3C-like protease
In silico Repositioning for Dual Inhibitor Discovery of SARS-CoV-2 (COVID- 19) 3C-like Protease and Papain-like Peptidase
... papain-like protease (PLpro; corresponding to nsp3) and a main protease (Mpro), 3C‑like protease (3CLpro; corresponding to ...
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3C-like protease of rabbit hemorrhagic disease virus: identification of cleavage sites in the ORF1 polyprotein and analysis of cleavage specificity.
... RHDV 3C-like protease expressed in ...fused protease-polymerase protein generated in this assay does not cleave the EG at positions 1108 and 1109 as efficiently as it does the cleavage site at ...
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Polyprotein processing in Southampton virus: identification of 3C-like protease cleavage sites by in vitro mutagenesis.
... viral protease is produced following translation of a region located between NheI (nucleotide 3052) and SphI (nucleotide 4056), resulting in rapid cleavage of a large precursor ...viral 3C-like ...
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Identification of Active-Site Amino Acid Residues in the Chiba Virus 3C-Like Protease
... viral 3C proteases, whereas similarity was relatively high around this sequence motif ...virus 3C-like ...virus 3C-like protease. (A) Alignment of the 3C-like ...
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Genetic Screen for Monitoring Severe Acute Respiratory Syndrome Coronavirus 3C-Like Protease
... -Gal–SCoV 3C-like protease construct and the ...the protease was induced with IPTG for 1 h, and the cells were infected with for three additional ...-Gal–SCoV 3C-like pro- ...
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Coronaviruses Resistant to a 3C-Like Protease Inhibitor Are Attenuated for Replication and Pathogenesis, Revealing a Low Genetic Barrier but High Fitness Cost of Resistance
... pain-like protease (PLP) and the 3C-like protease (3CLpro, also known as the main ...Numerous protease inhibitors have been synthe- sized and identified to inhibit ...
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Porcine Epidemic Diarrhea Virus 3C-Like Protease Regulates Its Interferon Antagonism by Cleaving NEMO
... PEDV-encoded 3C-like protease, nsp5, is an IFN antagonist that proteolytically cleaves the nu- clear transcription factor kappa B (NF- B) essential modulator (NEMO), an essential adaptor bridging ...
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Characterization of an Alphamesonivirus 3C-Like Protease Defines a Special Group of Nidovirus Main Proteases
... N idovirales (families Coronaviridae, Roniviridae, Mesoniviri- dae, and Arteriviridae) are positive-sense, single-stranded RNA viruses that infect a wide range of vertebrate and invertebrate hosts (1–7). Members of the ...
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Porcine Epidemic Diarrhea Virus 3C-Like Protease-Mediated Nucleocapsid Processing: Possible Link to Viral Cell Culture Adaptability
... ABSTRACT Porcine epidemic diarrhea virus (PEDV) causes severe diarrhea and high mortality rates in newborn piglets, leading to massive losses to the swine industry worldwide during recent epidemics. Intense research ...
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Mouse Hepatitis Virus 3C-Like Protease Cleaves a 22-Kilodalton Protein from the Open Reading Frame 1a Polyprotein in Virus-Infected Cells and In Vitro
... min, similar to the time of first appearance during pulse-label- ing, indicating that at least some cleavage of p1a-22 occurred as soon as translation was completed (Fig. 3B, lanes 12 to 13). Pulse-chase labeling of MHV ...
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Mechanism for Controlling the Dimer-Monomer Switch and Coupling Dimerization to Catalysis of the Severe Acute Respiratory Syndrome Coronavirus 3C-Like Protease
... the continuous sedimentation coefficient distribution [c(s)] model (Fig. 2). Moreover, based on the continuous molar mass distribution [c(M)] model, the molecular masses of the R298A mutant at different concentrations ...
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Identification and characterization of a 3C-like protease from rabbit hemorrhagic disease virus, a calicivirus.
... On the other hand, replacement with Ser, which maintains the nucleophilic character of Cys but does not have similar structural functions such as the ability to form disulfide bridges, y[r] ...
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Molecular Characterization of a Porcine Enteric Calicivirus Genetically Related to Sapporo-Like Human Caliciviruses
... helicase, 3C-like protease, 3D RNA-dependent RNA polymer- ase, and a structural protein of 544 aa ...(GPPGIGKT), 3C protease (GDCG), and RNA-dependent RNA polymerase (GLPSG and YGDD) ...
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Highly Conserved Configuration of Catalytic Amino Acid Residues among Calicivirus-Encoded Proteases
... calicivirus 3C-like proteases. (A to D) Structural models of calicivirus 3C-like protease domains of the SaV Mc10 (A), FCV F4 (B), and RHDV FRG (C) strains and crystal structure of the ...
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Efficient Cleavage of Ribosome-Associated Poly(A)-Binding Protein by Enterovirus 3C Protease
... Poliovirus (PV) causes a rapid and drastic inhibition of host cell cap-dependent protein synthesis during infection while preferentially allowing cap-independent translation of its own genomic RNA via an internal ...
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Serological Evidence of Human Klassevirus Infection
... of 3C protease. The klassevirus 3C protease gene (nucleotides [nt] 5825 to 6409 from strain 2394-01; ...klassevirus 3C gene was transformed into Escherichia coli BL21(DE3)LysS/pRIL and ...
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Chlamydial Protease Like Activity Factor and Type III Secreted Effectors Cooperate in Inhibition of p65 Nuclear Translocation
... chlamydial protease- like activity factor (CPAF) is a conserved serine protease secreted into the host cytosol of infected cells that is thought to play an important role in immune ...
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Virtual screening and molecular docking of Anti-Antileishmanial for selected pharmacophore for visceral Leishmaniasis
... Cathepsin like cysteine protease is an important target protein for the identification of novel lead compound of ...Cathepsin like cysteine protease representing a major component of the ...
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Identification of active-site residues in protease 3C of hepatitis A virus by site-directed mutagenesis.
... and 3ABC. Also, during the final 3-h interval of IVTT, peptide 3BC accumulated. Taking this pattern of peptide synthesis and proteolytic processing as the standard for events in IVTT of P3 polyprotein, by comparison ...
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Structural Basis for Antiviral Inhibition of the Main Protease, 3C, from Human Enterovirus 93
... ample, EV-71 of HEV-A caused serious complications—en- cephalitis and myocarditis—and death during epidemics of hand, foot, and mouth disease in Asia in 1997, 1998, 2000, and 2008 (44). The disease manifestation of acute ...
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