5′AMP-activated kinase
Metformin induces apoptotic cytotoxicity depending on AMPK/PKA/GSK-3β-mediated c-FLIP<sub>L</sub> degradation in non-small cell lung cancer
... significantly activated Adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK) and its downstream glycogen synthase kinase 3beta (GSK-3β), block the expression of AMPK, ...
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Molecular Mechanisms and Insights into the Nampt Inhibitor (FK866) Resistance in Cancers
... Nicotinamide phosphoribosyltransferase (NAMPT), the rate-limiting enzyme in NAD+ biosynthesis from nicotinamide, is one of the major factors regulating cell metabolism and NAMPT is considered a promising target for ...
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The regulation of heart AMP-activated protein kinase by long-chain fatty acids and hormones
... casein kinase II, AMPK, PKC or protein phosphatases 2A and 2C produced no change in enzyme ...AMPK, 5- aminoimidazole-4-carboxamide-1-p-D-ribofuranoside (AlCAR), an effect reversed by protein phosphatase 2A ...
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AMP-activated protein kinase-α1 as an activating kinase of TGF-β-activated kinase 1 has a key role in inflammatory signals
... the kinase activity of TAK1 or AMPK-a1 induced by LPS stimulation was also significantly reduced in AMPK-a1 KD or TAK1 KD THP-1 cells, respectively, as compared with that of wt THP-1 cells (Figure 2g, TAK1; Figure ...
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AMP activated protein kinase: a potential therapeutic target for triple negative breast cancer
... TNBC treatments consist of two parts, namely locoregional treatments, including surgery and radiotherapy, and sys- temic treatments, such as chemotherapy and targeted ther- apy. Compared with locoregional treatments, ...
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A possible role for AMP-activated protein kinase activated by metformin and AICAR in human granulosa cells
... containing 5% skim milk (Difco, Detroit, MI) for 1 h at room temperature and washed with three changes of Tris-buffered saline (TBS; 20 mM Tris, 137 mM NaCl, pH ...phospho-p70S6 kinase, rabbit poly- clonal ...
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Mesoglycan attenuates VSMC proliferation through activation of AMP-activated protein kinase and mTOR
... Whole cell extracts were prepared by lysing the cells in pro-prep protein extract buffer. The protein concentration was quantified with protein assay reagent from Bio-Rad (Hercules, CA, U.S.A.). Equal amounts of protein ...
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AMP-Activated Protein Kinase and Glycogen Synthase Kinase 3β Modulate the Severity of Sepsis-induced Lung injury
... Bone marrow neutrophils were purified using a negative selection column (31,38). In brief, bone marrow cell suspensions were isolated from the femur and tibia of mice by flushing with RPMI 1640 medium with 5% ...
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AMP-activated kinase (AMPK) regulates activity of HER2 and EGFR in breast cancer
... Sigma-Aldrich. 5-Amino-4-imidazole carboxamide riboside (AICAR), a pharmacological activator of AMPK (Toronto Research Chemicals, Toronto, ON) was dissolved in DMSO to make a 500mM stock ...
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Role of AMP activated protein kinase in mechanism of metformin action
... for 20 hours and then re-fed for 2 hours before the final dose. Four hours after the final dose, tissue and blood samples were obtained for analysis (see Methods). During star- vation, there should be very little lipid ...
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LKB1 and AMPK and the cancer-metabolism link - ten years after
... upstream kinase acting on AMP-activated protein kinase (AMPK) as a complex containing LKB1, a known tumor ...protein kinase, AMPK, which at the time was mainly associated with ...
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Augmenting energy expenditure by mitochondrial uncoupling: a role of AMP-activated protein kinase
... serine/threonine kinase, which is activated by any metabolic stress, often in response to increased AMP/ATP ratio (Hardie 2008; Zhang et ...of AMP to the c subunit results in an allosteric ...
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Baicalin ameliorates polycystic ovary syndrome through AMP-activated protein kinase
... anovulatory infertility. PCOS is characterized by reproductive dysfunction and metabolic disorders. Baicalin (BAL) is one of the most potent bioactive flavonoids isolated from the radix of Scutellaria baicalensis. In the ...
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Identification of a nuclear export signal in the catalytic subunit of AMP-activated protein kinase
... Feeding Assays. To assess food intake, we employed the CAFE assay (Ja et al., 2007), which quantifies dietary intake using a volumetric capillary feeder. We placed three 3-5 day old adult flies in the CAFE chamber ...
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AMP-activated protein kinase regulates L-arginine mediated cellular responses
... methylsulfonyl fluoride). The cell lysates were then son- icated twice for 10 s and then centrifuged at 14,000 × g for 20 min at 4°C. The pellets were discarded and super- natants were assayed for protein concentration ...
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AMP-activated protein kinase is required for the anti-adipogenic effects of alpha-linolenic acid
... [14,15]. In addition, n-3 PUFA also has many effects in- cluding stimulation of 5′AMP-activated protein kinase (AMPK) in adipose tissue [16]. AMPK is a heterotri- meric enzyme that plays key ...
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Suppression of Kaposi's Sarcoma-Associated Herpesvirus Infection and Replication by 5′-AMP-Activated Protein Kinase
... result, activated AMPK restricts infection by several RNA viruses by decreasing levels of fatty acid synthesis ...of activated AMPK (16, ...endogenous activated AMPK can dramat- ically enhance KSHV ...
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Protein kinase C phosphorylates AMP-activated protein kinase α1 Ser487
... Ser487 kinase, the synthetic PKC activator PMA rapidly stimulated phosphorylation of both the PKC substrate MARCKS and AMPKα1 Ser487 (Figure ...± 5% (Figure ...
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AMP-Activated Protein Kinase Is Required for the Macropinocytic Internalization of Ebolavirus
... Compound C was evaluated for inhibition of replication-com- petent EBOV that expresses EGFP during infection (Fig. 3). Infec- tions were performed for 24 h in the presence of serial dilutions of compound C. The numbers ...
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Mitochondrial content, oxidative, and nitrosative stress in human full-term placentas with gestational diabetes mellitus
... SDS-PAGE and western blot were performed as previ- ously described [12]: 30 μg of placenta lysate was sepa- rated on 10% polyacrylamide gel, and resolved proteins were transferred to nitrocellulose membrane. Molecular ...
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