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Expression and characterization of an RNA capping enzyme encoded by Chlorella virus PBCV-1.

Expression and characterization of an RNA capping enzyme encoded by Chlorella virus PBCV-1.

... (50 mM Tris HCl [pH ...to 50 mM NaCl by the addition of 2 volumes of buffer A and then applied to a 35-ml column of DEAE-cellulose that had been equilibrated with buffer A containing 50 ...

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The splashing morphology of liquid-liquid impacts

The splashing morphology of liquid-liquid impacts

... 3 mm thick with overall inner dimensions of 200 × 200 × 100 ...× 50 mm (Figure ...approximately 50 times greater than the drop diameter thereby eliminating any effects associated with the ...

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Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes

Heterologous expression of Pycnoporus cinnabarinus cellobiose dehydrogenase in Pichia pastoris and involvement in saccharification processes

... in 50 mM sodium acetate buffer (pH 5) with 4 mM sodium fluoride for CDH and 50 mM sodium tartrate buffer (pH 4) for ...adding 5 mM ABTS to stain for laccase and ...

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Analysis of milling of dry compacted ribbons by distinct element method

Analysis of milling of dry compacted ribbons by distinct element method

... 20 mm diameter rotate around the horizontal axis of the mill, with their axes at a radial distance from the mill axis to provide zero clearance with the base of the mill, as shown in ...1.8 mm diameter ...

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Factors Affecting the Efficiency of Fibers in Concrete on Crack Reduction

Factors Affecting the Efficiency of Fibers in Concrete on Crack Reduction

... 48 mm length, hooked-end steel fibers of diameters 0.6 mm and 1.05 mm, both of 50 mm ...samples, 5 rings were ...of 5 kg/m 3 and higher, did reduce crack widths on par ...

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Soil crumbling during tillage as a function of soil organic matter content

Soil crumbling during tillage as a function of soil organic matter content

... (P>50 mm) and small aggregates (P<5 mm) produced by ploughing as a function of the soil content of non-complexed material, NCM, as defined in ...

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Internal initiation of translation on the vesicular stomatitis virus phosphoprotein mRNA yields a second protein.

Internal initiation of translation on the vesicular stomatitis virus phosphoprotein mRNA yields a second protein.

... VSV mRNA was synthesized in vitro in a reaction mixture containing final concentration Tris hydrochloride pH 8.0 50 mM, NaCI 0.1 M, dithiothreitol 4 mM, MgCl2 5 mM, ATP, CTP, and GTP 1 m[r] ...

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Synthesis and in Vitro Antimalarial Activity of Alkyl Esters Gallate as a Growth Inhibitors of Plasmodium Falciparum

Synthesis and in Vitro Antimalarial Activity of Alkyl Esters Gallate as a Growth Inhibitors of Plasmodium Falciparum

... the five-carbon chain branched chain of isoamyl gallate with IC 50 value of 8.73 mM. Modification of the hydroxy group on the gallic acid with tmethoxy group will increase its antimalarial activity against ...

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Optimising nutrient extraction from chicken manure and compost

Optimising nutrient extraction from chicken manure and compost

... (a) 50 mm and (b) 100 mm columns, and mature compost in (c) 50 mm column and (d) 100 mm ...Figure 5-3 The effect of packing density on cumulative total dissolved nitrogen ...

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 THROMBOLYTIC AND MEMBRANE STABILIZING PROPERTIES OF METHANOLIC EXTRACTS OF AVICENNIA MARINA LEAVES & BARKS

 THROMBOLYTIC AND MEMBRANE STABILIZING PROPERTIES OF METHANOLIC EXTRACTS OF AVICENNIA MARINA LEAVES & BARKS

... with 5 ml hypotonic solution (50 mM NaCl) holding either the various concentration of crude methanolic extracts (2, 4, 6, 8 & 10 mg/ml respectively) or Acetyl Salicylic Acid ...

5

Corn yield response to partial rootzone drying and deficit irrigation strategies applied with drip system

Corn yield response to partial rootzone drying and deficit irrigation strategies applied with drip system

... (DI- 50) treatments received 100 and 50% of seven-day cumulative evaporation from Class A pan located at the experimental station, ...and 50% cumulative pan evaporation value, respectively, on one ...

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Wildland fire impacts on water yield across the Contiguous United States

Wildland fire impacts on water yield across the Contiguous United States

... flow relatively the most in the Lower Colorado, Pacific Northwest, and California regions. In the Lower Colorado and Pacific Northwest regions, flow increased despite post-fire drought conditions. In southern California, ...

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Antibacterial Potentials of Stem Bark Extracts of Leptadenia Lancifolia against some Pathogenic Bacteria

Antibacterial Potentials of Stem Bark Extracts of Leptadenia Lancifolia against some Pathogenic Bacteria

... Antibacterial activity the phytoconstituents of stem bark extracts of Leptadenia lancifolia extracted with various solvents (water, acetone and methanol) were investigated using the filter paper disc diffusion method. ...

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Integrin dependent homotypic adhesion of neutrophils  Arachidonic acid activates Raf 1/Mek/Erk via a 5 lipoxygenase  dependent pathway

Integrin dependent homotypic adhesion of neutrophils Arachidonic acid activates Raf 1/Mek/Erk via a 5 lipoxygenase dependent pathway

... (21, 22). Moreover, some effects of exogenous AA are inhibited by pertussis toxin (PTX) (21). Therefore, it is unclear whether AA acts directly via a G protein–linked cell surface receptor, or indirectly by serving as ...

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The influence of enhanced excavation base stiffness on prop loads and ground movements during basement construction

The influence of enhanced excavation base stiffness on prop loads and ground movements during basement construction

... The model wall had a flexural stiffness corresponding to a prototype concrete wall approximately 1.35m thick. A very stiff wall was used to minimise horizontal displacements caused by bending. The wall was manufactured ...

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Vaccinia virus gene A18R encodes an essential DNA helicase.

Vaccinia virus gene A18R encodes an essential DNA helicase.

... mM ATP–2 mM dithiothreitol–0.5 fmol of substrate–350 fmol of enzyme at 378C. The data in Fig. 2D show that under these conditions, the His-A18R helicase and ATPase activities re- mained linear for ...

9

European evidence based guidelines on pancreatic cystic neoplasms

European evidence based guidelines on pancreatic cystic neoplasms

... 40 mm, clinicians can incorporate other factors such as age, comorbid- ities, patient’s surgical risk, and patient ...<30 mm, it may be difficult to make a definitive diag- nosis of an MCN, and smaller ...

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Abortive and transforming infection of rat cell line 3Y1 by adenovirus type 12.

Abortive and transforming infection of rat cell line 3Y1 by adenovirus type 12.

... Number of transformed foci after infection of 3YI-B clone 1-6 cells with Adl2 as a function of the number of infected cells plated in a 50-mm petri disha No.. of cells seeded/50-mm petri[r] ...

5

Velocity distribution measurements in a fishway like open channel by Laser Doppler Anemometry (LDA)

Velocity distribution measurements in a fishway like open channel by Laser Doppler Anemometry (LDA)

... Figure 6a depicts that mean stream-wise velocity profiles for flow both installing a half cylinder (four regions; close to wall, 25, 50 and 100 mm ahead of the cylinder) and with without installing a half ...

5

The synergistic effects of high nitrate concentrations on sediment bioreduction

The synergistic effects of high nitrate concentrations on sediment bioreduction

... sediment with 0.3 mM initial nitrate T=50, B FeIII- reducing bicarbonate unamended... sediment with 10 mM initial nitrate T=50, C FeIII- reducing bicarbonate amended.[r] ...

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