16S Rrna
Analysis of 525 Samples To Determine the Usefulness of PCR Amplification and Sequencing of the 16S rRNA Gene for Diagnosis of Bone and Joint Infections
... femur biopsy retrieved only S. aureus. Two 16S rRNA gene PCR assays performed on the same sample detected C. perfringens, and a specific PCR confirmed these results. Spe- cific S. aureus PCR detected the ...
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Detection of 16S rRNA Methylase Genes in Gram Negative Bacilli Isolated from Hospitals in Changchun, China
... main 16S rRNA methylase genes in the Changchun area, which is consistent with relevant domestic studies [16, ...detected 16S rRNA methylase genes in European countries such as Belgium [20] and ...
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Comparison of PCR Electrospray Ionization Mass Spectrometry with 16S rRNA PCR and Amplicon Sequencing for Detection of Bacteria in Excised Heart Valves
... for 16S rRNA PCR was thawed under a class 2 biosafety cabinet for molecular testing, and 300 l ( ⫾ 35 mg of tissue) was chemically and mechanically lysed on the Iridica bead-beater and an extraction ...
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Three Cases of Anaerobiospirillum succiniciproducens Bacteremia Confirmed by 16S rRNA Gene Sequencing
... Antimicrobial susceptibility testing by various investigators (5, 15) has shown that clinical isolates of A. succiniciproducens are susceptible to carbenicillin, chloramphenicol, and cephalo- thin and are resistant to ...
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Sequence Diversity of Neisseria meningitidis 16S rRNA Genes and Use of 16S rRNA Gene Sequencing as a Molecular Subtyping Tool
... of 16S rRNA genes among Neisseria meningitidis strains (Men) and evaluated the use of this approach as a molecular subtyping ...the 16S rRNA gene from 264 Men strains of serogroups A, B, C, ...
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Temperature Gradient Gel Electrophoresis of Fecal 16S rRNA Reveals Active Escherichia coli in the Microbiota of Patients with Ulcerative Colitis
... TTGE separates bacterial DNA fragments with similar sizes but different levels of thermal stability (22, 31). Sequences differing by a single base can be separated by this method. Applied to complex microbial ...
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Prediction and Modeling of the Structure of 16S rRNA
... between 16S rRNA nt 121 and 124 in the major groove of the helical ...the 16S rRNA at 595(596:644) has been shown to occur in the major groove (Kalurachchi and Nikonowicz, 1998) with the ...
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Identification of YbeY Protein Interactions Involved in 16S rRNA Maturation and Stress Regulation in Escherichia coli
... as 16S rRNA 3= end maturation, 70S ribosome quality control, and regula- tion of mRNAs and small noncoding RNAs, thereby affecting cellular viability, stress tolerance, and pathogenic and symbiotic behavior ...
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Molecular detection by analysis of the 16S rRNA gene of fecal coliform bacteria from the two Korean Apodemus species (Apodemus agrarius and A. peninsulae).
... the 16S rRNA gene molecular diagnostic ...bacterial 16S rRNA gene markers, we detected four different bacterial species (Bacillus amyloliquefaciens, Escherichia coli, Staphylococcus xylosus, ...
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SSCP Analysis of V3 Region of 16S rRNA for the Characterization of Rumen Microbiome of Goat
... bacterial 16S rRNA gene and V3 region of 16S rRNA are given in table ...of 16S rRNA was performed by thermal cycler (Mastercycler EP, Eppendorf) using the program: initial ...
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Complete genome sequence of the motile actinomycete Actinoplanes missouriensis 431T (= NBRC 102363T)
... tween the genome data and the reported 16S rRNA gene sequence are likely due to sequencing errors in the previously reported sequence data.. The 16S rRNA sequence of strain 431 T did[r] ...
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Association between calcifying nanoparticles and placental calcification
... Methods: Calcified placental tissues and distal tissue samples were collected from 36 confirmed placental calcification cases. In addition, 20 normal placental tissue samples were obtained as a control group. All the ...
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Genomics of the Uncultivated, Periodontitis-Associated Bacterium Tannerella sp. BU045 (Oral Taxon 808)
... containing rRNA genes. The rRNA-containing contigs were used for a BLAST (38) search of the NCBI refseq_genomic database, restricted to bacterial ...the 16S rRNA gene matched with greater than ...
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Catheter associated bacteremia by Mycobacterium senegalense in Korea
... 16S rRNA gene sequence (1,393 bp) of the strain SMC- 7485 showed 100% similarities with those of Mycobacte- rium senegalense ATCC 35796 [GenBank: AF480596] and Mycobacterium farcinogenes ATCC 35753 ...
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Genomic analysis of a novel strain of Bacillus nealsonii, isolated from Surti buffalo rumen
... Aim: Whole genome sequencing and functional an- notation of Bacillus nealsonii strain AAU1, an amylo- lytic anaerobic spore forming isolate from ruminal contents of buffalo. Methods and Results: Morpho- logically, the ...
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Identification of Mycobacterium kansasiiby Using a DNA Probe (AccuProbe) and Molecular Techniques
... 16S rRNA gene. Sequencing of the first 600 bp of the 16S rRNA gene, including the species-specific region V2 of helix 10 as well as the variable V3 region of the helix 18, was per- ...
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Direct and Rapid Detection by PCR ofErysipelothrix sp DNAs Prepared from Bacterial Strains and Animal Tissues
... the 16S rRNA sequences of all four Erysipelothrix ...the rRNA gene cluster, including 16S rRNA, 23S rRNA, and the noncoding region downstream of 5S rRNA, were determined ...
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Genetic Diversity and Antimicrobial Susceptibility of Motile Aquatic Aeromonads - Volume 1 Number 1 (Jun. 2010) - IJCEA
... Aeromonas 16S rDNA gene sequences: A database containing 16S rRNA gene sequences of all validly published Aeromonas hydrophila was compiled from GenBank ...published 16S rDNA gene sequences ...
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Comparison of the Vitek MS and Bruker Matrix Assisted Laser Desorption Ionization–Time of Flight Mass Spectrometry Systems for Identification of Chryseobacterium Isolates from Clinical Specimens and Report of Uncommon Chryseobacterium Infections in Humans
... Study design and collection of isolates. This study was performed in a 1,000-bed university- affiliated medical center in Taiwan and was approved by the Institutional Review Board of the hospital (EMRP-106-105). The ...
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Genome sequence of the flexirubin-pigmented soil bacterium Niabella soli type strain (JS13-8T)
... Figure 1. Phylogenetic tree highlighting the position of the family aligned characters [8,9] of the 16S rRNA gene sequence under the maximum likelihood (ML) criterion [10]. Root- ing was done initially ...
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