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Ovine recombinant PrP as an inhibitor of ruminant prion propagation in vitro

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Figure

FIGURE 1. Ovine PrPs rVRQ, rARQ and rARR purified by IMAC. A) Purification of rVRQ. Lane 1:rVRQ from urea solubilised washed inclusion body
FIGURE 2. Inhibition of ovine prion replication with distinct rPrPs. A single classical scrapie sample(PG1361/05) was amplified in triplicates by PMCA for one round using a VRQ PrPC substrate.PMCA reaction products amplified in the presence or absence of rVR
FIGURE 3. Both native and denatured rVRQinhibit prion replication. PMCA amplification of ascrapie isolate (PG1361/05) was performed intriplicate in the presence of 1200 nM of rVRQwith or without denaturation or in the absence ofany rPrP (as indicated)
FIGURE 4. rVRQ binds to PrPSc. Beads werecoated with rVRQ (rVRQ-beads capture) or hadno rPrP present (bead capture) and were thenincubated with brain homogenate from a scra-pie-affectedsheep(PG1361/05).Followingextensive washing, the beads were used toseed
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