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Differentiation of neuronal cells using a murine embryonic stem cell based method

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Figure

Figure 1. Targeting vector with Necdin promoter-selection- reporter (pNec-SR). Plasmid pUC19 was modified with restric- tion sites by sequential ligation of oligolinkers and the follow- ing gene fragments were sequentially cloned: a neomycin gene (Neo) und
Figure 4. Puromycin selection (day 12). (A) Beta-galactosidase activity measured in cells treated without (− selection) and with (+ selection) puromycin

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