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Mammalian Clusterin associated protein 1 is an evolutionarily conserved protein required for ciliogenesis

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Figure 1 Clusterin associated protein 1 (Cluap1) knockout mice are embryonic lethal. (A) Schematic of the wild-type Cluap1 allele (Cluap1WT)and the Cluap1 knockout allele (Cluap1KO)
Figure 3 Cluap1 localizes to primary cilia20 in vitro. Antibody against acetylated α-tubulin (red) and Cluap1 (green) label primary cilia (arrows) in(A-C) NIH3T3 cells (scale bars are 14 μm)
Figure 4 Cluap1KO embryos fail to form primary cilia. (A,C,E) Cluap1WT E9.5 embryos were immunolabeled for the cilia marker acetylatedα-tubulin (red) and Cluap1 (green) in the lateral plate mesenchyme of Cluap1WT embryos
Figure 5 Cluap1KO embryos have defects in floorplate induction. (A,C,E) Cluap1WT E9.5 embryos stained for Arl13b (green) show cilia in theneural tube and surrounding tissue
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