3C protease
Biochemical characterization of recombinant Avihepatovirus 3C protease and its localization
... DHAV 3C protease could localize to the nucleus of DEF cells, presumably due to the degradation of specific ...the 3C protease in other pi- cornaviruses. The wild-type (WT) 3C ...
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Efficient Cleavage of Ribosome-Associated Poly(A)-Binding Protein by Enterovirus 3C Protease
... the 3C cleavage product was observed in total lysate but not in the ribosome-enriched fraction, similar to in vitro results ...of 3C cleavage product in the ribosome-enriched compartment sug- gest that most ...
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3C Protease of Enterovirus D68 Inhibits Cellular Defense Mediated by Interferon Regulatory Factor 7
... 3C pro cleaves IRF7 at Gln-167 and Gln-189. Since IRF7 cleav- age produced two 20-kDa fragments representing the N terminus and one 40-kDa fragment representing the C terminus (Fig. 5A), we inferred that the IRF7 ...
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Polyprotein processing in cis and in trans by hepatitis A virus 3C protease cloned and expressed in Escherichia coli.
... 5242 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest To determine the P3 region protein-processing sites cleaved by the hepatitis A virus 3C protease, a nested set of co[r] ...
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Foot-and-Mouth Disease (FMD) Virus 3C Protease Mutant L127P: Implications for FMD Vaccine Development
... by 3C(L127P) collectively would support the conclusion that this region of the 3C protease can play a critical role in substrate specificity despite being distal to the active ...by 3C(L127P) ...
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Foot-and-Mouth Disease Virus 3C Protease Induces Cleavage of Translation Initiation Factors eIF4A and eIF4G within Infected Cells
... FMDV 3C protease ...FMDV 3C as discussed above [Ross-Smith and Belsham, unpublished ...FMDV 3C, the protein segments may not dissociate within the cell and the fragments may continue to ...
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Cleavage of small peptides in vitro by human rhinovirus 14 3C protease expressed in Escherichia coli.
... Therefore, synthetic substrates designed to mimic authentic viral polyprotein cleavage sites were used to determine whether purified 3C protease also was capable of specifically cleaving[r] ...
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Inhibition of enterovirus 71 replication and viral 3C protease by quercetin
... EV71 3C pro is a specific protease that recognizes peptide substrates containing a Q-G junction ...μM 3C protease in 50 mM Tris-HCl, 200 mM NaCl, 2 mM DTT, and different concentrations of ...
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3C Protease of Enterovirus 68: Structure-Based Design of Michael Acceptor Inhibitors and Their Broad-Spectrum Antiviral Effects against Picornaviruses
... the 3C pro of rhinovirus 2, as well as to that of ...EV68 3C pro between the cor- responding enzymes of rhinoviruses on the one hand and classical enteroviruses on the other prompted us to use the crystal ...
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Foot-and-Mouth Disease Virus 3C Protease Cleaves NEMO To Impair Innate Immune Signaling
... (NF- B) essential modulator (NEMO) and inhibitors of B ki- nase (IKK)-related kinases, such as IKK- ␣ , -  , and - ε , as well as TANK-binding kinase 1 (TBK1), are activated, leading to the ac- tivation of various ...
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Crystal Structures of Enterovirus 71 3C Protease Complexed with Rupintrivir Reveal the Roles of Catalytically Important Residues
... centrated to ⬃10 mg/ml prior to the addition of rupintrivir (TRC, Inc.) dissolved in methanol. The final molar ratio of protease to inhibitor was 1:5. After a brief incubation on ice, the mixtures were clarified ...
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Inducible expression of encephalomyocarditis virus 3C protease activity in stably transformed mouse cell lines.
... At various times postinfection, the cells were washed with PBS and lysates were prepared for protease activity assays as described or for immune precipitation as described in Materials a[r] ...
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Structural and Mutagenic Analysis of Foot-and-Mouth Disease Virus 3C Protease Reveals the Role of the β-Ribbon in Proteolysis
... yielded essentially wild-type levels of activity. Substitution of a less apolar residue at position 142 (Ala, Val, or Thr) gave an intermediate level of activity. These data, which provide the first functional test of ...
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Foot-and-Mouth Disease Virus 3C Protease Induces Fragmentation of the Golgi Compartment and Blocks Intra-Golgi Transport
... (FMDV) 3C pro and that this requires the protease activity of 3C pro .... 3C pro caused fragmentation of early, medial, and late Golgi compart- ments, but the most marked effect was on early ...
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Enterovirus 68 3C Protease Cleaves TRIF To Attenuate Antiviral Responses Mediated by Toll-Like Receptor 3
... of EV68 and was cloned into the XhoI and BamHI sites of the pEGFPC1 vector, resulting in green fluorescent protein (GFP) fusion proteins. The GFP-3C variants and TRIF mutants were constructed by using Pfu DNA ...
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Encephalomyocarditis virus 3C protease: efficient cell-free expression from clones which link viral 5' noncoding sequences to the P3 region.
... Translation of encephalomyocarditis virus EMC RNA in rabbit reticulocyte extracts produces active viral peptides, including protease 3C, which is responsible for many cleavage reactions [r] ...
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The activity of human rhinovirus 14 3C protease in artificial polyproteins
... The similarity in their genome organisation has already been discussed and this cleavage seen in the reporter polyprotein indicates that the picornavirus genome could encode the structur[r] ...
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Hepatitis A Virus 3C Protease Cleaves NEMO To Impair Induction of Beta Interferon
... were further infected or mock infected with SEV. Luciferase assays were performed 16 h after infection. The results represent the mean and standard deviation from three independent experiments. The firefly luciferase ...
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Coxsackievirus B3 protease 3C induces cell death in eukaryotic cells
... and 3C, are the key proteins that control the virus replication cycle (12, ...of 3C protease is similar to the serine proteinase chymotrypsin; however, 3C protease uses a cysteine ...
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Serological Evidence of Human Klassevirus Infection
... klassevirus 3C protease to demonstrate seroconversion and human infection and to test for seroprevalence in an age-banded pediatric cohort from hospitals in the ...Klassevirus 3C protease ...
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