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Regulation and function of the tissue specific transcription factor HNF1 alpha (LFB1) during Xenopus development

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Figure

Fig. 2. The interaction of HNF1a with the HPIelement.The tetramerconsisting of HNF1a and DCoH dimer is shown as bound to the regula-tory erementHP 1 upstreamof the TATA-box and the transcriprion starrsite (arrow)
Fig. 4. Interactionof XDCoHandHNF1a.UsingthelabeledHPIoligonucleotide, gel retardation assays were made with 0.2 or 1 J-liHNF1a (XHNFlaa)and 5 J-llXenopusDCoH translatedin a reticulocytelysate.The addition of the monoclonalantibody XAOI specificfor HNF1a(B
Fig. 5. Immunostaining ofpicturesto locate HNF1a.anti-mousemagnificationliverand gutsectionsof adultXenopus Cryostatsectionsof the liver (a) and the gut (b) wereimmunostainedwith the HNF1exspe-cificmonoclonalantibodyXAD5(Bartkowskiet al., 1993) as first an
Fig. 6. Developmentalappearanceof HNF1cr: mRNA, HNF1cr: protein,DCoH proteinandalbuminmRNA.The amountof HNFlamRNAandHNFla protein at various stages of development are given as determined in Bartkowski et al
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