3T3-L1 preadipocyte
Critical role of leukotriene B4 receptor signaling in mouse 3T3-L1 preadipocyte differentiation
... mouse 3T3-L1 preadipocyte ...mouse 3T3-L1 preadipocytes. Mouse 3T3-L1 preadipocytes were treated with NDGA, AA-861 or ONO-4057 for 6 ...
7
Anti adipogenic and anti obesity activities of purpurin in 3T3 L1 preadipocyte cells and in mice fed a high fat diet
... Methods: Our study will test the effect purpurin has on adipogenesis using both in vitro and in vivo models. The in vitro model consists of tracking with various biomarkers, the differentiation of pre-adipocyte to ...
12
Glycoprotein Attributed Effect of Flatfish Extract on 3T3-L1 Preadipocyte Differentiation
... The biological activities of water-soluble extracts obtained from the flatfish (Pleuronectes obscurus) tail fin which is a waste material generated by the fish processing industry were investigated in this study. The ...
10
Twist 1 regulates the expression of PPARγ during hormone-induced 3T3-L1 preadipocyte differentiation: a possible role in obesity and associated diseases
... ACTCCAAGATGGCAAGCTG-3’. Packaged viruses en- coding an shRNA targeting Twist 1 based on the obtained plasmids (pLV3/Twist 1-shRNA) were applied to cultured 293T cells according to the manufacturer’s instructions. The ...
11
EVI1 Mediated Stimulation of 3T3 L1 Preadipocyte Differentiation Is CtBP Dependent
... in 3T3-L1 ...of 3T3-L1 cells (Materials and Methods). The 3T3-L1 cells were re-infected with virus con- taining supernatant again 24 hrs ...
15
Trapa japonica Flerov Extract Attenuates Lipid Accumulation through Downregulation of Adipogenic Transcription Factors in 3T3 L1 Cells
... inhibited 3T3-L1 adipogenesis and fat accumulation (Figure ...inhibits 3T3-L1 adipogenesis at a relatively early stage by regulating the expression of PPARγ and C/EBPα (Figure 3(a) & ...
10
MiR27a Promotes the Development of Macrophage-like Characteristics in 3T3-L1 Preadipocytes
... in 3T3-L1 preadipocyte cells, phagocytosis of neutral red or fluorescent microspheres were determined in cells transfected with or without miR27a ...
11
1α, 25 dihydroxy Vitamin D3 containing fractions of Catharanthus roseus leaf aqueous extract inhibit preadipocyte differentiation and induce lipolysis in 3T3 L1 cells
... Our quest for an anti-obesity therapeutic agent, lead us to investigate the medicinal herb C. roseus, which had well acclaimed and documented activity against cancer, diabetes and liver diseases. This study showed for ...
17
Original Article MicroRNA-142a-3p promotes the differentiation of 3T3-L1 preadipocytes by targeting high-mobility group AT-hook 1
... during 3T3-L1 preadipocyte ...promoted 3T3-L1 preadipocyte ...in 3T3-L1 preadipocytes. Moreover, the knockdown of HMGA1 induced 3T3-L1 ...
8
INHIBITORY EFFECT OF ETHANOLIC EXTRACT OF CITRUS PARADISI AND NARINGIN ON LIPID ACCUMULATION IN 3T3 L1 CELL LINES
... of 3T3-L1 preadipocyte. 3T3-L1 preadipocytes were seeded at a density of 3×10 6 cells/ well into 96-well plates and maintained with Dubelcco‘s modified eagle’s medium(DMEM) containing ...
15
Development of a Novel Anti-Obesity Compound with Inhibiting Properties on the Lipid Accumulation in 3T3-L1 Adipocytes
... So far, the effectiveness of hundreds of herbs and active ingredients has been investigated for the obesity and metabolic syndrome treatment. For instance, the active ingredient of berberine, found in the plants of ...
9
Activities of jamu and spices on adipocytes formation
... Figure 1. Effects of cinnamon extract on adipogenesis of 3T3-L1 preadipocytes. Differentiation is induced by addition inducers as indicated in Table I. After 11 days, preadipocytes differentiation was ...
7
mVps45 knockdown selectively modulates VAMP expression in 3T3-L1 adipocytes
... I nsulin stimulates the delivery of glucose transporter-4 (GLUT4)-containing vesicles to the surface of adipocytes. Deple- tion of the Sec1/Munc18 protein mVps45 significantly abrogates insulin-stimulated glucose ...
5
An Herbal Formula LI85008F Inhibits Lipogenesis in 3T3 L1 Adipocytes
... 3T3-L1 adipogenic differentiation requires synergistic ac- tivity of multiple transcription factors and adipogenic mo- dulatory factors including PPARγ, C/EBP, aP2 etc. [16]. Among them, PPARγ is the key ...
9
Curcumin Inhibits Adipogenesis Elicited by Clozapine in 3T3 L1 Cells
... Compared to exposure to DM alone, exposure to both 20 µM CZP and DM resulted in a significant increase in TG levels (1.3 fold in Figure 1(b)), Oil Red O-stained cells (Figure 2(a)), and percentage of lipid-filled ...
11
ANTIHYPERGLYCEMIC ACTIVITY OF TINOCRISPOSIDE BY STIMULATING 3T3 L1 ADIPOCYTE CELL DIFFERENTIATION
... In a previous study, we have investigated the effect of tinocrisposide in lowering of blood glucose level on alloxan-induced hyperglycemic mice [5]. Adipocyte cells have a function as a blood glucose level regulation, ...
5
Materials Discovery by Crystal Growth: Synthesis, Structure Determination, Magnetic, and Optical Properties of Complex Lanthanide Containing Oxides, Oxyhydroxides, and Oxyfluorides
... in 3T3-L1 cells (Chapter 2), we also wanted to prepare 3T3-L1 adipocytes with fumarase shRNA knockdown (Fh k/d) to better understand the role of autophagy in succinated protein turnover within ...
72
Action of nicotinic acid on the reversion of hypoxic-inflammatory link on 3T3-L1 adipocytes
... Analysis of HIF-1 ɑ , PPAR α and PPAR γ by western blot Total protein was extracted from 3T3-L1 adipocytes. Next, the samples were concentrated using Centrifugal Filter Devices (Milipore, St. Charles, MO, ...
8
IN VITRO ANTI OBESITY EFFICACY OF SELECTED PLANTS IN 3T3 L1 CELL LINE
... ABSTRACT: Background: Obesity is a confronting global issue with excess accumulation of body fat to an extent that decreases life expectancy. It is multifactorial in origin; genetic, environmental and pathophysiological. ...
8
Lipolytic Effect of Eumicrotremus orbis Water Extracts in Differentiated 3T3-L1 Adipocytes
... differentiatied 3T3-L1 adipocytes and its ...differentiated 3T3-L1 adipocytes treated EOWE, hormone sensitive lipase (HSL) and phosphorylated HSL (p-HSL) protein level increased by ...
6