology Department
PREVIOUS CERRITOS COLLEGE COURSES
A&P 151 – Intro to Human Physiology (Majors Human Anatomy & Physiology Course with Laboratory)
A&P 120 – Intro Human Anatomy & Physiology (Non-majors Human Anatomy & Physiology Course with Laboratory)
BIO 120 – Introduction to Biological Sciences (Non-majors Biology Course with Laboratory)
ADULT LEARNING APPOINTMENTS
UNIVERSITY OF CALIFORNIA, LOS ANGELES EXTENSION Lecturer 2009
Department Science and Math
PREVIOUS UCLA EXTENSION COURSES
Life Sciences XL 4 – Genetics UCLA Life Sciences Core Curriculum
RESEARCH INTERESTS
Metagenomic analysis of microbial communities
Microbial energy production
Bacteriophage discovery
Bacteriophage therapies
Protein trafficking and vesicle formation
PUBLICATIONS
Sabine Gorynia, Todd C. Lorenz, Giancarlo Costaguta, Lydia Daboussi, Dulio Cascio, and Gregory S. Payne (2012) Yeast Irc6p is a novel type of conserved clathrin accessory factor related to G proteins Molecular Biology of the Cell E12-07-0507; First Published on September 19, 2012; doi:10.1091/mbc.E12-07-0507 Todd C. Lorenz (2012) Polymerase Chain Reaction: Basic Protocol Plus Troubleshooting and Optimization Strategies: J. Vis. Exp. (63), e3998 10.3791/3998
http://www.jove.com/video/3998/
Anand VC, Daboussi L, Lorenz Todd C, and Payne GS (2009) Genome-wide analysis of AP-3 dependent protein transport in yeast. Molecular Biology of the Cell 20(5): 1592-604.
Lorenz Todd C, Anand VC, and Payne GS (2008) High-throughput protein extraction and immunoblotting analysis in Saccharomyces cerevisiae. Methods in Molecular Biology 457, ISBN: 978-1-58829-925-3.
Zegzouti H, Li W, Lorenz TC, Xie M, Payne CT, Smith K, Glenny S, Payne GS, and Christensen SK (2006) Structural and functional insights into the regulation of Arabidopsis AGC VIIIa kinases. Journal of Biological Chemistry 281: 35520-30. Parsons AB, Lopez A, Givoni IE, Williams DE, Gray CA, Porter J, Chua G, Sopko R, Brost RL, Ho CH, Wang J, Ketela T, Brenner C, Brill JA, Fernandez GE, Lorenz TC, Payne GS, Ishihara S, Ohya Y, Andrews B, Hughes TR, Frey BJ, Graham TR, Andersen RJ, Boone C (2006) Exploring the mode-of-action of bioactive compounds by chemical-genetic profiling in yeast. Cell 126: 611-25.
RESEARCH EXPERIENCE
2008-2009
Staff Research Associate. Report to Gregory S. Payne. David Geffen School of Medicine at UCLA, Department of Biological Chemistry.
2002-2008
Graduate Student Research. Dissertation research in laboratory of Gregory S. Payne: Investigated clathrin-mediated protein trafficking using the model organism Saccharomyces cerevisiae. David Geffen School of Medicine at UCLA, Department of Biological Chemistry.
1999-2002
Staff Research Associate. Reported to Gregory S. Payne. Examined the role of auxilin in uncoating of clathrin-coated vesicles. David Geffen School of Medicine at
1996-1998
Undergraduate Research Assistant. Independent research project in laboratory of Kathleen Helm-Bychowski. Conducted phylogenetic analysis of migratory birds using cytochrome genes. DePaul University, Chemistry Department.
RESEARCH PRESENTATIONS
Lorenz TC, Kim M, Xinhuang, Di Pietro S, Wohlschlegel J, and Payne GS (2007) Accessory proteins involved in clathrin-mediated transport between the trans Golgi network and endosomes. UCLA Dept. Biological Chemistry Annual Retreat, Lake Arrowhead, CA.
Lorenz TC and Payne GS (2005) Yeast as a model to study prostate specific membrane antigen UCLA Dept. Biological Chemistry Annual Retreat, Skirball Cultural Center, Los Angeles, CA.
Lorenz TC and Payne GS (2003) Systematic genetic and phenotypic analysis of protein traffic in yeast: Accessory proteins involved in clathrin-mediated transport between the trans Golgi network and endosomes. UCLA Dept. Biological Chemistry Annual Retreat, Lake Arrowhead, CA.
Pishvaee B, Lorenz TC, and Payne GS (2001) Analysis of yeast auxilin and clathrin interaction. Yeast Cell Biology Meeting, Cold Spring Harbor Laboratory, Cold Spring Harbor, NY.
SPECIALIZED SKILLS
Microbiology
Microbial ecology using cultivation dependent and cultivation independent methods to profile soil bacterial.
Isolation and identification of novel mycobacterium phage form soil samples. Isolation and identification of novel propionibacterium phage form integumentary samples.
Bioinformatics/Proteomics:
Excel-based analysis of large data sets from different experimental conditions used to create ven diagrams. Generated mini-arrays of yeast strains with deletions of protein-trafficking genes, which were derived from analysis of data produced in large synthetic genetic arrays (SGAs) from the yeast knock-out collection in collaboration with the C. Boone laboratory. Developed a rapid immunoblotting-based method to assess defects in trafficking pathways.
Planctomyces limnophilius DSM 3376 genome annotation project in collaboration with the DOE Joint Genome Institute.
Protein purification and characterization: Immunoblotting & Immunoprecipitation
Expression and affinity-chromatography purification of recombinant proteins (e.g., GST-, Histidine-, and TAP-tagged)
Yeast two-hybrid analysis
FPLC S-1000 – purification of clathrin coated vesicles Antibody production:
Preparation of immunogen and affinity purification of antisera, resulting in antibodies capable of recognizing the endogenous antigens under both native and denaturing conditions
Molecular Genetics: PCR mutagenesis
Cloning of DNA constructs (e.g., vectors for yeast expression, GFP and GST fusion, yeast two-hybrid,)
Yeast Genetics:
Tetrad dissection
Targeted gene disruption
Recombinant genomic insertion of GFP, MYC and HA epitope tags Cell biology:
Fluorescence microscopy Differential centrifugation
PROFESSIONAL SERVICE & ORGANIZATIONAL MEMBERSHIPS
The American Society for Cell Biology (2001-2002, 2007-2009) The American Society for Microbiology (2007-2009, 2012- Present) BC Retreat Panel Discussion on Alternative Career Development (2007) Biological Chemistry Graduate Student Council Representative (2002-2003) UCLA Yeast Super Group (2001)
