Actin-binding domain (ABD)
Functional correction of dystrophin actin binding domain mutations by genome editing
... cardiomyopathy, and a shortened lifespan. Mutations of dystrophin that disrupt the amino- terminal actin-binding domain 1 (ABD-1), encoded by exons 2–8, represent the second- most common cause of ...
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Development and Synthesis of Utrophin Actin Binding Domain 1 (ABD1)
... purification of this protein will be critical for thermodynamic analysis of the actin binding domain of Utrophin so it can be compared with Dystrophin. It is hypothesized that Utrophin will display ...
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The C Terminus of Rotavirus VP4 Protein Contains an Actin Binding Domain Which Requires Cooperation with the Coiled-Coil Domain for Actin Remodeling
... and actin cytoskeleton have been widely ...apical domain of infected polarized enterocytes and favor the polarized apical exit of viral ...of actin-VP4 ...with actin microfilaments. The VP4 ...
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Molecular analysis of insertion/deletion mutations in protein 4 1 in elliptocytosis I Biochemical identification of rearrangements in the spectrin/actin binding domain and functional characterizations
... normal 4.1. These studies have thus enabled us to establish the importance of the spectrin-actin binding domain in regulating the mechanical stability of the erythrocyte membrane. 86:516[r] ...
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Actin Binding Domains Direct Actin-Binding Proteins to Different Cytoskeletal Locations
... its actin-binding domain (ABD) and examined the localization of these fluorescent proteins in living cells under a variety of ...GFP-actin-binding domain fusion proteins ...
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Imaging of Dynamic Changes of the Actin Cytoskeleton in Microextensions of Live NIH3T3 Cells with a GFP Fusion of the F-Actin Binding Domain of Moesin
... C-moesin-GFP as a Probe to Study Filopodial Microfila- ments By viewing cells for periods of up to several hours, and by comparing time-lapse video images obtained by DIC with fluorescence images we concluded that the ...
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Hypertrophic cardiomyopathy mutations in the calponin-homology domain of ACTN2 affect actin binding and cardiomyocyte Z-disc incorporation
... purified actin binding domain of ACTN2 by circular dichroism and X-ray crystallography, and show small but distinct changes for both ...F- actin binding affinity, although the ...
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Regulation of myotube formation by the actin-binding factor drebrin
... an actin-depolymerizing factor II/cofi- lin-like domain, an actin-binding domain and two Homer-binding domains [27], and it remodels actin to facilitate the maturation of ...
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Emerging roles of TRIO and F-actin-binding protein in human diseases
... major actin-binding domain of TRIOBP-4/5 [2, 10]. TRIOBP-4/5 is the protein that plays a pivotal role in the formation of rootlets, because Triobp-4 knockout mice cannot form rootlets. In addition, ...
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The Nucleocapsid Domain of Gag Is Dispensable for Actin Incorporation into HIV-1 and for Association of Viral Budding Sites with Cortical F-Actin
... of actin filaments. Actin was found to interact with the nucleocapsid domain of the viral structural protein Gag and is incorporated in significant amounts into HIV-1 particles, suggesting that it ...
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AFM Investigation of the Organization of Actin Bundles Formed by Actin Binding Proteins
... G- actin monomers) and ...the actin bundle was diluted in 200 µL of polymerization buffer and deposited in the tube on top of the freshly cleaved ...
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Interactions of actin, myosin, and an actin binding protein of chronic myelogenous leukemia leukocytes
... https://doi.org/10.1172/JCI108373. Actin, myosin, and a high molecular weight actin-binding protein were purified from chronic myelogenous leukemia (CML) ...leukocyte actin resembled skeletal ...
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The Biologically Relevant Targets and Binding Affinity Requirements for the Function of the Yeast Actin-Binding Protein 1 Src-Homology 3 Domain Vary With Genetic Context
... single domain with many proteins are controlled and modulated under varying cellular ...SH3 domain mutants that incrementally reduce target-binding affinity in four different yeast mutant backgrounds ...
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Integration of the Rac1- and actin-binding properties of Coronin-1C
... the actin-binding protein, coronin- 1C, as a key regulator of Rac1 trafficking that was necessary for Rac1 activation at the leading ...an actin binding site, and is required for membrane ...
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Thymosin β as an Actin-binding Protein with a Variety of Functions
... muscle actin) and endothelial specific receptor Tie2 have been identified on the surface of this organ, thus it can be concluded that the purpose of these cells was to transform into endothelial cells ...
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EPIgeneousTM Binding Domain Kit A
... GST-binding domain of interest has already been validated, inhibitor titration experiments can be performed directly using the recommended conditions (kit and peptide-biotin concentration) described in the ...
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Involvement of GTP-binding proteins in actin polymerization in human neutrophils
... Cells preincubated with pertussis toxin (400 ng/ml), which totally inhibits chemotatic peptide- induced actin polymerization (12, 13), exhibited an almost unaffected increase in F-actin [r] ...
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Ca'-dependent Binding of Severin to Actin : A One-to-One Complex Is
... Effect of Severin on the Reannealing of F-Actin: The viscosity ofeither actin alone, or actin with severin present at a 7:1 ratio was measured in F-buffer containing either added 0.5 mM [r] ...
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Genetic analysis of the fimbrin-actin binding interaction in Saccharomyces cerevisiae.
... Although we favor the notion that the two actin-bind- ing domains of Sac6p do bind to the same site on actin, we suggest there are differences between the two do- mains t[r] ...
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Are the Conserved Sequences in Segment 1 of Gelsolin Important for Binding Actin?
... This may explain our in- ability to demonstrate similar effects with COOH-terminally deleted mutants of gelsolin : there was no detectable actin binding when Sl had been truncated to Glu[r] ...
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