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Analysis of parent specific gene expression in early mouse embryos and embryonic stem cells using high resolution two dimensional electrophoresis of proteins

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Academic year: 2020

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Figure

Fig. 1. Proteindetectionat the blastocyststage.(A)Two dimensionalgel imageof proteinssynthesizedby 50 normal blasrocysts.The proteinswere labeledwithL-[3sS1methionineand separatedon gels in the 20-100kOa range.The gel was processedfor fIuorographyand expos
Fig. 2. Reproducibilityof detectionattheblastocyststage.(A)Histogramshowingthe reproducibilityof protein detection In the five gelsof normalblastocysts.Of 837 spors.93%had a percentageerrorof <50,86% had a percentageerror of <40, 70% had a percentageerror of <30.46%had a percentageerror<20 and12% had a percentageerror<10Only837 out of the 1125 presentin rhe standard were analyzed.This isbecausea minimumof twoIntensityvaluesis requiredfor rhe calculationof standard deviation (B) Histogramshowrngthe reproducibilityof pro-teindetectionin thefivegelsof parthenogeneticblastocystsOf 942spots.92%had a percentageerror of <50, 85%had a percentageerrorof <40,73% had a percentageerror of <30, 47%had a percentageerror<20 and12% had a percentageerror < 10 Only 942 out of the1125 pre-sent in the standardwere analyzed
Fig. 3. Two dimensionalgel imageof proteinssynthesizedby 50normalblastocysts.The proteins were labeled with L-p5S1methionineand separated on gels in the 5-25 kOa range
Fig. 4. Proteindetectionin embryonicstemcells. (AI Two dimensionalgel image of proteinssvnthesizedin embryonic stem cells
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