3-glucanase
Cloning and Bioinformatics Analysis of Rosa rugosa β 1,3 Glucanase Gene (RrGlu)
... The Rosa rugosa is a famous traditional Chinese flower. It is fragrant as well as resistant to cold, drought, pest, disease, salt, and alkali [1]-[3]. However, due to its short bloom phase and uniform color and ...
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Functional analysis of conserved aromatic amino acids in the discoidin domain of Paenibacillus β-1,3-glucanase
... The 190-kDa Paenibacillus β-1,3-glucanase (LamA) contains a catalytic module of the glycoside hydrolase family 16 (GH16) and several auxiliary domains. Of these, a discoidin domain (DS domain), present in both ...
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Recombinant Arthrobacter β 1, 3 glucanase as a potential effector molecule for paratransgenic control of Chagas disease
... In this study, we investigate the potential role of endoglucanases as anti-trypanosomal agents. The surface of T . cruzi is covered by a thick coat of mucin-like glyco- proteins. Many of these glycoproteins are ...
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Expression Vector Construction and Genetic Transformation of Rosa rugosa β l,3 Glucanase Gene (RrGlu)
... Under normal conditions, the expression of β-1,3-glucanase gene is pretty low in plants. When the plant is induced by pathological or relative conditions, the enzyme accumulates rapidly and its expression activity ...
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The cell wall localized atypical β 1,3 glucanase ZERZAUST controls tissue morphogenesis in Arabidopsis thaliana
... -1,3 glucanase suggested to degrade the cell wall polymer ...-1,3 glucanase ZERZAUST acts in a non-cell-autonomous manner and is required for cell wall organization during tissue ...
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Screening and identification of resistance related proteins from apple leaves inoculated with Marssonina coronaria (EII. & J. J. Davis)
... beta-1, 3-glucanase (L17) have a good relation with the abundance of the encoded protein ’ s accumulation, however, the mRNA abundance of thaumatine-like protein (L22) and ascorbate peroxidase (L28) are not ...
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Genetic transformation of Tomato with three pathogenesis-related protein genes for increased resistance to Fusarium oxysporum f.sp. lycopersici
... β-1,3- glucanase which was well examined at physiological and molecular levels, plays an extensive role in defense reactions (Simmons et al, ...β-1,3- glucanase is able to decompose the available glucan on ...
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β-Glucanase Eng2 Is Required for Ascus Wall Endolysis after Sporulation in the Fission Yeast Schizosaccharomyces pombe
... of incubation in the wild-type strain, and a similar value was observed for the eng2 ⌬ mutant. Microscopic inspection of spores incubated for 24 h in sporulation medium suggested that the spores produced by the eng2 ⌬ ...
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... cotyledons of bean plants than hypocotyl and a significant increase in all cellular fractions of PO, β-1,3-glucanases and chitinase compared with diseased and control plants. β-1,3-glucanase activity was also ...
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Revisiting the Cellulosimicrobium cellulans yeast-lytic β-1,3-glucanases toolbox: A review
... β-1,3- glucanase LamA from the archeon Pyrococcus furiosus (GenBank accession number AF013169) [34], 49 % iden- tity, the β-1,3-glucanase from Strongylocentrotus purpuratus (sea urchin), (GenBank accession ...
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Rice induced resistance, elicited by β glucanase from Peanopacillus polymxa, in controlling blast disease
... The rice variety, Sakha 101 was used in this study. The rice seeds were germinated for 3 days at room temperature. The seedlings were then planted in 15-cm-diameter pots containing sterile moist soil (clay: sand, ...
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Autolysis of Pichia pastoris induced by cold
... The production of recombinant biopharmaceutical proteins is a multi‑billion dollar market. Protein recovery repre‑ sents a major part of the production costs. Pichia pastoris is one of the microbial systems most used for ...
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Two Novel Techniques for Determination of Polysaccharide Cross-Links Show that Crh1p and Crh2p Attach Chitin to both β(1-6)- and β(1-3)Glucan in the Saccharomyces cerevisiae Cell Wall
... adsorb all of them, except for the free chitin. In fact, when the experiment was carried out, ca. 40% of the radioactivity re- mained in the supernatant, an amount similar to that of free chitin, as determined by the ...
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Systemic Activation of Defensive Enzymes and Protection in Tobacco Plantlets against Phytophthora nicotianae Induced by Oligosaccharins
... The dynamic behaviour of β 1 - 3 glucanase activity was about similar than PAL (Figure 3). A biphasic be- haviour with the amino compounds and just one phase behaviour for OGAS elicitor, peaking at ...
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IDENTIFICATION OF Trichoderma harzianum T3.13 AND ITS INTERACTION WITH Neoscytalidium dimidiatum U1, A PATHOGENIC FUNGUS ISOLATED FROM DRAGON FRUIT (Hylocereus polyrhizus) IN MALAYSIA
... β-1, 3-glucanases and proteases (López-Mondéjar et ...β-1, 3-glucanase enzymes can result in the suppression of plant pathogen activities ...α-1, 3-glucanase (Sanz et ...α-1, ...
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Proteins Potentially Involved in Immune Evasion Strategies in Sporothrix brasiliensis Elucidated by Ultra-High-Resolution Mass Spectrometry
... Mass spectrometry. Tryptic digests (peptides) were analyzed by online nanoflow LC using an Easy-nLC II system (Thermo Fisher Scientific). Peptides (5- l injection) were separated with a 60-min gradient (0 to 35% buffer B ...
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Characterization of Exo 1, 4 β glucanase produced from Tricoderma Viridi MBL through solid state bio processing of orange peel waste
... day of incubation at pH 5.5 and 30˚C. T. viridi showed high levels of Exo 1, 4-β glucanase production during solid state bio-processing of orange peel waste. In the present study, an eco-friendly procedure has ...
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The Eng1 β Glucanase Enhances Histoplasma Virulence by Reducing β Glucan Exposure
... In contrast to ␣-glucan, which minimizes -glucan exposure by a concealment mechanism, the Eng1 glucanase acts by removal of exposed -glucans. While we cannot rule out other substrates for Eng1, given that the ...
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RSM/ANN based optimized recovery of phenolics from mulberry leaves by enzyme-assisted extraction
... Morus alba leaves (MAL) were collected from village Khai Kotli district Jhelum, Punjab in Pakistan. After drying, the ground powder was stored at – 4°C until analysed (Qadir et al. 2018). Commercial enzyme mixtures, such ...
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Enhanced saccharification of rice straw by overexpression of rice exo-glucanase
... has been greatly increased by breeding of crop plants, breeding of material plants is also indispensable for effi- cient production of biofuels. For this purpose a number of experiments have been carried out to ...
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