3D culture
Development of a myocardial infarction model by the design of a miniaturized 3D culture platform
... that 3D cell cultures better recapitulate the in vivo microenvironment [29], [65], ...in 3D cell culture as opposed to ...cell culture medium and therefore are able to take nutrients and ...
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A novel MCF-10A line allowing conditional oncogene expression in 3D culture
... Next, we sought to apply the inducible system to study how oncogenes affect the acinar morphogenesis of MCF-10A cells in 3D culture (Figure 3A). We have pre- viously shown that oncogenic B-Raf V600E induces ...
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3D culture models of Alzheimer’s disease: a road map to a “cure-in-a-dish”
... (3D) culture technologies made it possible to generate novel 3D neural cell culture models that recapitulate AD pathologies including robust A β deposition and A β -driven NFT-like tau ...new ...
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Oncogenic activation of FAK drives apoptosis suppression in a 3D-culture model of breast cancer initiation
... in 3D-culture is significantly different from that in ...in 3D-culture, or even mammary gland development in ...in 3D allows it to acquire functions that endogenous FAK does not ...
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Relevance of 3d culture systems to study osteosarcoma environment
... realize 3D culture include naturally derived matrices and synthetic mate- ...for 3D OS culture are alginate, gelatinous protein mixture secreted by Engelbreth-Holm-Swarm (EHS) mouse sarcoma ...
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Matrigel® as 3D culture medium: 2D vs. 3D changes in proliferation and ultrastructure
... of 3D culture become more widely accepted and utilized, many different ECM substitutes are being developed with the potential to mimic cell behavior as it occurs in ...
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Dynamic and influential interaction of cancer cells with normal epithelial cells in 3D culture
... MDCK 3D co-cultures of 8-12 days, many MDA-MB-231 cells were found attached to the surface of MDCK spheres, though not exclusively (Figure 2A, see also Figure ...low-density culture where a small population ...
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Biologic variability of human foreskin fibroblasts in 2D and 3D culture: implications for a wound healing model
... It has not been uncommon to focus on a single strain of cells in experimentation with the 3D collagen matrix. Such practice may decrease variability arising from puta- tive inter-strain diversity; however, if the ...
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Cell Dynamics in Three-dimensional (3D) Culture Environments
... 3D bio-printing has evolved rapidly as a “bottom-up” layer-by-layer manufacturing technique for the construction of complex biological constructs. Functional skin and cartilage, for example, have been fabricated ...
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3D culture of ovarian follicles: a system towards their engineering?
... follicle 3D architecture, with its somatic epithelium surrounding the growing oocyte, has been attempted with a variety of procedures using several type of ma- trices with collagen and alginate being those most ...
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Towards a 3D culture of mouse ovarian follicles
... the culture medium of isobutylmethylxantine (IBMX; to block meiotic resumption) and follicle stimulanting hormone ...The culture protocol applied was capable of maintaining a metabolic coupling with a ...
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A three-dimensional collagen scaffold cell culture system for screening anti-glioma therapeutics
... Then, culture medium was replaced with fresh medium containing various concentrations of the anticancer drugs, temozolomide (TMZ) (Meilun, 85622- 93-1), lomustine (CCNU) (Meilun, 13010-47-4) and cisplatin (DDP) ...
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Development of an in vitro cell-sheet cancer model for chemotherapeutic screening
... vitro 3D culture models that mimic the epithelial tumour microenvironment in vivo are lacking; therefore, we developed a new 3D epithelial cancer model using in vitro cell-sheet engineering to screen ...
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Quantification of Cell Number in 3-Dimensional Cell Culture Construct by Impedance Measurement using Microfluidic Technology
... in 3D cell culture ...a culture chamber embedded with a pair of vertical electrodes located at its opposite ...the 3D culture construct tested was measured via the vertical ...the ...
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The fibroblast Tiam1 osteopontin pathway modulates breast cancer invasion and metastasis
... of 3D spheroid co-cultures containing mammary fibroblasts. Co-culture of human breast cancer cells with Tiam1-deficient fibroblasts induces increased cancer cell invasion into matrix, while ...
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Induction of HOXA9 expression in three-dimensional organotypic culture of the Claudin-low breast cancer cells
... lrECM 3D culture we chose to focus on HOXA9 because of its robust induction in lrECM 3D culture and its critical roles in breast cancer (Figure 1B–1D) ...lrECM 3D culture we ...
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The 3D Cell Culture System in the Study of Tumor Applications and Prospects
... in 3D, due to their 360˚ surround interac- tion, their internal procedures are initiated to restore their former memories in their native tissue ...a 3D cell culture model, they can “intuitively” ...
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Differentiation of Menstrual Blood Derived Stem Cell (MensSCs) to Hepatocyte Liked Cell on Three Dimensional Nanofiberscaffold: Poly Caprolacton (PCL)
... Characterization of MensSCs prior to assessment of the potential of MensSCs to differentiate into hepatocytes was evaluated. High levels of mesenchymal stem cell markers [25] such as CD 44, CD 90, CD 73, and CD 105 and ...
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ErbB2 driven downregulation of the transcription factor Irf6 in breast epithelial cells is required for their 3D growth
... cells (Fig. 6d). As expected, the inhibitor blocked phos- phorylation of the Erk substrate Rsk in all cases (Fig. 6e). Thus, ErbB2/MAPK downregulates Irf6 by suppressing ΔNp63α-dependent signals in cells detached from ...
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2193-1801-3-80.pdf
... of 3D stem cell culture systems, which can include plate or culture dish, spinner flask, rotating wall vessel (RWV), and perfusion bioreactor system among ...or culture dish is the most widely ...
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