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Cloning of a muscle specific calpain from the American lobster Homarus americanus: expression associated with muscle atrophy and restoration during moulting

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Academic year: 2020

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Figure

Fig. 2. Comparison of Ha-CalpM amino acid (aa) sequence with calpains from Drosophilanumbers are Z46891 for  and human
Fig. 3. Phylogenetic relationship of Ha-Dm-CalpB-IIHa-CalpM-IIhuman p94-IInCL-4-IIhuman m-IICapn5 (mouse)-IItra-3-IIShistosoma mansoniDm-CalpA-II26.72520151050 calpains and human m-calpain are given in the legend of Fig
Fig. 6. SDS-PAGE and western blot analysis of Ha-CalpM in lobstertissues. Supernatant fractions (32 µg protein) from various tissueswere electrophoresed on 6% SDS-polyacrylamide gels and eitherstained with Coomassie Blue (C) or transferred onto PVDFmembran
Fig. 7. Immunocytochemical localization of Ha-CalpM in claw muscles from adult lobster
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