5-aza-2’-dC
A high-throughput screen of inactive X chromosome reactivation identifies the enhancement of DNA demethylation by 5-aza-2′-dC upon inhibition of ribonucleotide reductase
... and 5-aza-2 ′ -dC on myeloid leukemia cell line proliferation, we applied a luminescence-based cell viability assay that linearly scales with cell number and titered HU and ...
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The Mbd1 - Atf7ip - Setdb1 pathway contributes to the maintenance of X chromosome inactivation
... agent 5-aza-2’-dC (Figure 1Di, Additional file 3: Figure ...of 5-aza-2’-dC (10 uM) (Additional file 4: Figure S4B, ...
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Generation of tumor initiating cells by exogenous delivery of OCT4transcription factor
... three-dimensional; 5-aza-2 ’ dC: 5-aza-2 ’ -deoxycytidine; CDH1: E-cadherin; CK: cytokeratin; CTG: Cell Titer Glo; DMEM: Dulbecco ’ s modified Eagle ’ s medium; EMT: ...
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A subgroup of pancreatic adenocarcinoma is sensitive to the 5-aza-dC DNA methyltransferase inhibitor
... of 5-aza-dC on cell coming from this tumor ...the 5-aza-dC has shown an objective response in some patients, its overall efficiency remains relatively ...reason ...
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Effects of 5 aza 2’ deoxyctidine on the development of porcine parthenogenetic and nuclear transfer embryos
... to 5 nM 5-aza- dC for 1 hour promotes further development by trigger- ing adjustment of methylation status without injuring ...with 5 nM 5-aza-dC for 1 to 20 hours ...
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The Effects of 5-Aza-2´-deoxycytidine on Total Lipid and Fatty Acid Composition of Apanteles galleriae Wilkinson (Hymenoptera: Braconidae) and on Its Parasitized Host
... acids, 5-Aza-dC did not cause significant changes in the percent of different fatty acid classes for all ...that 5-Aza-dC causing irreversible adverse effect on insects examined ...
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Original Article Age-related methylation of genomic DNA in human adipose-derived stem cells
... with 5-Aza-dC, both OCT-4 and NANOG expression levels were significantly increa- sed, further confirming that both OCT-4 and NANOG expression can be restored by treat- ment with a demethylating drug, ...
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Downregulation of TES by hypermethylation in glioblastoma reduces cell apoptosis and predicts poor clinical outcome
... with 5-aza-dC can greatly increase TES expression, confirming the hypermethylation of TES promoter in ...with 5-aza-dC can highly release TES expression resulting in significant ...
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Aberrant methylation-mediated downregulation of lncRNA SSTR5-AS1 promotes progression and metastasis of laryngeal squamous cell carcinoma
... before 5-Aza-dC treatment and the methylation status was com- pletely reversed after 5-Aza-dC treatment, suggesting the important role of exon 1 methylation of SSTR5 and pro- ...
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DNA methylation suppresses chitin degradation and promotes the wing development by inhibiting Bmara-mediated chitinase expression in the silkworm, Bombyx mori
... to 5-aza-dC treatment, luciferase activ- ity assays in Bm12 cells were ...the 5-aza-dC treatment, especially the − 300 to − 200 nt fragment ...that 5-aza-dC ...
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Gene Expression Profiling of Human Myeloid Leukemic MV4 11 Cells Treated with 5 Aza 2’ deoxycytidine
... analog, 5-aza-2’-deoxycytidine (5-aza-dC) is a DNA methyltransferase inhibitor that triggers DNA de- methylation leading to the reactivation of epigenetically silenced tumor ...
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Overexpression of FES might inhibit cell proliferation, migration, and invasion of osteosarcoma cells
... after 5-Aza- 2 ′ -deoxycytidine (5-Aza-dC) treatment were selected for further functional study, including proliferation, cell cycle, migration, and invasion ...
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DNA methylation of the RIZ1 tumor suppressor gene plays an important role in the tumorigenesis of cervical cancer
... Silencing of tumor suppressor genes is important in initiation and progression of cancers. RIZ1 has been reported to possess tumor suppressor activity, and its expression is lost in many carcinoma cell lines and pri- ...
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Circulating cell free DNA based epigenetic assay can detect early breast cancer
... -Aza-dC: 5 ’ -Aza-2-deoxycytidine; AUC: area under the curve; BC: breast cancer; BORN: Breast Oncology Research Network; cfDNA: circulating cell-free DNA; CNA: copy number alteration; ...
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Epigenetic inactivation of HOXD10 is associated with human colon cancer via inhibiting the RHOC/AKT/MAPK signaling pathway
... Methods: Thirty-seven paired colon cancer and its adjacent samples from The Cancer Genome Atlas (TCGA) were analyzed. Chip Analysis Methylation Pipeline (ChAMP) analysis was employed for differential methylated points ...
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Epigenetic silencing of TGFBI confers resistance to trastuzumab in human breast cancer
... file 2: Figure ...agent 5-aza-2 ′ -deoxycytidine (5-aza-dC) in the AUTR methylated cell line by ...after 5-aza-dC treatment by Western ...
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Low-Dose 5-Aza-2′-deoxycytidine Pretreatment Inhibits Experimental Autoimmune Encephalomyelitis by Induction of Regulatory T Cells
... 10-d 5-Aza could prevent autoimmune disease in the CNS of a murine EAE model (Figure ...short-term 5-Aza-pretreated MOG- induced mice developed EAE during our observation period (Figure ...
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Methylation status of insulin-like growth factor-binding protein 7 concurs with the malignance of oral tongue cancer
... suggesting the presence of MET in IGFBP-7 overex- pressed A253-5 cells. Additionally, the inactivation of AKT is also noted in IGFBP-7 overexpressed A253-5 cells. In general, AKT modulates numerous ...
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The role of cigarette smoke-induced epigenetic alterations in inflammation
... melanoma 2 (AIM2) and involved in inflammasome response, was associated with lower expression of AIM2 and lower CRP ...A 2-step epigenetic Mendelian rand- omization study conducted by Jhun et ...
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Insulin like growth factor binding protein 7 (IGFBP7) expression is linked to poor prognosis but may protect from bone disease in multiple myeloma
... Results: Median IGFBP7 expression is significantly lower in CD138-purified plasma cells from individuals with MGUS and MM, compared to normal bone marrow plasma cells. IGFBP7 gene expression in MM cells is regulated by ...
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