40 T
Interaction of Simian Virus 40 chromatin with Simian Virus 40 T-antigen.
... However, when chromatin was isolated from the variant 1103 19, which contains nine copies of the origin of DNA replication and lacks at least one and possibly two of the other high-affin[r] ...
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ATP-Dependent Simian Virus 40 T-Antigen–Hsc70 Complex Formation
... virus 40 large T antigen is a multifunctional oncoprotein that is required for numerous viral functions and the induction of cellular ...transformation. T antigen contains a J domain that is required ...
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Association of Simian Virus 40 T Antigen with Simian Virus 40 Nucleoprotein Complexes
... To determine the functional significance of the SV40 lOOK T antigen in SV40 nucleoprotein complexes containing SV40 I DNA, cells were infected with SV40 tsA58, a temperature-sensitive mu[r] ...
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Intestinal Dysplasia Induced by Simian Virus 40 T Antigen Is Independent of p53
... and T antigen-mouse monoclonal PAb419 (25); p53-rabbit poly- clonal FL-393 (sc-6243), p53-mouse monoclonal PAb240 (sc-99), MDM2-mouse monoclonal SMP14 (sc-965), p21-rabbit polyclonal M-19 (sc-471), and vimentin- ...
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Inhibition of simian virus 40 T-antigen expression by cellular differentiation.
... Although none of the other clones differentiated as well as CSV3-1, repression of T-antigen expression was observed in all these cell clones as they underwent adipocyte differentiation, [r] ...
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Mechanisms of simian virus 40 T-antigen activation by phosphorylation of threonine 124.
... for T-antigen binding and indicate relative binding affin- ...wild-type T antigen (lanes W) with the binding of mutant T antigen with an alanine substitution for Thr-124 (lanes ...in T ...
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trans-dominant defective mutants of simian virus 40 T antigen.
... As a further demonstration of the difference between complementing and noncomplementing T antigen mutants, we constructed BSC40 cell lines that produce mutant T antigens and used these l[r] ...
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Dimerization of simian virus 40 T-antigen hexamers activates T-antigen DNA helicase activity.
... comparing T-antigen binding to the DNA fork and DNA bubble, reaction mixtures containing ...of T antigen, ...examining T antigen-DNA complex formation or the oligomeric state of T antigen, ...
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Simian virus 40 T antigen as a carrier for the expression of cytotoxic T-lymphocyte recognition epitopes.
... The results show that the translocated SV40 T antigen site I and the HSV gB CTL epitopes in the chimeric T antigens, when expressed in immortalized C57BL/6 B6 mouse kidney cells, were ef[r] ...
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Cell-free synthesis of simian virus 40 T-antigens.
... In addition to the data presented here, strong evidence in support of the model for the location of sequences coding for the SV40 T-Ags has recently come from experiments which have show[r] ...
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Activation of simian virus 40 transcription in vitro by T antigen.
... 4591 Downloaded from http://jvi.asm.org/ on November 9, 2019 by guest Simian virus 40 is repressed when the viral early gene product large tumor antigen TAg binds to specific sites withi[r] ...
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Salt-resistant association of simian virus 40 T antigen with simian virus 40 DNA in nucleoprotein complexes.
... Sucrose gradi- Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest I.l ent-purified SV40 NPCs, labeled with [35S]methionine and [3H]thymidine, were treated with increasing [r] ...
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Association of simian virus 40 T antigen with replicating nucleoprotein complexes of simian virus 40.
... Recently, Mann and Hunter 30 detected SV40 large T antigen in the fraction of a sucrose gradient containing mature 70S NPCs by immunoprecipitation of T antigen labeled with [3S]methionin[r] ...
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Measurements of the molecular size of the simian virus 40 large T antigen.
... This would be 218 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest A measure of the molecular weight of the large simian virus 40 T antigen was sought by SDS-polyacrylam[r] ...
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Monoclonal antibody to simian virus 40 small t.
... It is clear that PAb280 efficiently immunoprecipitated small t but not large T from extracts of wild-type SV40-infected cells even though large T was present in the cell extract in a for[r] ...
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Simian Virus 40-Induced T and Tumor Antigens
... The major component of the SV40 tumor antigen extracted from transplanted hamster tumors was identified at a position in centrifuged linear sucrose gradients corresponding to a molecular[r] ...
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Mutational analysis of simian virus 40 small-t antigen.
... 2895 Downloaded from http://jvi.asm.org/ on November 10, 2019 by guest Several point mutations in the simian virus 40 SV40 small-t antigen have been analyzed for their effects on protein[r] ...
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Structure and synthesis of a simian virus 40 super T-antigen.
... DISCUSSION In productively infected permissive cells, the early region of SV40 DNA is transcribed into two mRNAs of 2.6 and 2.3 kb, which encode proteins of apparent molecular weights 17[r] ...
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Functional analysis of a simian virus 40 super T-antigen.
... We have not compared the properties of the super and large T-antigens in the maintenance of transformation, but noted that cells transformed by the super T-antigen templates and the C120[r] ...
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Induction of p53-Independent Apoptosis by Simian Virus 40 Small t Antigen
... Simian virus 40 small t antigen (st) is required for optimal transformation and replication properties of the virus. We find that in certain cell types, such as the human osteosarcoma cell line U2OS, st is ...
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