A fumigatus
Characterization of a thermostable β-glucosidase from Aspergillus fumigatus Z5, and its functional expression in Pichia pastoris X33
... Nucleotide sequence analysis showed that the sequence of bgl3 contained an open reading frame (ORF) of 2622 bp. The deduced protein sequence of bgl3 contained an N-terminal signal sequence of 29 amino acids, as predicted ...
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Molecular Insights into Pathogenesis and Infection with Aspergillus Fumigatus
... A. fumigatus, proteomic analyses were carried out during a temperature shift from 30 ° C to 48 ° C, using a differential gel electrophoresis (DIGE) technique ...
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Microsatellite Markers for Typing Aspergillus fumigatus Isolates
... For the first time, the characterization of microsatellites of A. fumigatus is reported and the use of microsatellites as a typing system was investigated. These microsatellites are poly- morphic, and reproducible ...
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Phenotypic and genotypic analysis of variability in Aspergillus fumigatus
... Aspergillus genus. The repetitive DNA fragments observed in the gels were shown to be of mitochondrial origin by compar- ison with HaeIII-digested purified mtDNA patterns (51) and by hybridization with whole purified ...
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Global Population Genetic Analysis of Aspergillus fumigatus
... A. fumigatus, treatment failures are increasingly common and drug- resistant aspergillosis has become a significant global health issue (4, ...A. fumigatus strains from different geographic areas ...
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Aspergillus fumigatus: Principles of Pathogenesis and Host Defense
... Experimental studies performed in the early 1980s in immu- nocompromised mice indicate a critical role for macrophages in conidial defense and postulate that neutrophils act primarily against products of germination ...
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Impact of Aspergillus fumigatus in allergic airway diseases
... A. fumigatus antigens (discussed in more depth below), and increased peripheral blood eosinophilia ...A. fumigatus hypersensitivity and asthmatic ...
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A Nonredundant Phosphopantetheinyl Transferase, PptA, Is a Novel Antifungal Target That Directs Secondary Metabolite, Siderophore, and Lysine Biosynthesis in Aspergillus fumigatus and Is Critical for Pathogenicity
... pptA null mutant. Márquez-Fernández et al. in 2007 (31) defined a role for CfwA/NpgA in the production of NRPS- and PKS-derived secondary metabolites in A. nidulans. As secondary metabolites are thought to play a key role ...
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Factors affecting germination of ascospores in Aspergillus fumigatus
... studied thermotolerance of both conidia and ascospores of A. fischeri var. glaber. Their findings demonstrated an apparent contrast between ascospore germination of A. fumigatus and A. fischeri. Since dormant ...
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Identification and Characterization of an Aspergillus fumigatus “Supermater” Pair
... Recombinational analysis. To confirm whether the super- mater pair is also suitable for recombinational analysis, we created deletion mutant forms of the alb1 (pksP) gene, AFUA_2G17600, in MAT-2 strain AFIR928, and the ...
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A Novel Phosphoregulatory Switch Controls the Activity and Function of the Major Catalytic Subunit of Protein Kinase A in Aspergillus fumigatus
... A. fumigatus PkaC1 signifies key regulatory roles for phosphorylation in PkaC1 function and ...A. fumigatus PkaC1. As the S. cerevisiae and A. fumigatus proteins are highly conserved within the ...
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Detection of Airway Colonization by Aspergillus fumigatus by Use of Electronic Nose Technology in Patients with Cystic Fibrosis
... A. fumigatus, using the method of analysis from our previous study ...A. fumigatus) and calculating the chance that a random classification would have resulted in the same cross-validated accuracy or ...
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Isolation and Characterization of Clinical Triazole Resistance Aspergillus fumigatus in Iran
... All 172 isolates of A. fumigatus were subculture from the primary culture onto a four-well agar plate containing 4 mg/L of ITC, 1 mg/L of VRC and 0.5 mg/L of POS. The ability to grow was evaluated after 48 h. Any ...
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Chlorpyrifos Removal Improvement In Liquid Media By Aspergillus Fumigatus
... A. fumigatus is a fungus that confers an important role in the recycle of carbon and nitrogen in nature, due to its ability to utilize the several sources for supporting ...
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Pathway of Glycine Betaine Biosynthesis in Aspergillus fumigatus
... A. fumigatus seems to use GB as a sole source of carbon, nitrogen, and energy through suc- cessive demethylations to ...A. fumigatus during early conidial germination indeed revealed an upregulation of ...
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Multilocus Sequence Typing of the Pathogenic Fungus Aspergillus fumigatus
... A. fumigatus may be a separate, more recently evolved species ...A. fumigatus iso- lates were reclassified as ...A. fumigatus has low interstrain variation in its genome, suggesting recent evolution ...
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AtrR Is an Essential Determinant of Azole Resistance in Aspergillus fumigatus
... ChIP-seq. Chromatin immunoprecipitation (ChIP) was done as described in reference 13. Purified ChIP-ed DNA was then subjected to library preparation as outlined in reference 33, with the following modifications. The ...
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The genomics of Aspergillus fumigatus
... A. fumigatus also produces at least four phospholipases (Birch et al 1996) may have a role in invasive ...A. fumigatus, but it is not thought to be a major factor in virulence of the ...
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Biosynthesis of β-(1→5)-Galactofuranosyl Chains of Fungal-Type and O-Mannose-Type Galactomannans within the Invasive Pathogen Aspergillus fumigatus
... The phenotypic abnormalities in the growth of the ΔgfsA strain were less severe than those of the ΔgfsAC and ΔgfsABC strains (Fig. 6; see also Tables 3 and 4). Deletion of gfsB and gfsC did not result in any growth ...
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Molecular Studies Reveal Frequent Misidentification of Aspergillus fumigatus by Morphotyping
... A. fumigatus clinical culture collections in the United States using a two-step screening process that incorpo- rates restriction fragment length polymorphism (RFLP) anal- ysis followed by multilocus sequence ...
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