3D cell culture
Enhanced penetration into 3D cell culture using two and three layered gold nanoparticles
... Human lung adenocarcinoma (A-549) and liver hepatocellular carcinoma (HEPG2) cells were maintained in Dulbecco’s Modi- fied Eagle’s Medium (Sigma Aldrich) with 10% fetal bovine serum and 1% ...
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The 3D Cell Culture System in the Study of Tumor Applications and Prospects
... in 3D, due to their 360˚ surround interac- tion, their internal procedures are initiated to restore their former memories in their native tissue ...a 3D cell culture model, they can ...
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SAHA induced TRAIL sensitisation of Multiple Myeloma cells is enhanced in 3D cell culture
... apoptosis by activation of Caspase-3 and -8. Moreover, TRAIL/HDAC i mediated apoptosis in leukemic cells is associated with cleavage of Bid and Bcl-2, down-regulation of XIAP, moderate reduction on Bcl-XL, and cytosolic ...
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Generating an in vitro 3D cell culture model from zebrafish larvae for heart research
... stem cell technologies, and provide a mechanism for high throughput compound testing and safety pharmacology (Hirt et ...a 3D scaffold-free system (Desroches et ...2D cell monolayers, which lack ...
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Enhanced uptake and transport of PLGA-modified nanoparticles in cervical cancer
... and 3D cell culture, showing that nanoparticles lay- ered with combinations of hexadecanethiol, phosphati- dylcholine and high-density lipoprotein diffused deeper than PEGylated NPs into 3D ...
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Design of synthetic extracellular matrices for probing breast cancer cell growth using robust cyctocompatible nucleophilic thiol yne addition chemistry
... (3D) culture systems are of continued and growing interest for studies of both tissue regeneration and disease, including ...of 3D culture for in vitro studies to recapture in vivo breast ...
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3D Porous Calcium-Alginate Scaffolds Cell Culture System Improved Human Osteoblast Cell Clusters for Cell Therapy
... the cell source for autologous transplantation. Cell pro- liferation is necessary for tissue growth and regenera- tion [33], but long-term culture of primary cells in monolayer might lead to loss of ...
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Collagen Extracted from Persian Gulf Squid Exhibits Anti-Cytotoxic Properties on Apple Pectic Treated Cells: Assessment in an In Vitro Bioassay Model
... a 3D culture system, which closely mimics in vivo ...showed 3D type I collagen system maintained the growth of MDA- MB-231 and MCF-7 breast cancer cells and de- creased the cytotoxicity of apoptotic ...
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The Significance of Cell-related Challenges in the Clinical Application of Tissue Engineering.
... 45 3D cell culture strategies such as self-aggregated spheroids, microcarriers and microencapsulated cells suspended in stirred or spinner vessels provide a potential strategy for stem cells ...
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Cell Dynamics in Three-dimensional (3D) Culture Environments
... transport, cell viability and differentiation within the cell-laden tissue constructs have been ...for 3D cell culture, such as grid networks and hollow hydrogel fibers [159] ...
185
2193-1801-3-80.pdf
... in 3D matrices exhibit altered phenotypes that inhibit their proliferative nature and enhance their ability to form higher order ...mature cell growth in a 3D matrix enhances stem cell ...
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3 Dimensional cell culture: the missing link in drug discovery
... a cell suspension is ...enabling cell–cell ...for cell– matrix ...the cell spheroids ...support 3D cell culture; see [46,47] and review ...
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Combined exposure of diesel exhaust particles and respirable Soufrière Hills volcanic ash causes a (pro-)inflammatory response in an in vitro multicellular epithelial tissue barrier model
... supplemented cell culture medium) to the apical chamber of the insert [20, 29], thus initiating different particle-cell interaction ...these cell types ...immune cell types (macrophages ...
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Enhancement of death receptor mediated apoptosis in multiple myeloma cells
... myeloma cell lines were stained using the optimised PKH26 staining protocol as mentioned above (Section ...using Cell-F software (Olympus) on a dual image of bright field and Texas Red ...
359
Scaffolds for 3D in vitro culture of neural lineage cells
... Acellular tissue engineering scaffolds are typically prepared by removing the cellular components of a tissue or whole organ via chemical, biological or mechanical means, to produce a material that is predominantly ...
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SOX2, a stemness gene, induces progression of NSCLC A549 cells toward anchorage-independent growth and chemoresistance to vinblastine
... with culture medium without ...samples. Cell viability was calculated using the following equation: cell viability (%) = ([LI [uM] × LI [DMSO] ]/100) × 100, where LI [uM] is the average lumines- ...
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Development and characterisation of a 3D multi-cellular in vitro model of normal human breast: a tool for cancer initiation studies.
... functional cell types of breast, detail the phenotype and document our breast cancer initiation ...three cell types were present polarised epithelial structures with lumens and basement membrane production ...
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4-14-2011 12:00 AM A Model System for Rapid Identification and Functional Testing of Genes Involved in Early Breast Cancer Progression
... series 3D model system is of great advantage in this ...parental cell lines stably represent three distinct stages of breast ...epithelial cell line systems, since both show mixed phenotypes and lack ...
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Co-culture of Smooth Muscle Cells and Endothelial Cells on Porous 3D Polyurethane Scaffolds for Vascular Tissue Engineering
... inhibit cell growth. Additionally, the SMCs in co-culture displayed a spindle-like ...two cell layers in culture and found that in the presence of ECs, SMCs had lower proliferative ...two ...
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Human disc cells in monolayer vs 3D culture: cell shape, division and matrix formation
... and 3D culture showed that prote- oglycan expression occurs in monolayer culture but that this microenvironment minimizes the cells production of ...the 3D microenvironment, however, matrix is ...
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