3P(2) line
Original Article Effect of microRNA338-3p on proliferation of hepatoma cells and its mechanisms
... cell line HepG2, hepatic cancer tissue, adjacent cancer tissue and normal hepatic tissue, fol- lowed by reverse transcription into cDNA with the use of TaqMan MicroRNA reverse tran- scription ...Mix, 2 uL ...
7
Pionic Deuterium
... Figure 2 shows such a prediction for πH where energies have been scaled to the πD ...1s) line shape that the first ESCM predictions could not describe the measured spectrum ...measured line shape; a ...
10
14q32-encoded microRNAs mediate an oligometastatic phenotype
... cell line co-labeled with green fluorescent protein (GFP) and luciferase [23] and transfected with miR mimics to evaluate the influence of each miR on subsequent metastasis ...At 2 weeks following ...
13
Effect of miR-27a-3p on Proliferation and Migration in Non-small Cell Lung Cancer Cells
... Lung cancer was the most common malignancy throughout the world. For the lack of effective diagnostic methods at the early stages of lung cancer, most patients were diagnosed at an advanced stage. Accordingly, it was ...
8
MicroRNA-214-3p inhibits proliferation and cell cycle progression by targeting MELK in hepatocellular carcinoma and correlates cancer prognosis
... Downregulation of miR‑214‑3p in primary HCC tissues We first determined miR-214-3p expression in HCC cell lines (HepG2, HUH-7 and SNU398) and a nor- mal hepatic cell line (L-O2). Our results showed ...
9
miR-27b-3p suppresses cell proliferation through targeting receptor tyrosine kinase like orphan receptor 1 in gastric cancer
... The expression level of ROR1 was analysed in 56 paired human GC specimens by using real-time PCR. It was shown that the upregulation of ROR1 was significant in the GC tissues compared to the paired adjacent non- tumor ...
13
<p>Effect of LINC00657 on Apoptosis of Breast Cancer Cells by Regulating miR-590-3p</p>
... At present, many literatures have suggested that lncRNA can be used as ceRNA to regulate RNA transcripts through competition with miR (microRNA) and promote changes in biological functions of various tumor cells. 26,27 ...
11
Original Article MicroRNA-299-3p promotes the sensibility of lung cancer to doxorubicin through directly targeting ABCE1
... In this study, we test the hypothesis that miR- NA-299-3p promotes the sensibility of lung cancer to doxorubicin through directly targeting adenosine triphosphate (ATP) binding cassette E1 (ABCE1). First, ...
10
miR-142-3p reduces the size, migration and contractility of endometrial and endometriotic stromal cells by targeting integrin- and Rho GTPase- related pathways that regulate cytoskeletal function
... The St-T1b cell line [24] and primary endometriotic ectopic lesion-derived stromal cells (ESCs) were maintained in 70% DMEM/ 18% MCDB 105 media (Sigma-Aldrich, cat. No. 117-500) supplemented with 10% FBS, 1% ...
15
RACK1 promotes miR-302b/c/d-3p expression and inhibits CCNO expression to induce cell apoptosis in cervical squamous cell carcinoma
... © The Author(s) 2020. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you ...
12
mir-101-3p is a key regulator of tumor metabolism in triple negative breast cancer targeting AMPK
... cell line MCF- 10A, human breast cancer cell lines MDA-MB-231, MDA-MB-435, MDA-MB-468, MCF-7, T47D, BT-474, BT-483, and SKBR3 were obtained from the American Type Culture Collection (Manassas, VA, USA) and were ...
11
<p>HIF1α/miR-520a-3p/AKT1/mTOR Feedback Promotes The Proliferation And Glycolysis Of Gastric Cancer Cells</p>
... in the GC tissues provided by patients with stage III – IV disease (TNM staging) than in those provided by patients with stage I – II disease (P<0.01) (Figure 1B). Next, we detected the expression level of ...
12
Splicing-dependent expression of microRNAs of mirtron origin in human digestive and excretory system cancer cells
... cell line transfected with native (WT) or splicing- deficient (MUT) minigenes was analyzed using reverse transcription-quantitative polymerase chain reaction ...
11
Original Article MicroRNA-129-3p functions as a tumor suppressor in serous ovarian cancer by targeting BZW1
... cell line, SKOV3 cells. The results demonstrated that miR-129-3p overexpression distinctively inhibited the proliferation, migra- tion, and invasion of ovarian cancer ...
8
<p>CircRNA SMARCC1 Sponges MiR-140-3p to Regulate Cell Progression in Colorectal Cancer</p>
... cell line (NCM460) was detected using ...cell line. The effects of circSAMRCC1 and miR- 140-3p on cell proliferation were investigated using CCK8 and colony formation assays, respec- ...
12
Original Article miR-24-3p suppresses cellar proliferation in hepatocellular carcinoma
... We first verified the expression level of miR-24- 3p in twenty paired of HCC and adjacent tis- sues, and 10 normal tissues by RT-PCR. As shown in Figure 1A, we found that tumor tis- sues showed aberrant down ...
9
Operationalising the Lean principles in maternity service design using 3P methodology
... (or 3P), the Lean principles of understanding user value, mapping value-streams, creating flow, developing pull processes and continuous improvement were applied to the design of a new maternity ...in line ...
8
Are circulating microRNAs suitable for the early detection of malignant mesothelioma? Results from a nested case–control study
... Malignant mesothelioma is an asbestos-related malig- nancy with increasing incidence worldwide [1]. Based on the continued use of asbestos and a long latency period of up to 50 years mesothelioma remains a global health ...
6
MicroRNA-338-3p suppresses ovarian cancer cells growth and metastasis: implication of Wnt/catenin beta and MEK/ERK signaling pathways
... RNAs (piRNAs) [34]. MiRNAs could play crucial roles as oncogene or antioncogene in human cancers. MiR- NAs regulated cancer related growth, metabolism, gen- omic stability, apoptosis, epithelial to mesenchymal transition ...
13
<p>MiR-27a-3p Targeting GSK3β Promotes Triple-Negative Breast Cancer Proliferation and Migration Through Wnt/β-Catenin Pathway</p>
... worldwide. 2 Triple-negative breast cancer (TNBC) accounts for 15% to 20% of newly diagnosed breast cancer cases and is known for its aggressive biological behavior and poor patient outcomes compared with hormone ...
9