α-Tubulin
The dual functions of α-tubulin acetylation in cellular apoptosis and autophage induced by tanespimycin in lung cancer cells
... of α-tubulin and HDAC6 inhi- bition enhance autophagy in non-small cell lung cancer ...acetylated α-tubulin and ...acetylated α-tubulin level, from which leads to cell death and ...
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Posttranslational modifications of α tubulin in alzheimer disease
... of tubulin-positive axons in neu- rons with or without NFTs in AD and control brain re- vealed that NFT-free neurons demonstrated similar length between AD and control, suggesting there is no specific effects of ...
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Role of α Tubulin Acetylation and Protein Kinase D2 Ser/Tyr Phosphorylation in Modulation by Ghrelin of Porphyromonas gingivalis Induced Enhancement in Matrix Metalloproteinase 9 (MMP 9) Secretion by Salivary Gland Cells
... its tubulin subunit acetylation on Lys 40 [11] [15], we have monitored the impact of ...acetylated α -tubulin in the acinar ...acetylated α -tubulin level was ob- served following the ...
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Neural development in the tardigrade Hypsibius dujardini based on anti-acetylated α-tubulin immunolabeling
... absolute methanol at 4°C for 20 min, then run through a methanol series (5 min each in 90%, 70%, 50% metha- nol). Fixation was done using 1 ml of 4% paraformalde- hyde in PBT for 10 min at room temperature followed by 15 ...
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α-Tubulin Mutations Alter Oryzalin Affinity and Microtubule Assembly Properties To Confer Dinitroaniline Resistance
... dinitroaniline-sensitive tubulin, and we investi- gated it as a system for expressing functional mutant tubulins for biochemical ...1- tubulin that confer dinitroaniline resistance in ...with ...
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Immunoglobulin E Binding Reactivity of a Recombinant Allergen Homologous to α-Tubulin from Tyrophagus putrescentiae
... the Uni-CAP system. The nonatopic sera from 20 subjects who were not sensitized to the 50 allergens tested were used as negative controls. Informed consent was obtained from the subjects before the experiments with the ...
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Trypanosoma cruzi Bromodomain Factor 3 Binds Acetylated α-Tubulin and Concentrates in the Flagellum during Metacyclogenesis
... The results presented here show that TcBDB3 binds to acety- lated ␣ -tubulin both ex vivo and in vivo. This interaction seems to be associated with changes in the amount of TcBDF3 in the flagel- lum during ...
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An α-Tubulin Mutant Demonstrates Distinguishable Functions Among the Spindle Assembly Checkpoint Genes in Saccharomyces cerevisiae
... meiosis causes aneuploidy, which can lead to deleterious When shifted to the nonpermissive temperature (15⬚), consequences such as cancer and birth defects. Microtu- cells expressing tub1-729, a class 1 allele, as its ...
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Inclusion Body Fusion of Human Parainfluenza Virus Type 3 Regulated by Acetylated α-Tubulin Enhances Viral Replication
... Viral inclusion bodies (IBs), or viral replication factories, which are accumulated aggregates of viral proteins, are commonly generated in a variety of animal viruses, such as DNA viruses (e.g., herpesviruses and ...
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Glutamylation on α-Tubulin Is Not Essential but Affects the Assembly and Functions of a Subset of Microtubules in Tetrahymena thermophila
... tal tubulin CTT peptide pools to characterize glutamylated forms of ciliary ␣ ...axonemal tubulin showed that glutamylation occurs predomi- nantly in ␣ -tubulin and that it is a major modification, ...
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Pironetin reacts covalently with cysteine 316 of α tubulin to destabilize microtubule
... of tubulin: vinblastine, colchicine and maytansine inhibit polymerization of the tubulin heterodimer into microtubules 2,3 while taxol and laulimalide promote polymerization and stabilize microtubules 2,4 ...
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TgATAT-Mediated α-Tubulin Acetylation Is Required for Division of the Protozoan Parasite Toxoplasma gondii
... Interestingly, many of the phenotypes we observed upon the disruption of K40 ␣ -tubulin acetylation parallel the effects observed when microtubules are destabilized with oryzalin (13, 14, 39, 56, 57). Treatment of ...
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α-Tubulin Minichromosome Promoters in the Stichotrichous Ciliate Stylonychia lemnae
... 1 tubulin minichro- mosome, mutation of the sequence from position ⫺ 4 to posi- tion ⫺ 11 did not affect the accuracy or efficiency of transcrip- tion ...1 tubulin minichro- mosome did not abolish ...
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Evaluation of α tubulin, detyrosinated α tubulin, and vimentin in CTCs: identification of the interaction between CTCs and blood cells through cytoskeletal elements
... Triple-staining experiments revealed that the ratios CK/TUB, CK/GLU, and CK/VIM were significantly increased in the well-differentiated HR+ MCF7 cells compared with the more aggressive c[r] ...
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Screen based identification and validation of four new ion channels as regulators of renal ciliogenesis
... (acetylated α -tubulin, red), tight junctions (ZO-1, white), and adherens junctions ( β -catenin, green) with DAPI counterstaining (blue) showing loss of cilia after Kcnq1 siRNA transfection (20 nM), and ...
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Neuromuscular study of early branching Diuronotus aspetos (Paucitubulatina) yields insights into the evolution of organs systems in Gastrotricha
... Longitudinal nerves Anterior to the brain neuropil of Diuronotus aspetos, four pairs of dorsal nerve projec- tions are found (acetylated α-tubulin-LI-reactive, in addition to several minor neurites left ...
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Cell-cycle-specific Cellular Responses to Sonoporation
... whose α-tubulin cytoskeleton was labeled by incorporation of a green fluorescence protein (GFP)-α-tubulin fusion protein (referred as GFP-α-tubulin HeLa cells), were synchronized ...
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PIWIL1 destabilizes microtubule by suppressing phosphorylation at Ser16 and RLIM-mediated degradation of stathmin1
... PIWIL1 shRNA: 5’-GCCGTTCATACAAGACTA ATT-3’ STMN1 shRNA: 5’-AGGCAATAGAAGAGAAC AA-3’ CaMKII shRNA: 5’-CCACTACCTTATCTTCGAT-3’ CaMKIV shRNA: 5’-GATGGCAACGAGGACAT GA-3’ RLIM shRNA: 5’-GGTCTCAGACACCAAACAA CA-3’ The antibodies ...
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The synergic effect of vincristine and vorinostat in leukemia in vitro and in vivo
... for tubulin deacetylation, which plays a key regulatory role in the stability of the dynamic microtubules ...factor-κβ, α-tubulin, heat shock protein 90, and Ku 70 ...
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Screen-based identification and validation of four new ion channels as regulators of renal ciliogenesis
... (acetylated α -tubulin, red), tight junctions (ZO-1, white), and adherens junctions ( β -catenin, green) with DAPI counterstaining (blue) showing loss of cilia after Kcnq1 siRNA transfection (20 nM), and ...
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