3D Cultures
Proton NMR characterization of intact primary and metastatic melanoma cells in 2D & 3D cultures
... the 3D architecture of the multicellular spheroids of the metastatic melanoma cells vary from their monolayer counterpart primarily in the plasma membrane ...the 3D spheroids with the in vivo ...
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Herpes Simplex Virus 1 Infection Promotes the Growth of a Subpopulation of Tumor Cells in Three-Dimensional Uveal Melanoma Cultures
... in 3D are more resistant to HSV-1 infection than cells grown in 2D (monolayer) cultures and that HSV-1 may establish a quiescent infection in some melanoma cells in 3D cultures (12, 13, ...of ...
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Foot-and-Mouth Disease Virus Replicates Only Transiently in Well-Differentiated Porcine Nasal Epithelial Cells
... in 3D cultures, the presence of a few floating dead cells could be ...of 3D nasal mucosal epithelial (NME) ...FMDV-infected 3D NME cultures confirms the increase of IFN-  mRNA ...
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The relevance of using 3D cell cultures, in addition to 2D monolayer cultures, when evaluating breast cancer drug sensitivity and resistance
... in 3D cells in comparison to 2D cells, while results for pHER2 were ...in 3D cells mediates part of the resistance of the cells cultured in 3D to the effects of neratinib and ...in 3D compared ...
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Innovative approaches to establish and characterize primary cultures: an ex vivo 3D system and the zebrafish model
... primary cultures are often limited by the small amount of material available, while conventional in vitro systems might alter the features and behavior that characterize cancer ...a 3D scaffold-based system ...
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Chondrogenic differentiation potential of osteoarthritic chondrocytes and their possible use in matrix associated autologous chondrocyte transplantation
... scaffold cultures was subjected to gene expression analysis using oligonucleotide microarray HG- ...ML cultures as baseline, for OA and ND separately. Furthermore, OA ML and 3D cultures were ...
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Development of a 3D Tissue Culture-Based High-Content Screening Platform That Uses Phenotypic Profiling to Discriminate Selective Inhibitors of Receptor Tyrosine Kinases.
... In 3D culture, activation of c-Met can result in invasion of tumor cells into the surrounding microenvironment, 15 a pro- cess that closely resembles the in vivo ...of 3D cultures of invasive ...
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A novel MCF-10A line allowing conditional oncogene expression in 3D culture
... for 3D culture experiments as described previously ...of 3D cultures with the antibodies specified below was performed as described previously ...
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Cell Counting and Viability Assessment of 2D and 3D Cell Cultures: Expected Reliability of the Trypan Blue Assay
... Background: Whatever the target of an experiment in cell biology, cell counting and viability assessment are always computed. The Trypan Blue (TB) assay was proposed about a century ago and is still the most widely used ...
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MR Imaging Features of High Grade Gliomas in Murine Models: How They Compare with Human Disease, Reflect Tumor Biology, and Play a Role in Preclinical Trials
... the phenotypic traits of the native tumor, and displays more cellular heterogeneity than established cell lines.6 The use of spheroids 3D cultures, as opposed to cell suspensions 2D cult[r] ...
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Comparison of NSAIDs activity in COX-2 expressing and non-expressing 2D and 3D pancreatic cancer cell cultures
... Methods: To test the effect of compounds on the viability of cells, the MTT assay was used. The activity of NSAIDs in 3D cell cultures was evaluated by measuring the size change of spheroids. The type of ...
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A 3-dimensional in vitro model of epithelioid granulomas induced by high aspect ratio nanomaterials
... and 3D cultures in order to minimize acute toxicity and to reflect realistic occupational exposures in humans and in previous inhalation studies in ...this 3D in vitro ...
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γ Secretase inhibition promotes cell death, Noxa upregulation, and sensitization to BH3 mimetic ABT 737 in human breast cancer cells
... or 3D cultures of breast cancer cells plus a novel preclinical short-term ex vivo assay that correctly maintains human mammary tissue integrity and preserves tumor microenvironment, we tested the effects of ...
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PubMedCentral-PMC4850325.pdf
... to 3D cultures, al- lowing for cells to continue to grow and interact with their mechanical environment with no evidence of disturbance by the nanoprobes; PEGylated GNRs have been shown to lack cellular ...
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Disulfide-Based Diblock Copolymer Worm Gels: A Wholly-Synthetic Thermoreversible 3D Matrix for Sheet-Based Cultures
... supported 3D cell culture provides a convenient means of handling and analysing 3D cell cultures, while thermo responsive hydrogels provide a convenient vehicle to deliver and embed cells into the ...
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High-throughput confocal imaging of differentiated 3D liver-like spheroid cellular stress response reporters for identification of drug-induced liver injury liability
... differentiated 3D liver-like spheroid ...into 3D spheroids similar to wild-type HepG2 ...six 3D GFP reporters in single 48 h treatment or 6 day daily repeated ...in 3D sphe- ...differentiated ...
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Benchtop technologies for circulating tumor cells separation based on biophysical properties
... In order to convey the proper control of DEP force on blood sample, it is necessary for researchers to select or develop appropriate microelectrode designs which fix their studies requirements. For the existing DEP ...
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Consistent and reproducible cultures of large scale 3D mammary epithelial structures using an accessible bioprinting platform
... organoid cultures of mammary epithelial cells inside col- lagen matrices were introduced over four decades ago ...In 3D culture, multiple parameters operate together to affect both experimental outcomes and ...
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Three-Dimensional Structure of Aleutian Mink Disease Parvovirus: Implications for Disease Pathogenicity
... The Sf9-infected cultures were harvested 72 h The 3D reconstruction of ADVG-VP2, computed to 22 Å resolution from images of vitrified particles, reveals a capsid structure with mean exte[r] ...
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β1 Integrin/FAK/cortactin signaling is essential for human head and neck cancer resistance to radiotherapy
... In line with a lack of FAK phosphorylation, we found reduced phosphorylation of FAK-associated proteins, paxillin, p130Cas, and JNK1, in both 3D lrECM UTSCC15 and XF354 cell cultures and[r] ...
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