3′→5′-exonuclease activity
CiAPEX2 and CiP0, candidates of AP endonucleases in Ciona intestinalis, have 3′-5′ exonuclease activity and contribute to protection against oxidative stress
... or 3 ′ -phosphodiesterase activity, these two purified proteins exhibited 3 ′ -5 ′ exonuclease ...This 3 ′ -5 ′ exonuclease activity was sensitive to ...
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Molecular interactions of Escherichia coli ExoIX and identification of its associated 3 '-5 ' exonuclease activity
... and the GST–ExoIX fusion proteins were able to bind to E. coli SSB. SSB forms a tetramer and is known to bind tightly to single-stranded DNA within the E. coli cell. ExoIX appears to be able to bind to the SSB tetramer ...
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The archaeal RecJ like proteins : nucleases and ex nucleases with diverse roles in replication and repair
... possesses 5’→3’ ssDNA-specific exonuclease activity (like TkoGAN) while TaRecJ2 has 3’→5’ exonuclease activity on RNA and ssDNA, with RNA being the preferred ...
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The Enhanced DNA Replication Fidelity of a Mutant Herpes Simplex Virus Type 1 DNA Polymerase Is Mediated by an Improved Nucleotide Selectivity and Reduced Mismatch Extension Ability
... enzymatic activity and DNA replication ...the 3 ⬘ -5 ⬘ exonuclease activity are the key steps in maintaining DNA replication fi- delity (reviewed in reference ...
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p53 in the mitochondria, as a trans-acting protein, provides error-correction activities during the incorporation of non- canonical dUTP into DNA
... of 5’-fluorouracil ...intrinsic 3′→5′ exonuclease activity can diminish the polymerization ...an exonuclease/proofreader for polymerase γ by either decreasing the incorporation ...
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Functions of Multiple Exonucleases Are Essential for Cell Viability, DNA Repair and Homologous Recombination in recD Mutants of Escherichia coli
... ExoI activity in both recombination assays, indicating that exonu- cleases are not always synergistic in ...59–39 exonuclease activity of RecJ, ExoVII is required for recombination and repair, ...
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Amino Acid Residues Critical for the Interaction between Bacteriophage T7 DNA Polymerase and Escherichia coli Thioredoxin
... gene 5, and its processivity factor, ...gene 5. Six different mutations in T7 gene 5 are individually necessary and sufficient to com- pensate for the G74D thioredoxin ...the 3 9 to 5 9 ...
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Squaramide Based 5' Phosphate Replacements Bind to the DNA Repair Exonuclease SNM1A
... a 3’-Cy3 fluorophore 1a or a free 3’-OH group 1b were synthesized to allow for visualization where ...a 3:2 mixture of 20mer to 21mer 1a and a 2:3 mixture of 20mer to 21mer ...observed ...
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Functional insight into Maelstrom in the germline piRNA pathway: a unique domain homologous to the DnaQ-H 3'–5' exonuclease, its lineage-specific expansion/loss and evolutionarily active site switch
... nuclease-like activity from three main findings: Firstly, the novel MAEL-specific domain is defined by a set similar sequence segments (related via a few PSI-BLAST searches) with a conserved motif involving ...
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Baculovirus Alkaline Nuclease Possesses a 5′→3′ Exonuclease Activity and Associates with the DNA-Binding Protein LEF-3
... exonuclease (Red␣), which degrades double- stranded DNA (dsDNA) from the 5⬘ ends, producing 3⬘ over- hangs which serve as intermediates in recombination ... exonuclease forms a ...
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Herpes Simplex Virus UL12.5 Targets Mitochondria through a Mitochondrial Localization Sequence Proximal to the N Terminus
... The herpes simplex virus type 1 (HSV-1) gene UL12 encodes a conserved alkaline DNase with orthologues in all herpesviruses. The HSV-1 UL12 gene gives rise to two separately promoted 3 ⴕ coterminal mRNAs which ...
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The CRISPR associated protein Cas4 Is a 5' to 3' DNA exonuclease with an iron sulfur cluster
... nuclease activity for Sso1391 was detected under any condition tested, probably because it forms a functional complex with Cas1 and Cas2 in vivo ...nuclease activity was derived from the Sso0001 protein, a ...
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Herpes Simplex Virus 1 DNA Polymerase RNase H Activity Acts in a 3′-to-5′ Direction and Is Dependent on the 3′-to-5′ Exonuclease Active Site
... detectable 3=-to-5= exonuclease ...detectable 3=-to-5= exonuclease activity on our hairpin ...detected 3=-to-5= exonuclease activity associated ...
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Evidence for Both 3’ and 5’ Single-Strand DNA Ends in Intermediates in Chi-Stimulated Recombination In Vivo
... This idea is supported by our finding here that three different en- zymes with single-strand dependent exonuclease activity of 3‘ polarity, of 5’ polarity and of both polarit[r] ...
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The 3′-to-5′ Exonuclease Activity of Vaccinia Virus DNA Polymerase Is Essential and Plays a Role in Promoting Virus Genetic Recombination
... E9 3 ⬘ -to-5 ⬘ exonuclease ...proofreading activity to catalyze a presynaptic step in genetic ...proofreading activity of E9 is used to remove CDV from the 3 ⬘ ends of the DNA ...
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Detection and Quantitation of Human Papillomavirus by Using the Fluorescent 5′ Exonuclease Assay
... Plasmids containing HPV types 16, 18, 31, 33, and 35 were used as positive controls and to estimate the sensitivity of the assay (1, 9, 10, 24). The plasmids with integrated HPV were transformed into One Shot cells (INV ...
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Purification and properties of Epstein-Barr virus DNase expressed in Escherichia coli.
... In the presence of high KCI concentrations, only exonuclease activity could be detected from the recombinant DNase, while endonuclease activity was observed from the LCL-extracted enzyme[r] ...
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A fully 5'-CG-3' but not a 5'-CCGG-3' methylated late frog virus 3 promoter retains activity.
... the 5 9 upstream region of this gene, we had earlier assessed the pattern of complete 5 9 -CG-3 9 methylation by using the genomic sequencing method ...all 5 9 -CG-3 9 (enhanced ...
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IN VITRO ANTHELMINTIC ACTIVITY OF 3 (3 CHLOROPHENYL) 5 PHENYL 4, 5 DIHYDRO 1,2 OXAZOLE DERIVATIVES
... from 3- Chloro acetophenone with hydroxyl amine hydro chloride, small amount of sodium acetate and ethanol All the synthesised compounds were characterized by using the IR, H 1 NMR ...Anthelmintic activity ...
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The REC1 Gene of Ustilago maydis, Which Encodes a 3′ → 5′ Exonuclease, Couples DNA Repair and Completion of DNA Synthesis to a Mitotic Checkpoint
... The partial suppression of radiation- and hydroxy- urea-induced killing of recl-l by arresting cells in G2 with MBC and the decrease in the G2 phase cell popula- tion [r] ...
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