α galactosidase
Efficacy and tolerability of α-galactosidase in treating gas-related symptoms in children: a randomized, double-blind, placebo controlled trial
... enzyme, α-galactosidase, derived from Asper gillus niger mold, acts in the gastrointestinal tract by breaking down specific non-absorbable oligosaccharides before they are metabolized by colonic bacteria ...
7
Valuation of agro-industrial wastes as substrates for heterologous production of α-galactosidase
... influence, individual and combined, of the factors (inde- pendent variables) cheese whey and beet molasses con- centration, on the response (dependent variable) that is quantified as the extracellular ...
13
Role of glycosylation in secretion and stability of micromycetes α-galactosidase
... niger α-galactosidase, however reduced yield of Cladosporium cladosporioides and Penicillium canescens ...of α-galactosidases of all three producers ...niger α-galactosidase, 3.3 and ...
8
Characterization of a highly stable α-galactosidase from thermophilic Rasamsonia emersonii heterologously expressed in a modified Pichia pastoris expression system
... thermophilic α-galactosidase ...exhibited α-galactosidase activities as demonstrated by the remarkable hydrolytic activities toward pNPGal in the culture supernatant of the respective ...nant ...
13
Thermus thermophilus as source of thermozymes for biotechnological applications: homologous expression and biochemical characterization of an α-galactosidase
... Thermus thermophilus HB27 strain was purchased from DSMZ. A frozen (−80 °C) stock culture was streaked on a Thermus Medium (TM) solidified by the addition of 0.8% Gelrite ® (Sigma) and incubated at 70 °C overnight [58]. ...
10
Effect of Domestic Processing and Crude Extract of α-Galactosidase on Oligosaccharide Content of Red Gram (Cajanus cajan L.) Seeds
... The α -galactosidase enzyme hydrolyses the galactose from raffinose family oligosaccharides. Several studies reported the effect of domestic processing on oligosaccharide content of pulses and beans. ...
10
Influence of chemical reagents and UV irradiation on the activity of Penicillium canescens α-galactosidase
... canescens α-galactosidase is a homohexamer with the molecular mass 400 kDa ...canescens α-galactosidase cross-linked by glutar- aldehyde [19] are considerably inferior in activity to the ...
9
Optimization of Saccharomyces cerevisiae α-galactosidase production and application in the degradation of raffinose family oligosaccharides
... the α-factor was 45–50% with respect to the total activity at 120 h, secretion directed by the native signal was 30% over the same time interval ...the α-galactosidase activity associated with the ...
17
α-D-Gal-cyclophellitol cyclosulfamidate is a Michaelis complex analog that stabilizes therapeutic lysosomal α-galactosidase A in Fabry disease
... that α-gal-cyclosulfate 2 would covalently and irreversibly inhibit α-gal A with equal efficiency and selectivity following the same mode of action (Figure 1B and ...competitive α-gal A inhibitors ...
12
α-Galactosidase A-Tat Fusion Enhances Storage Reduction in Hearts and Kidneys of Fabry Mice
... of α -galactosidase A ( α -gal A)-Tat fusion protein for correction of Fabry disease, the second-most prevalent LSD with manifestations in the brain, kidney and ...similar α-gal A enzyme ...
6
Identification of an intestine-specific promoter and inducible expression of bacterial α-galactosidase in mammalian cells by a lac operon system
... bacterial α-galactosidase can also be successfully expressed in mammalian cells and that α-galactosidase activity could be measured at about 24 ...that α-galactosidase supple- ...
10
Stability of native and modified α-galactosidase of Cladosporium cladosporioides
... obtained α-galactosidase (ЕС ...cladosporioides α-galactosidase, arabinose and rhamnose have also been found, and their amounts ...porioides α-galactosidase permits estimating ...
8
Safety and pharmacodynamic effects of a pharmacological chaperone on α-galactosidase A activity and globotriaosylceramide clearance in Fabry disease: report from two phase 2 clinical studies
... GL-3 was measured in skin and kidney tissues with LC- MS/MS under non-GLP conditions on aqueous samples derived from tissue homogenates (MDS Pharma Ser- vices, Montreal, Canada). The homogenization protocol was the same ...
11
Fabry disease: Identification of 50 novel α-galactosidase A mutations causing the classic phenotype and three-dimensional structural analysis of 29 missense mutations
... Fabry disease, an X-linked recessive inborn error of glycosphingolipid catabolism, results from the deficient activity of the lysosomal exoglycohydrolase, a-galactosidase A (EC 3.2.1.22; a-Gal A). The molecular ...
13
Fabry Disease: Twenty Novel α-Galactosidase A Mutations and Genotype-Phenotype Correlations in Classical and Variant Phenotypes
... Six novel small gene rearrangements, including two single nucleotide insertions and three dele- tions were identified in six unrelated classically affected probands (Table 2).. The three[r] ...
7
Fabry Disease: Thirty-Five Mutations in the α-Galactosidase A Gene in Patients with Classic and Variant Phenotypes
... The dinucleotide deletions included the 1057de12 mutation, which caused a frameshift in codon 353 and termination of the polypeptide at residue 373 of exon 7, and the 1074del2 muta- tion[r] ...
9
VASCULAR DYSFUNCTION IN THE Α GALACTOSIDASE A KNOCKOUT MOUSE IS AN ENDOTHELIAL CELL , PLASMA MEMBRANE BASED DEFECT
... 1. Fabry disease results from an X-linked mutation in the lysosomal -galactosidase A (Gla) gene. Defective Gla results in multi-organ accumulation of neutral glycosphingolipids (GSLs), especially in the vascular ...
8
Twenty Novel Mutations in the α-Galactosidase A Gene Causing Fabry Disease
... Analysis of the a-Gal A gene in 30 unrelated probands with Fabry disease identified 20 new mutations and 9 previously reported lesions.. In addition, all 28 mutations were confirmed in g[r] ...
6
Non-contiguous finished genome sequence and description of Kallipyga massiliensis gen. nov., sp. nov., a new member of the family Clostridiales Incertae Sedis XI
... Kallipyga (cal.li.pi’ga N.L. fem. N. Kallipyga of the Greek epithet kallipygos , said of a statue of Aphro- dite having beautifully proportioned buttocks). Gram-positive cocci. Strictly anaerobic. Mesophilic. Non-Motile. ...
16
Soil hydrolase activities and kinetic properties as affected by wheat cropping systems of Northeastern China
... (β-galactosidase, α-galactosidase, β-glucosidase, α-glucosidase, urease, protease, phosphomonoesterase, phosphodiesterase, aryl- sulphatase) to different wheat cropping system; (2) soil enzyme ...
7