2D and 3D cell culture
Cellular effects of paclitaxel-loaded iron oxide nanoparticles on breast cancer using different 2D and 3D cell culture models
... The present work was performed in fulfillment of the requirements for obtaining the degree “Dr. med.” (Stephan Lugert). This study was supported by the Deutsche Forsc- hungsgemeinschaft (SPP1681: Grant Numbers: AL ...
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Three dimensional cell culture: The missing link in drug discovery
... support 2D cultures that, arguably, do not represent the in vivo environment ...a cell suspension is ...enabling cell–cell ...for cell– matrix ...the cell spheroids ...support ...
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Matrigel® as 3D culture medium: 2D vs. 3D changes in proliferation and ultrastructure
... single cell type to grow in a monolayer on a glass-bottomed Petri dish (MatTek ...Thus, 3D matrices that support multilayer systems are valuable tools for the following: furthering the understanding of ...
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The 3D Cell Culture System in the Study of Tumor Applications and Prospects
... years, 3D fine cell culture technology have shown incompara- ble advantage in the field of drug research and development, especially the eval- uation of drug safety [12] and the study of new drug ...
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MnO2-gated Nanoplatforms with Targeted Controlled Drug Release and Contrast-Enhanced MRI Properties: from 2D Cell Culture to 3D Biomimetic Hydrogels
... from 2D data to in vivo during preclinical studies may therefore be extremely time-consuming and ...architecture, cell-cell interaction and hypoxia should provide highly useful tumor tissue in vitro ...
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Differentiation of Menstrual Blood Derived Stem Cell (MensSCs) to Hepatocyte Liked Cell on Three Dimensional Nanofiberscaffold: Poly Caprolacton (PCL)
... stem cell markers [25] such as CD 44, CD 90, CD 73, and CD 105 and low expression of hematopoietic markers, CD 34 and CD 45, confirmed this source of stem cell as multipo- tent stromal ...promote ...
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Chick tendon fibroblast transcriptome and shape depend on whether the cell has made its own collagen matrix
... in 2D, in tendon in vivo and in fibrin gel- and collagen gel-based tendon-like ...in culture, are likely to explain some of these ...in culture mimicked a late aspect of tendon ...the 3D ...
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Investigating NF-kappa B signaling in lung fibroblasts in 2D and 3D culture systems
... in 3D had a dose dependent response to all TNF-α concentrations albeit the level of NF-κB activation in 3D cultures was lower than 2D under the same experimental conditions ...in 3D exhibit a ...
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Three-dimensional culture of MSCs produces exosomes with improved yield and enhanced therapeutic efficacy for cisplatin-induced acute kidney injury
... 3D culture platform presents more advantages over conventional 2D ...this 3D culture system can culture a multitude of cells (estimated to be in the order of 10 9 cells) within a ...
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A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics
... in cell morphology in 3D collagen scaffold cultures as compared to 2D ...in culture, U87 and primary glioma cells were fixed, dehydrated and embedded in paraffin for H&E staining or dried ...
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2193-1801-3-80.pdf
... on 3D gel substrates. In 1985, Sertoli cell and germ cell survival and differentiation were accomplished by using a 3D ...using 3D systems (Benton et al. 2009). 3D systems ...
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Short term ex vivo expansion of circulating head and neck tumour cells
... low cell confluency (10–20%). There were sporadic areas of growth in 2D with high cell populations in those select areas reflecting cells which may have proliferated more ...in 3D, and in all ...
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Induction of HOXA9 expression in three-dimensional organotypic culture of the Claudin-low breast cancer cells
... in 2D culture, but robustly induced in lrECM 3D culture relative to the Luminal MCF-7 and T47D cells (Figure 1, Supplementary Materials Table ...lrECM 3D culture [43]. These ...
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Differentiation of human embryonic stem cells into hepatocytes in 2D and 3D culture systems in vitro
... in culture with exogenous growth ...with 2D culture, earlier gene expression patterns of ALB and G6P and higher detection levels of urea and AFP in 3D culture systems suggest that ...
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In silico characterization of cell–cell interactions using a cellular automata model of cell culture
... of cell population ...include cell–cell adhesion effects to simulate cultured cells in 2D and 3D [19, 20, ...the cell–cell interaction properties of cultured ...the ...
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Biologic variability of human foreskin fibroblasts in 2D and 3D culture: implications for a wound healing model
... The data in this report may have been variable because the biology was variable. This supposition is supported by the inter-strain variability seen in all four Figures, and the fact that the statistical analysis ...
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Use of hydrogel scaffolds to develop an in vitro 3D culture model of human intestinal epithelium
... The human intestinal cell lines: Caco-2 and HT29-MTX cells have been used.. extensively in 2D and 3D cell cultures as simple models of the small intestinal.[r] ...
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Epigallocatechin-3-gallate (EGCG) inhibits the migratory behavior of tumor bronchial epithelial cells
... inhibit cell migration or inva- sion in liver cancer cells [5], glioblastoma cells [3], vascu- lar smooth muscle cells [35,36], pancreatic stellate cells [37] or during angiogenesis ...the cell migration ...
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Differences of statin activity in 2D and 3D pancreatic cancer cell cultures
... cancer cell lines BxPC-3, MIA PaCa-2, and PANC-1 were obtained from the American Type Culture Collection (ATCC, Manassas, VA, ...This cell line was origi- nally purchased from ...PANC-1 cell ...
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Expansion of human cord blood hematopoietic stem/progenitor cells in three-dimensional Nanoscaffold coated with Fibronectin.
... in 3D scaffold, it seems that total colonies in 3D culture system were higher than those of 2D culture ...in 3D culture system was higher than routinely cell ...
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