18S rRNA gene
Specific detection of fungal pathogens by 18S rRNA gene PCR in microbial keratitis
... The most common initial laboratory procedure done in diagnosis of fungal keratitis is microscopic examination. In this study, a positive microscopic examination was observed in 30% (9) of patients. The percentage of ...
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Molecular analysis of 18S rRNA gene of Cryptosporidium parasites from patients living in Iran, Malawi, Nigeria and Vietnam
... the 18S rRNA gene in Cryptosporidium for exact identification of parasites isolated from Iran, Malawi, Nigeria and ...this gene target to a phylogenetic analysis of ...
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Molecular detection of 18s rrna gene of candida albicans isolated from pregnant women with clinical diagnosis of vulvovaginitis
... The 18S rRNA gene is a multi-copy gene that is slowly evolving and highly conserved among fungi, making it an attractive target for the detection of Candida yeast in clinical ...by 18S ...
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Use of 18S rRNA Gene Based PCR Assay for Diagnosis of Acanthamoeba Keratitis in Non Contact Lens Wearers in India
... An 18S rRNA gene-based PCR test, highly specific for the genus Acanthamoeba, has recently been reported in the molecular diagnosis of Acanthamoeba ...
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Fluorescent Oligonucleotide Probes for Clinical and Environmental Detection of Acanthamoeba and the T4 18S rRNA Gene Sequence Type
... described 18S rRNA gene (rDNA) sequence types of Acanthamoeba (Table 1) plus the bacteria, human cells, and other amoebae described above were ...
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Detection of Four Plasmodium Species in Blood from Humans by 18S rRNA Gene Subunit Based and Species Specific Real Time PCR Assays
... the 18S rRNA gene of the genus Plasmodium was designed; the primer set was polymorphic enough internally to design four species- specific probes for ...the 18S screening ...between 18S ...
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Molecular Analysis of the 18S rRNA Gene of Cryptosporidium Parasites from Patients with or without Human Immunodeficiency Virus Infections Living in Kenya, Malawi, Brazil, the United Kingdom, and Vietnam
... Sequencing and phylogenetic analysis. The internal (secondary) fragment of approximately 840 bp was purified with a GenElute commercial kit (Sigma Chemicals, Watford, United Kingdom) according to the manufacturer’s ...
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A Clinicomycological study of Onychomycosis, its Antifungal Susceptibility Pattern and Rapid Detection of Trichophyton Genus from Nail Samples by PCR Analysis of 18S rRNA Gene Internal Transcribed Spacer Region.
... CLINICOMYCOLOGICAL STUDY OF ONYCHOMYCOSIS, ITS ANTIFUNGAL SUSCEPTIBILITY PATTERN AND RAPID DETECTION OF TRICHOPHYTON GENUS FROM NAIL SAMPLES BY PCR ANALYSIS OF 18S rRNA GENE INTERNAL TRANSCRIBED ...
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PCR-Denaturing gradient gel electrophoresis profiling of inter- and intraspecies 18S rRNA gene sequence heterogeneity is an accurate and sensitive method to assess species diversity of arbuscular mycorrhizal fungi of the genus Gigaspora
... The genus Gigaspora represents an ecologically and eco- nomically (4, 42) important group within the Glomeromycota, and numerous studies have been dedicated to identify species within this genus and to study their ...
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High resolution melting analysis of the 18S rRNA gene for the rapid diagnosis of bovine babesiosis
... HRM assays were performed using a Rotor-Gene Q6000 Real-Time PCR system (Qiagen, Sydney, Australia). Each reaction was performed in a total volume of 20 μl con- taining 10 μl of Forget-Me-Not ™ qPCR Master Mix ...
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PHYLOGENETIC ANALYSIS OF FRESHWATER MUSSEL BY Corbicula regularis 18S rRNA GENE SEQUENCING
... using 18S ribosomal RNA gene markers. Genomic DNA was extracted and 18S rRNA gene was amplified by universal ...that 18S rRNA gene sequences of ...of 18S ...
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Applied Genomics: Data Mining Reveals Species Specific Malaria Diagnostic Targets More Sensitive than 18S rRNA
... the 18S rRNA gene targets. Historically, this gene has served as the best target for diagnostic ...than 18S rRNA when utilized in a single-step ...nested 18S rRNA ...
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18S rRNAis a reliable normalisation gene for real time PCR based on influenza virus infected cells
... expression, 18S rRNA was the most stable among all the genes tested in HBECs, PTECs, and chicken and duck lung cells 24h following infection with various influenza virus subtypes (Table ...between ...
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18S rRNA is a reliable normalisation gene for real time PCR based on influenza virus infected cells
... expression, 18S rRNA was the most stable among all the genes tested in HBECs, PTECs, and chicken and duck lung cells 24h following infection with various influenza virus subtypes (Table ...between ...
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Determination of the etiology of presumptive feline leprosy by 16S rRNA gene analysis
... 16S rRNA genes of ...16S rRNA gene nucleotide position 443 ...16S rRNA sequence isolated from the NI cat leprosy specimen could only be ascribed to ...
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Species Specific Identification of Campylobacters by Partial 16S rRNA Gene Sequencing
... Present guidelines suggest that 3% variation between two rDNAs is the threshold at which two strains may be considered to represent distinct species (7, 15, 24, 44). By taking this value of sequence variation into ...
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Identification of Pathogenic Nocardia Species by Reverse Line Blot Hybridization Targeting the 16S rRNA and 16S 23S rRNA Gene Spacer Regions
... 16S rRNA gene sequence analysis is employed most often for the definitive identification of Nocardia species, alternate molecular methods and polymorphisms in other gene targets have also enabled ...
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The distribution, diversity, and importance of 16S rRNA gene introns in the order Thermoproteales
... locations, rather than with related genera. For example, the Thermoproteales-like homing endonuclease encod- ing genes at loci 781, 1093, and 1213 (shown in Fig. 5a) formed clades corresponding to sequences from Japan, ...
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DETECTION OF BACTERIA BY AMPLIFYING THE 16S rRNA GENE WITH UNIVERSAL PRIMERS AND RFLP
... Background: There is a conserved portion in the 16S rRNA gene of bacteria which can be amplified by the universal PCR method. This fragment is 996 bp in length. In this method, only one set of universal ...
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Catheter Related Microbacterium Bacteremia Identified by 16S rRNA Gene Sequencing
... 16S rRNA gene analysis confirmed that our two isolates were Mi- crobacterium species, the 16S rRNA genes in different Mi- crobacterium species are highly ...16S rRNA sequence identity may not ...
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