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Follicle-stimulating hormone responsiveness in antral follicles from aryl hydrocarbon receptor knockout mice

... Follicle growth was evaluated in cultured antral follicles as previously described [6,20]. Briefly, early antral follicles from WT and AHRKO mice on PDs 30–32 (3–6 mice per genotype per experiment) were placed ...

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Original Article Influence of different culture media on isolation of murine BMSCs

... tured in three different media: α-MEM, DMEM and DMEM/F12, each was supplemented with 10% FCS. The vessels were placed at 37°C in a humidified atmosphere containing 5% CO 2 . The first medium change was ...

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The influence of cerebrospinal fluid on epidermal neural crest stem cells may pave the path for cell-based therapy

... The influence of CSF on characteristics of isolated EPI-NCSCs was investigated with immunocytochemistry and real-time PCR. Immunostaining of expanded EPI- NCSCs after 72 hours of cultivation in CSF indicated that these ...

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Native extracellular matrix preserves mesenchymal stem cell “stemness” and differentiation potential under serum-free culture conditions

... Three-dimensional; α -MEM: Alpha-minimum essential medium; ALP: Alkaline phosphatase; ANOVA: Analysis of variance; BM: Bone marrow; BM-ECM: Bone marrow-derived extracellular matrix; BM-MSC: Bone marrow- ...

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Conditioned Mediums and Human Oocytes in Vitro Maturation

... [28].The α- MEM media was used as the basic control ...in α-mem medium (Gibco) supplemented with 10% FBS (Gibco), 100 mg/mL streptomycin (Gibco) and 100 U/mL penicillin (Gibco), in the ...

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Different media and supplements modulate the clonogenic and expansion properties of rabbit bone marrow mesenchymal stem cells

... either in α-MEM, or DMEM, or Medium199 (M199), or Mesenchymal Stem Cells Basal Medium (MSCBM). FCS 20%, Penicillin 100 U/mL, Streptomycin 0.1 mg/mL and L-Glutamine 2 mM were added to α-MEM, ...

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Effect of coculturing canine notochordal, nucleus pulposus and mesenchymal stromal cells for intervertebral disc regeneration

... fibrosus; α -MEM: Minimum essential medium Eagle, alpha modification; CD: Chondrodystrophic; COL1A1: Collagen, type I, α 1; COL2A1: Collagen, type II, α 1; DMEM: Dulbecco ’ s modified Eagle ’ ...

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Transcriptional profiling reveals intrinsic mRNA alterations in multipotent mesenchymal stromal cells isolated from bone marrow of newly-diagnosed type 1 diabetes patients

... α -MEM, minimum essential medium alpha; BRITE, binary relationships of biological entities; C-MSC, bone marrow-derived MSC isolated from healthy donors (controls); CKLF, chemokine-like factor; COX2, ...

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Ameliorating Effect of β-Carotene on Gene Expression Alteration in Spermatozoon Cultured With Titanium Oxide

... in α-MEM, containing ...in α-MEM medium and removing most interstitial cells, spermatozoa, and some spermatid cells, the second digestion step (45 minutes incubation at 32°C) was performed in ...

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Neurogenic potential of dental pulp stem cells isolated from murine incisors

... α -MEM: alpha-modified Eagle ’ s medium; ANOVA: analysis of variance; ChAT: choline acetyltransferase; CsGlutamine: caesium glutamine; Cx43: connexin 43; DAPI: 4 ’ ,6-diamidino-2-phenylindole; DPSC: dental ...

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Impaired osteogenesis in Menkes disease-derived induced pluripotent stem cells

... after α-MEM induction in the WT- iPSC group, whereas mesenchymal morphology was ob- served at 2 weeks in the MD1- and MD2-iPSC groups ...of α-MEM induction, but no difference was detected in ...

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Human ethmoid sinus mucosa: a promising novel tissue source of mesenchymal progenitor cells

... Chondrogenic differentiation was induced using the micromass culture technique. Briefly, 10 μl of a concen- trated MSC suspension (3 × 10 5 cells/ml) were plated into the center of each well and allowed to attach at 37°C ...

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Human endothelial and foetal femur-derived stem cell co-cultures modulate osteogenesis and angiogenesis

... phosphatase; α -MEM: minimal essential medium, α -modification; ANOVA: analysis of variance; BMP: bone morphogenic protein; BSA: bovine serum albumin; BS/BV: bone surface/volume ratio; CD31: cluster ...

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Synergistic effects on mesenchymal stem cell-based cartilage regeneration by chondrogenic preconditioning and mechanical stimulation

... α -MEM: α -Minimal essential medium; CIM: Chondrogenic induction medium; Col II: Type 2 collagen; DL: Dynamic compressive loading; FL: Free loading; GAG: Glycosaminoglycan; GFP: Green fl[r] ...

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Enhanced osteogenic potential of mesenchymal stem cells from cortical bone: a comparative analysis

... 3D: Three-dimensional; α -MEM: Alpha-modified Eagle ’ s medium; AD- MSC: Adipose tissue mesenchymal stem cell; ALP: Alkaline phosphatase; Bglap: Osteocalcin; BM-MSC: Bone marrow mesenchy[r] ...

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Prophylactic therapy with human amniotic fluid stem cells improved survival in a rat model of lipopolysaccharide-induced neonatal sepsis through immunomodulation via aggregates with peritoneal macrophages

... The study was approved by the Institutional Review Board of Keio University School of Medicine (no. 20140285), and informed consent was obtained from all the volunteer donors. Five-milliliter amniotic fluid sam- ples ...

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CCAAT/enhancer binding protein β promotes receptor activator of nuclear factor kappa B ligand (RANKL) expression and osteoclast formation in the synovium in rheumatoid arthritis

... -MEM: α -minimum essential medium; AP-1: activator protein-1; ATF-4: activation transcription factor 4; bp: base pairs; C/EBP: CCAAT/enhancer binding protein; ChIP: chromatin immunoprecipitation; DMEM: ...

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