18s-rRNA
Molecular detection of 18s rrna gene of candida albicans isolated from pregnant women with clinical diagnosis of vulvovaginitis
... is a common disease in women during their lifetime and occurs in diabetes patients, during pregnancy and oral contraceptives users. Although several conventional vulvovaginitis a challenge for patients and to find out ...
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Molecular Analysis of the 18S rRNA Gene of Cryptosporidium Parasites from Patients with or without Human Immunodeficiency Virus Infections Living in Kenya, Malawi, Brazil, the United Kingdom, and Vietnam
... complete 18S rRNA gene were used in the analysis of nucleotide ...the 18S rRNA gene for most species, with the highest variation in the ...
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High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis
... The high-resolution melting (HRM) assay is a novel and powerful molecular method applied to mutation detection, genotype analysis and species identifica- tion [23–26]. During double strand DNA (dsDNA) dissociation to ...
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Multiplex, DNase free one step reverse transcription PCR for Plasmodium 18S rRNA and spliced gametocyte specific mRNAs
... Plasmodium 18S rRNA RT-PCR directly from extracted whole blood without DNase treatment [21, ...Plasmodium 18S rRNAs are more than three orders of magnitude more abundant than the coding 18S ...
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Plasmodium 18S rRNA of intravenously administered sporozoites does not persist in peripheral blood
... Plasmodium 18S rRNA and their coding genes (rDNA) using sensitive quantitative reverse transcription PCR and quantitative PCR assays, ...high rRNA- to-rDNA ratios, this low level positivity was ...
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Development and validation of a real time PCR assay for the detection of clinical acanthamoebae
... 66 18S DNA sequences from Acanthamoeba ...reference 18S rRNA sequence from the National Center for Biotechnology Information (NCBI), Accession Number AF019056) was used for Taqman MGB primer and ...
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Evaluation of qPCR reference genes in two genotypes of Populus for use in photoperiod and low temperature studies
... of 18S rRNA by geNorm PLUS and BestKeeper also showed ...differences. 18S rRNA ranked as the least or second least stable gene when analyzed by geNorm PLUS while BestKeeper assigned 18S ...
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Ribosomal and non ribosomal PCR targets for the detection of low density and mixed malaria infections
... LM, 18S rRNA gene PCR-based assays (nested-PCR and/or R-qPCR) identified a similar proportion of malaria infec- tions (35%, n = 39) ...both 18S rRNA pro- ...
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Plasmodium vivax molecular diagnostics in community surveys: pitfalls and solutions
... To improve the specificity of LAMP, several assay parameters were optimized, such as decreasing the primer concentration, reducing the incubation time of the LAMP reaction, testing different published primer sets, and ...
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A New Real Time PCR Assay for Improved Detection of the Parasite Babesia microti
... Babesiosis is an emerging zoonosis with important public health implications, as the incidence of the disease has risen dramati- cally over the past decade. Because the current gold standard for detection of Babesia is ...
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First report on Babesia cf microti infection of red foxes (Vulpes vulpes) from Hungary
... Canine babesiosis caused by the large Babesia species, B. canis is endemic in Hungary [28,29]. To date, only one report of a small Babesia infection based on microscopic observation which caused babesiosis in two dogs in ...
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Detection and identification of Candida species in experimentally infected tissue and human blood by rRNA specific fluorescent in situ hybridization
... out cultivation (1, 3). Using a probe that binds to a target region in the 18S rRNA of C. albicans and Candida tropicalis, we have previously demonstrated that this method is applica- ble for the detection ...
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A review of piroplasmid infections in wild carnivores worldwide: importance for domestic animal health and wildlife conservation
... Babesia lotori [42]. However, in the last years, molecular analyses carried out in North America and Japan (where this species was introduced) identified several sequences corresponding with two or more species of ...
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Molecular evidence of Sarcocystis nesbitti in water samples of Tioman Island, Malaysia
... Following species identification, the results were reap- praised by comparing the 21 cloned sequences with another 31 sequences of the 18S rRNA gene from vari- ous other Sarcocystis species obtained from ...
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Improved molecular detection of Babesia infections in animals using a novel quantitative real time PCR diagnostic assay targeting mitochondrial DNA
... subunit rRNA gene frag- ments (lsu5 and lsu4) conserved among Babesia ...genus 18S rDNA qPCR (18S qPCR), utilized by the VBDDL-NCSU for Babesia molecular diagnostic detection, was used to amplify a ...
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A Clinicomycological study of Onychomycosis, its Antifungal Susceptibility Pattern and Rapid Detection of Trichophyton Genus from Nail Samples by PCR Analysis of 18S rRNA Gene Internal Transcribed Spacer Region.
... CLINICOMYCOLOGICAL STUDY OF ONYCHOMYCOSIS, ITS ANTIFUNGAL SUSCEPTIBILITY PATTERN AND RAPID DETECTION OF TRICHOPHYTON GENUS FROM NAIL SAMPLES BY PCR ANALYSIS OF 18S rRNA GENE INTERNAL TRANSCRIBED SPACER ...
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New molecular settings to support in vivo anti malarial assays
... concentration was detected and expressed as log10 para- sites/µl. Only Ct values below 40 and duplicate Ct values with standard deviation within ±1.5 Ct were considered. The mean LOD values are given in Table 4 and ...
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Isolation, cultivation and molecular characterization of a new Trypanosoma equiperdum strain in Mongolia
... Additional file 3: Figure S1. The phylogenetic tree of the 18S rRNA and ITS region. A phylogenetic analysis was performed using the T. equiperdum IVM-t1, SITB818, STIB841, STIB842, BoTat1.1, T. evansi ...
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Effects of gene therapy on muscle 18S rRNA expression in mouse model of ALS
... stimulate rRNA tran- scription by direct transcriptional regulation as well as to activate synthesis of ribosomal proteins by stimula- tion of Akt/PI3K survival pathway ...I-dependent rRNA transcription ...
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RNase L-Independent Specific 28S rRNA Cleavage in Murine Coronavirus-Infected Cells
... 28S rRNA cleavage in cells infected with the murine coronavirus mouse hepatitis virus ...28S rRNA cleavage occurred as early as 4 h postinfection ...28S rRNA with increasing times of infection; ...
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